Anti-inflammatory Effect of Quercetin on Picryl Chloride-induced Contact Dermatitis in BALB/c Mice

Abstract

In this study, we investigated that anti-inflammatory effect of quercetin on picryl chloride(PCL)-induced contact dermatitis in BALB/c Mice. Experimental animals were divided into three groups and comprising five animals. All groups of oral administration was begun on the first day of PCL treatment and ceased on day 5. For the induction of contact dermatitis, BALB/c mice were sensitized with 40 µL of 1.5% picryl choloride (PCL) to the left and right ear, respectively. Ear swelling responses were much weaker in highdose group (100 mg/kg) than control group (0 mg/kg). Total serum IgE levels and histamine levels were measured by sandwich ELISA method using mouse IgE, histamine measuring Kit. Both total serum IgE and histamine levels were significantly decreased in high-dose group (100 mg/kg) than other groups. Degranulation of mast cells were also confirmed by Toluidine Blue (TB) staining method. In high-dose group (100 mg/kg), the number of mast cells were significantly decreased and there are many mast cells were shown degranulation in control group (0 mg/kg). All of these results demonstrate that the pharmacological actions of quercetin indicate their potential activity for allergic inflammatory diseases through the down-regulation of mast cell activation.

Diosgenin effectively suppresses skin inflammation induced by phthalic anhydride in IL-4/Luc/CNS-1 transgenic mice

“…In vivo imaging analysis to detect luciferase-derived signal was conducted using an IVIS imaging system (Xenogen, Oakland, CA, USA) as previously described. 17) Briefly, IL-4/Luc/CNS-1 Tg mice were anesthetized with Zoletil and injected i.p. with 150 mg/kg of D-luciferin (Sigma-Aldrich Co.).…”

Characterization of Changes in Global Genes Expression in the Distal Colon of Loperamide-Induced Constipation SD Rats in Response to the Laxative Effects of Liriope platyphylla

“…L . platyphylla is known to potently inhibit airway inflammation and hyper-responsiveness in a murine model of asthma by modulating the relationship between Th1/Th2 cytokine imbalance [ 12 ], atopic dermatitis induced by phthalic anhydride treatment [ 13 , 14 ], and act effectively on the process of obesity and diabetes [ 15 , 16 ]. In addition, significant alteration of nerve growth factor (NGF) secretion as well as its related signaling pathway has been observed in the extracts and compounds isolated from L .…”

Laxative effects ofLiriope platyphyllaare tightly correlated with suppression of endoplasmic reticulum stress in loperamide-induced constipation of SD rats

“…Next, 600 g of dry roots were reduced to powder using an electric blender, then extracted in 2 L of distilled water, after which the water extract was purified at 100℃ for 2 h using circulating extraction equipment (IKA Labortechnik, Staufen, Germany). The calories and main composition of AEtLP were measured in previous studies [ 15 16 ]. Extract solutions were concentrated into dry pellets using a rotary evaporator (EYELA, Tokyo, Japan), then stored at -80℃ until use.…”