Anti-Inflammatory Effect of Flavonoids from Brugmansia arborea L. Flowers

Abstract

Brugmansia arborea L. (Solanaceae), commonly known as "angel's trumpet," is widely grown in North America, Africa, Australia, and Asia. It has been mainly used for ornamental purposes as well as analgesic, anti-rheumatic, vulnerary, decongestant, and anti-spasmodic materials. B. arborea is also reported to show anti-cholinergic activity, for which many alkaloids were reported to be principally responsible. However, to the best of our knowledge, a phytochemical study of B. arborea flowers has not yet been performed. Four flavonol glycosides (1-4) and one dihydroflavanol (5) were for the first time isolated from B. arborea flowers in this study. The flavonoids showed significant antioxidant capacities, suppressed nitric oxide production in lipopolysaccharide (LPS)-treated RAW 264.7 cells, and reduced inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX-2) protein production increased by LPS treatment. The contents of compounds 1-4 in n-BuOH fraction were determined to be 3.8 ± 0.9%, 2.2 ± 0.5%, 20.3 ± 1.1%, and 2.3 ± 0.4%, respectively, and that of compound 5 in EtOAc fraction was determined to be 12.7 ± 0.7%, by HPLC experiment. These results suggest that flavonol glycosides (1-4) and dihydroflavanol (5) can serve as index components of B. arborea flowers in standardizing anti-inflammatory materials.

Antidiabetic effects of Brugmansia aurea leaf extract by modulating the glucose levels, insulin resistance, and oxidative stress mechanism

“…The research presented in this article aimed to identify and report the potential antidiabetic effects of specie B. aurea by reducing insulin resistance in animals with the disease. Previous studies reported the anti-inflammatory ( 14 , 17 ) and anticholinergic activities ( 18 ) of this plant. However, no studies are available on its antidiabetic activity.…”

“…( 12 , 13 ). Flavonoids from B. aurea have previously been reported to possess antioxidant activity ( 14 ).…”

Coreolanceolins A–E, New Flavanones from the Flowers of Coreopsis lanceolate, and Their Antioxidant and Anti-Inflammatory Effects

“…When inflammation occurs in response to activating signals such as LPS and/or cytokines, iNOS produces NO in macrophages [13,33,36]. In addition, the level of COX-2, a pro-inflammatory enzyme, can also indicate the extent of inflammation [13,36,37]. The effect of the flavanones on iNOS and COX-2 expression was evaluated using western blot experiments.…”

Evaluación preliminar in vivo de la actividad analgésica de las flores de Brugmansia arborea “floripondio”

“…Las plantas pertenecientes a la familia Solanaceae han sido ampliamente estudiadas desde el punto de vista farmacológico y toxicológico (Torres-Nagera et al, 2013). Algunas especies por décadas se han utilizado en la medicina tradicional basándose en el conocimiento empírico como es el caso de Brugmansia arborea "floripondio" que se cree posee propiedades analgésicas (Kim et al, 2020) en esta región la población la utiliza para tratar desde pequeños golpes hasta dolores musculares (Arteaga de García et al, 1993).…”

“…Según Caballero-Serrano et al (2019), B. arborea es ampliamente conocida en la medicina tradicional como una planta mágica, sin embargo agregan usos para tratar distintos dolores. Con base en estudios fitoquímicos (Mattioli et al, 2012;Kim et al, 2020;Algradi et al, 2021) realizados a la especie (Brugmansia arborea) se han encontrado alcaloides tales como: la atropina, hioscina, noratropina (Arteaga de García et al, 1993), hiosciamina y escopolamina (Mattioli et al, 2012), de los que en algunos casos ya se les atribuye una actividad analgésica y antiinflamatoria (Addams & Wang, 2015). Del mismo modo, Kim et al, (2020) determinaron la presencia de flavonoides como kaempferin, kaempferitrin, kaempferol, quercetin, y (+)-aromadendrin; varios de los cuales ya fueron reportados por su efecto antinociceptivo (De Melo et al, 2009, Toker et al, 2004, Almeida-Junior et al, 2020.…”