Anti-inflammatory Effect of an Ethyl Acetate Fraction from Myagropsis yendoi on Lipopolysaccharides-stimulated RAW 264.7 Cells

Abstract

An ethanolic extract from Myagropsis yendoi was fractionated using several solvents. Among these, an ethyl acetate fraction (Myagropsis yendoi ethyl acetate fraction: MYE) showed the highest anti-inflammatory activity based on inhibition of lipopolysaccharides (LPS)-induced nitric oxide (NO) production in RAW 264.7 cells. We thus investigated the molecular mechanisms underlying MYE's inhibitory effects. Pretreatment of cells with up to 30 µg/mL of MYE significantly inhibited NO production and inducible nitric oxide synthase expression in a dose-dependent manner (P<0.05). Similarly, MYE markedly reduced the production of pro-inflammatory cytokines, such as interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α, as well as their mRNA levels. While the nuclear translocation of nuclear factor-kappa B (NF-κB) was strongly suppressed by MYE, the activation of a nuclear factor erythroid 2-related factor (Nrf2) was increased. Moreover, MYE significantly reduced the phosphorylation of JNK, p38 MAPK, and phosphatidylinositol 3-kinase/Akt in LPS-stimulated cells. These results indicate that MYE contains anti-inflammatory compounds, and that it might be used as a dietary supplement for the prevention of inflammatory diseases.

Phlorotannins Isolated from Eisenia bicyclis and Lactobacillus casei Ameliorate Dextran Sulfate Sodium-Induced Colitis in Mice through the AhR Pathway

“…However, mice treated with the combination of TA and LC showed significant alleviation of the disease and improved treatment effects. Phlorotannins isolated from E. Stolonifera have previously demonstrated anti-inflammatory effects by inhibiting IL-1β, IL-6, and TNF-α, along with a reduction in NF-κB expression [45,46]. In the current study, the synergistic combination of TA and LC reduced inflammation, particularly in parameters such as spleen enlargement and lipocalin 2, Cox2, Occludin, and STAT3 mRNA expression, which are involved in inflammatory modulation and tight junction regulation.…”

Anti-Inflammatory Effects of Grasshopper Ketone from Sargassum fulvellum Ethanol Extract on Lipopolysaccharide-Induced Inflammatory Responses in RAW 264.7 Cells

“…Inflammation is a complex biological response of the body for protection of damaged tissue against bacterial infection or pathogens [1]. In a general inflammatory response, pro-inflammatory mediators are decreased after antigens are removed, while anti-inflammatory mediators are increased.…”

“…The activated macrophages release excessive amounts of NO and prostaglandins (PGs) which are generated by iNOS (inducible nitric oxide synthase) and COX (cyclooxygenase)-2, respectively, as well as pro-inflammatory cytokines like interleukin (IL)-6, IL-1β, and tumor necrosis factor-α (TNF-α). These signaling pathways are able to induce inflammatory reactions, resulting in the development of various inflammationrelated diseases [1].…”

Effects ofHouttuynia cordataExtracts of Different Aerial Parts on Antioxidants and Anti-inflammatory

“…ROS는 세포 내 DNA, RNA, 단백질 등과 반 응하여 세포 손상 및 파괴 등을 유발하여 (Aischer and Hess, 1993;Chen et al, 2012 (Decker et al, 1992 (Aischer and Hess, 1993;Wiseman, 1996). (Kim et al, 2014;Seo et al, 2015). 어성초의 추출물은 사람의 B 림프구 및 T 림프구의 활성을 증가시키며 (Chun, 1997), insulin-like growth factor (IGF)-γ와 lipopolysaccharide (LPS)와 같은 면역조절제에 의해 대식세포가 자극이 되었 을 경우 시토카인의 분비량을 증가시킴으로서 면역반응을 향상시킨다는 보고가 있다 (Kim et al, 2005 -91 -al., 1975;Gerriten, 1995…”