Anti-inflammatory effect of ozonated krill (Euphausia superba) oil in lipopolysaccharide-stimulated RAW 264.7 macrophages

Kim, Hong-Deok; Lee, Soo Bin; Ko, Seok‐Chun; Jung, Won‐Kyo; Kim, Young‐Mog; Kim, Seon-Bong

doi:10.1186/s41240-018-0092-1

Cited by 11 publications

(6 citation statements)

References 38 publications

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“…Similar results were also found by Costanzo et al, who reported that the mRNA expression of IL-1β, IL-6 and IL-10 was decreased after administration with krill oil in C57BL/6 mice-induced colitis with dextran sodium sulphate (DSS) [45]. Ozonated krill oil also showed a reduction effect on the expression of the proinflammatory cytokines IL-1β and IL-6 at the mRNA expression level in LPS-induced RAW264.7 [32]. However, these studies did not provide the chemical compositions of the krill oil used.…”

Section: Effect Of Three Krill Lipid Fractions On Cytokine Release and Related Gene Expression In Raw2647 Cells With Lps Stimulationsupporting

confidence: 87%

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Diverse Krill Lipid Fractions Differentially Reduce LPS-Induced Inflammatory Markers in RAW264.7 Macrophages In Vitro

Xie

Fangyuan

Wang³

et al. 2021

Foods

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Antarctic krill oil is an emerging marine lipid and expected to be a potential functional food due to its diverse nutrients, such as eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), phospholipids, astaxanthin and tocopherols. Although krill oil has been previously proved to have anti-inflammatory activity, there is little information about the relationship between its chemical compositions and anti-inflammatory activity. In this study, the RAW264.7 macrophages model was used to elucidate and compare the anti-inflammatory potential of different krill lipid fractions: KLF-A, KLF-H and KLF-E, which have increasing phospholipids, EPA and DHA contents but decreasing astaxanthin and tocopherols levels. Results showed that all the krill lipid fractions alleviated the inflammatory reaction by inhibition of production of nitric oxide (NO), release of tumor necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-6 and gene expression of proinflammatory mediators including TNF-α, IL-1β, IL-6, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS). In addition, KLF-E with the highest phospholipids, EPA and DHA contents showed the strongest inhibition effect on the LPS-induced proinflammatory mediator release and their gene expressions. The results would be helpful to provide powerful insights into the underlying anti-inflammatory mechanism of krill lipid and guiding the production of krill oil products with tailor-made anti-inflammatory activity.

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Section: Effect Of Three Krill Lipid Fractions On Cytokine Release and Related Gene Expression In Raw2647 Cells With Lps Stimulationsupporting

confidence: 87%

“…The obtained krill lipid fraction was dissolved in DMSO with 2% tween 80 as reported by Kim et al [32], and then the mixture was homogenized using an Ultra Turrax T25 blender (IKA, Staufen, Germany) at 12,000 rpm for 5 min. The mixture was used for cell experiments.…”

Section: Preparation and Analysis Of Three Krill Lipid Fractions With Different Compositionsmentioning

confidence: 99%

Diverse Krill Lipid Fractions Differentially Reduce LPS-Induced Inflammatory Markers in RAW264.7 Macrophages In Vitro

Xie

Fangyuan

Wang³

et al. 2021

Foods

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show abstract

“…KO also contains biologically active components, such as astaxanthin and vitamins A and E [ 21 ]. Previous studies have reported that these KO components have various physiological effects, such as anti-hyperlipidemic [ 22 ], anti-inflammatory [ 23 ], anti-oxidative stress [ 24 ], anti-arthritis [ 25 ], and neurocognitive improvement [ 26 ]. In particular, the anti-obesity effects have been demonstrated to reduce weight gain and improve serum lipid levels in several animal and clinical trials utilizing krill oil supplements [ 22 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 ].…”

Section: Introductionmentioning

confidence: 99%

“…In particular, the anti-obesity effects have been demonstrated to reduce weight gain and improve serum lipid levels in several animal and clinical trials utilizing krill oil supplements [ 22 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 ]. KO suppressed the expression of proinflammatory mediators and cytokines by inhibiting the phosphorylation of p38 mitogen-activated protein kinase (MAPK) and c-Jun N-terminal kinase (JNK) in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

