Anti-Inflammatory Effect of Immature Sword Bean Pod (<i>Canavalia gladiata</i>) in Lipopolysaccharide-Induced RAW264.7 Cells

Hwang, Kyung‐A; Heo, Wan; Hwang, Hye-Jeong; Han, Bok Kyung; Song, Myoung‐Chong; Kim, Young Jun

doi:10.1089/jmf.2020.4733

Cited by 11 publications

(17 citation statements)

References 40 publications

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“…Gallic acid is known as a phenolic acid component that has a high anti-obesity effect, and it is speculated that this is why the SBP extract has a higher anti-obesity effect than the SB extract. [31][32][33][34] Our results demonstrate that SB and SBP extracts inhibit fat production and increase the expression of thermogenic factors to prevent obesity via fat catabolism. In particular, the SBP extract showed a greater protective efficacy against obesity than that of SB and is expected to be a valuable raw material for product development aimed at controlling obesity.…”

Section: Discussionmentioning

confidence: 62%

Immature sword bean pods (Canavalia gladiata) inhibit adipogenesis in C3H10T1/2 cells and mice with high-fat diet–induced obesity

Hwang

Kim

et al. 2022

Journal of the Chinese Medical Association

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Background: Sword bean (SB; Canavalia gladiata) is a perennial vine used as a food and medicinal plant in Asia. SB is rich in nutrients, such as flavonoids and urease, and has various functions, including beneficial effects on dysentery, nausea, and hemorrhoids, as well as anti-inflammatory and antioxidant activity. Various plant parts are used; however, little is known about the physiological effects of SB pods (SBP). In this study, the anti-obesity effects of SBP extract were evaluated. Methods: To investigate the anti-obesity effects of SBP extract, we confirmed the SBP extract downregulated lipogenesis-related genes and upregulated genes involved in lipolysis and brown adipocyte markers in differentiated C3H10T1/2 adipocytes in vitro. Next, we use a high-fat diet (HFD)-induced obesity mouse model to determine the anti-obesity effects of SBP extract. Results: Treatment with SBP extract significantly reduced adipocytes. The extract decreased the HFD-induced increases in body weight and plasma triglyceride levels in mice after 8 weeks. mRNA and protein levels of the adipogenesis and lipogenesis-related factors CCAAT/enhancer binding protein-β, CCAAT/enhancer binding protein-α, peroxisome proliferator-activated receptor-γ (PPARγ), and their target genes Ap2, SREBP-1c, FAS, and SCD-1 were reduced by SBP extract. In contrast, AMP-activated protein kinase and sirtuin1, involved in the thermogenic catabolism of fat, were activated by SBP extract in adipocytes and white adipose tissue, increasing the expression of peroxisome proliferator-activated receptor gamma coactivator-1α, peroxisome proliferator-activated receptor-α (PPARα), and uncoupling protein 1 and activating thermogenic activity. Conclusion: SBP extract exerts an anti-obesity effect by inhibiting lipogenesis-related factors and activating fat-catabolizing factors; it is, therefore, a promising functional food and natural anti-obesity agent.

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Section: Discussionmentioning

confidence: 62%

Immature sword bean pods (Canavalia gladiata) inhibit adipogenesis in C3H10T1/2 cells and mice with high-fat diet–induced obesity

Hwang

Kim

et al. 2022

Journal of the Chinese Medical Association

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“…According to previous studies, DDP‐induced nephrotoxicity could increase the expression levels of pro‐inflammatory factors (iNOS, COX‐2, TNF‐α, and IL‐1β) and decrease the expression level of IL‐10 anti‐inflammatory factors (Gao et al., 2016; Holditch et al., 2019). Therefore, we used the inflammatory experiment of RAW 264.7 cell induced by LPS to confirm whether ONP had anti‐inflammatory activity in vitro (Hwang et al., 2020).…”

Section: Resultsmentioning

confidence: 99%

“…The inflammatory status was induced by LPS in RAW 264.7 cells. It is known that LPS stimulates macrophages to express more nitric oxide synthase (iNOS) and cyclooxygenase‐2 (COX‐2) to produce NO and prostaglandin E2 (Hwang et al., 2020)[20]. Moreover, iNOS and COX‐2 activate the expression levels of several pro‐inflammatory mediators, such as TNF‐α and IL‐1β.…”

