Anti-inflammatory effect of gangliosides in the rat hindpaw edema test

Correa, Silvia G.; Bianco, Ismael D.; Riera, Clelia M.; Fidelio, Gerardo D.

doi:10.1016/0014-2999(91)90640-c

Cited by 19 publications

(5 citation statements)

References 24 publications

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“…Changes in thickness were measured at 30 min. Edema caused by PLA 2 peaked at 10 min and remained elevated up to 60 min [29]. Administration of Gal-1 prior to the injection of PLA 2 significantly suppressed the development of swelling (Fig.…”

Section: Gal-1 Specifically Inhibits Pla 2 -But Not Histamine-inducedmentioning

confidence: 89%

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Evidence of a role for galectin-1 in acute inflammation

et al. 2000

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Section: Gal-1 Specifically Inhibits Pla 2 -But Not Histamine-inducedmentioning

confidence: 89%

“…Swelling, evaluated as changes in the thickness of the injected footpad, was measured using calipers at the indicated times. Data were expressed as the difference between the diameters at time zero and readings taken after the injection [29,48].…”

Section: Hind Paw Edemamentioning

confidence: 99%

Evidence of a role for galectin-1 in acute inflammation

et al. 2000

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“…Recent investigations of Bianco et al (1990Bianco et al ( , 1991 on artificial membranes demonstrated that gangliosides obtained from bovine brain have inhibitory effects on PLA 2 activity and Correa et al (1991) reported that the anti-inflammatory effect observed with gangliosides in reactions produced with different antigens is mediated by inhibition of PLA 2 . In this way, the inhibitory effect of total brain gangliosides and quinacrine, a well-known PLA 2 inhibitor (Connelly & Kierszenbaum, 1984;Hazlett et al 1989), on the destabilizing activity of T. cruzi, in addition to the lipid alterations on the erythrocyte membrane during the interaction with the parasite, supports the hypothesis that PLA 2 is implicated in this process.…”

Section: Discussionmentioning

confidence: 99%

“…In other cases, both cells were pre-treated with PLA 2 activity inhibitors: quinacrine 0-02 mM (Connelly & Kierszenbaum, 1984;Hazlett, Deems & Dennis, 1989) or total brain gangliosides 0'5 mM (Bianco, Fidelio & Maggio, 1989, 1990Correa et al 1991). In other experiments, phospholipase A 2 inhibitors were added to the incubation medium during parasite-erythrocyte interaction at the same concentration utilized in the pre-treatments.…”

Section: Effect Of Pla 2 Bsa and Pla 2 Inhibitorsmentioning

confidence: 99%

Intermembrane lipid transfer duringTrypanosoma cruzi-induced erythrocyte membrane destabilization

Luján

Bronia²

1994

Parasitology

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The ability of Trypanosoma cruzi to induce erythrocyte membrane destabilization in vitro was studied. Epimastigote forms adhered to human erythrocytes and caused fusion or lysis of the red cells, depending on the conditions of the interaction. Red cells were fused in the presence of calcium, while haemolysis was induced in the absence of the cation. Dextran 60 C facilitated fusion but delayed lysis. Optimum pH and temperature for fusion were 7.4 and 37 degrees C, respectively. Lipid alterations were produced in the plasma membrane of the red cell during the interaction with the parasite. A Ca(2+)-independent increase of lysophospholipids and free fatty acids was common to both the lysis and fusion processes. A relative increase of 1,2-diacylglycerides was unique to the fusion process and these changes were dependent on Ca2+. The transfer of free fatty acids and lysophospholipids from T. cruzi to erythrocyte membranes was demonstrated using parasites pre-labelled with radioactive phospholipids. Pre-treatment of parasites with exogenous phospholipase A2 abolished the fusogenicity, while lysis was increased. Neither fusion nor haemolysis occurred when the parasites were pre-treated with fatty acid free albumin, phospholipase A2 inhibitors or when these compounds were present in the medium during the parasite-erythrocyte interaction. Our results suggest that T. cruzi induces erythrocyte membrane destabilization in vitro by transfer of lipid material in a calcium independent manner and that this ion is necessary for other membrane alterations that lead to erythrocyte fusion.

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“…Ganglioside GD3 stimulates T cell activation in human peripheral blood lymphocytes (15,18). The results from several in vitro and in vivo studies suggest that GGs may have anti-inflammatory properties (19)(20)(21). When human epithelial cells are incubated with buffalo milk GGs, prostaglandin E2 (PGE2) production is inhibited by 75% in response to TNF-a (22).…”

mentioning

confidence: 99%