Partial Replacement of Dietary Fat with Krill Oil or Coconut Oil Alleviates Dyslipidemia by Partly Modulating Lipid Metabolism in Lipopolysaccharide-Injected Rats on a High-Fat Diet

Son

Kim

Lee

et al. 2022

IJERPH

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This study investigated the effects of partial replacement of dietary fat with krill oil (KO) or coconut oil (CO) on dyslipidemia and lipid metabolism in rats fed with a high-fat diet (HFD). Sprague Dawley rats were divided into three groups as follows: HFD, HFD + KO, and HFD + CO. The rats were fed each diet for 10 weeks and then intraperitoneally injected with phosphate-buffered saline (PBS) or lipopolysaccharide (LPS) (1 mg/kg). The KO- and CO-fed rats exhibited lower levels of serum lipids and aspartate aminotransferases than those of the HFD-fed rats. Rats fed with HFD + KO displayed significantly lower hepatic histological scores and hepatic triglyceride (TG) content than rats fed with HFD. The KO supplementation also downregulated the adipogenic gene expression in the liver. When treated with LPS, the HFD + KO and HFD + CO groups reduced the adipocyte size in the epididymal white adipose tissues (EAT) relative to the HFD group. These results suggest that KO and CO could improve lipid metabolism dysfunction.

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“…In the last few years, a renewed interest in the therapeutic potential of ozone has emerged. In particular, the focus is on the ability to promote wound healing, to attenuate the adverse effects of inflammation by reducing the oxidative activities of inflammatory cells, to express antimicrobial activities against various bacterial species and mycetes pathogenic for humans, and also against biofilm-producing and antibiotic-resistant bacteria, thus offering chances of overcoming antibiotic-resistance issues [30][31][32][33][34][35][36][37][38][39] (Figure 2).…”

Section: Discussionmentioning

confidence: 99%

Ozone Gel in Chronic Periodontal Disease: A Randomized Clinical Trial on the Anti-Inflammatory Effects of Ozone Application

Colombo

Gallo

Garofoli

et al. 2021

Biology

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The search for new topical treatments able to display not only antimicrobial properties but also a multiplicity of other beneficial effects while expressing safe cytocompatibility toward host tissues is being progressively developed. Antiseptics represent an aid to the gold standard nonsurgical treatment Scaling-and-Root-Planing (SRP) for periodontal disease. This split-mouth study aims to assess the efficacy of the ozonized gel GeliO3 (Bioemmei Srl, Vicenza, Italy) plus SRP (experimental treatment), with respect to SRP + chlorhexidine gel. Ten participants were treated with SRP + chlorhexidine gel (control sites) and with SRP + ozone gel (trial sites). After 1 (T1) and 3 months (T2) from baseline (T0), patients were revisited. At each time-point, the following indexes were assessed: probing pocket depth (PPD), clinical attachment level (CAL), gingival index (GI), plaque index (PI), and bleeding on probing (BoP). It has been assessed that the use of the ozonized gel in addition to SRP did not show significant differences if compared to conventional SRP + chlorhexidine. Chlorhexidine was found to be more effective than ozone in reducing CAL and GI at T2. Ozone deserves consideration for its wide applicability in several clinical fields. In this connection, we also glance at the latest research on ozone therapy.

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Anti-inflammatory effect of ozonated krill (Euphausia superba) oil in lipopolysaccharide-stimulated RAW 264.7 macrophages

Cited by 11 publications

References 38 publications

Diverse Krill Lipid Fractions Differentially Reduce LPS-Induced Inflammatory Markers in RAW264.7 Macrophages In Vitro

Diverse Krill Lipid Fractions Differentially Reduce LPS-Induced Inflammatory Markers in RAW264.7 Macrophages In Vitro

Partial Replacement of Dietary Fat with Krill Oil or Coconut Oil Alleviates Dyslipidemia by Partly Modulating Lipid Metabolism in Lipopolysaccharide-Injected Rats on a High-Fat Diet

Ozone Gel in Chronic Periodontal Disease: A Randomized Clinical Trial on the Anti-Inflammatory Effects of Ozone Application

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