Section: Discussionmentioning

confidence: 99%

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A neutral polysaccharide from Ophiocordyceps lanpingensis restrains cisplatin‐induced nephrotoxicity

Zhou

et al. 2021

Food Science & Nutrition

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Ophiocordyceps lanpingensis is an edible mushroom distributed over the south‐eastern part of the Tibet Plateau, which is also recognized as an effective ethnomedicine to alleviate diseases. This study explored the effects of a kind of Ophiocordyceps lanpingensis neutral polysaccharide (ONP) on RAW264.7 macrophages and cisplatin‐induced nephrotoxicity. The results showed that ONP relieved the inflammatory response of RAW264.7 macrophages by increasing the expression level of anti‐inflammatory factor IL‐10. Furthermore, ONP treatment significantly prolonged the survival of the mice treated by cisplatin through decelerating pathological progress and alleviating damaged functions of the kidneys. Compared with the cisplatin group, ONP reduced the oxidative stress of the renal cells and the expression levels of pro‐inflammatory factors. Apoptosis of renal cells was also weakened in the ONP treatment group. These findings indicated that ONP alleviated cisplatin nephrotoxicity mainly by inhibiting oxidative stress, inflammation, and apoptosis in the kidneys, underscoring the potential of ONP supplementation to alleviate the side effects of cisplatin chemotherapy.

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“…The LPS-induced macrophage RAW264.7 inflammatory model is commonly used to detect the anti-inflammatory activity of natural drugs [28,29]. RWA264.7 cells are also being used to research gastrointestinal mucosal damage and anti-inflammation mechanisms [30,31]. LPS, an endotoxin, transmits stimulation signals to cells by binding to intracellular functional LPS transmembrane receptors (Toll-like receptors), activating macrophages that cause the release of inflammatory mediators NO, TNF-α, and IL-1β and leading to inflammation and tissue damage [32].…”

Section: Discussionmentioning

confidence: 99%

Anti-Inflammation and Protective Effects of Anethum graveolens L. (Dill Seeds) on Esophageal Mucosa Damages in Reflux Esophagitis-Induced Rats

Nam

Choo

2021

Foods

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Anethum graveolens L. (dill seeds) are important medicinal and functional foods in Europe and central and south Asia, often used as a seasoning in daily diets. Anethum graveolens L. seeds (AGS) are used to treat indigestion and have shown physiological activities such as those against hypoglycemia and gastroesophageal disease. This study explored the protective effects of AGS extract on mucosal damages and inflammation in reflux esophagitis rats. AGS inhibited cellular inflammation including NO production and the expression of inflammatory proteins (iNOS and COX2 etc.), cytokines (IL-1β and TNF-α) and nuclear transfer factor related to NF-κB signaling caused by LPS stimulation in vitro. Furthermore, reflux esophagitis-induced rats were used to observe the anti-inflammatory effect of AGS. Tissue staining and inflammation-related protein expression of rats with acute reflux esophagitis indicated that AGS improved this inflammatory response, such as COX-2 and TNF-α in mucosa. In conclusion, AGS have good physiological activity and the possibility of being used as a medicinal food and a functional resource for the prevention and therapy of gastroesophageal diseases.

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Anti-Inflammatory Effect of Immature Sword Bean Pod (Canavalia gladiata) in Lipopolysaccharide-Induced RAW264.7 Cells

Cited by 11 publications

References 40 publications

Immature sword bean pods (Canavalia gladiata) inhibit adipogenesis in C3H10T1/2 cells and mice with high-fat diet–induced obesity

Immature sword bean pods (Canavalia gladiata) inhibit adipogenesis in C3H10T1/2 cells and mice with high-fat diet–induced obesity

A neutral polysaccharide from Ophiocordyceps lanpingensis restrains cisplatin‐induced nephrotoxicity

Anti-Inflammation and Protective Effects of Anethum graveolens L. (Dill Seeds) on Esophageal Mucosa Damages in Reflux Esophagitis-Induced Rats

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