Anti-inflammatory Effects of Amelanchier asiatica Fruits Ethanol Extract

Lee, Jin Young; Yoo, Dan-Hee; 주다혜,; 김소라,; ), 조희선 ( Hui Seon Jo; Joo, Sung Hyun; Chae, Jung-Woo

doi:10.15230/scsk.2017.43.1.19

Cited by 5 publications

(2 citation statements)

References 13 publications

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“…NO, an inflammatory mediator produced by activating RAW264.7 cells with LPS, was measured using Griess reagent in the form of nitrite ions (NO 2− ) in the culture medium (Figure 4) [12]. At 1 day, NO production was suppressed in a concentration-dependent manner, and at a high concentration of 250 µg/mL, 36% was suppressed.…”

Section: Measurement Of Nitric Oxide Productionmentioning

confidence: 99%

Anti-Inflammatory Effects and Their Correlation with Microbial Community of Shindari, a Traditional Jeju Beverage

Hyun

2020

Fermentation

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In this study, we investigated the microbial community and its correlation with anti-inflammatory effects during the fermentation procedure of shindari. Since shindari is traditionally manufactured through a fermentation process of 3–4 days in summer or 5–6 days in winter, the shindari materials applied in this study were fermented for 1, 3, and 5 days, respectively. Microbial community structure in the shindari manufacturing process was analyzed by Illumina MiSeq sequencing. Results revealed different fermentation time had different influences on the community structure and microbial diversity in the shindari process. Ultimately, we found that pathogenic bacteria such as Enterococcus durans (9.3%) and Enterobacter asburiae (4.5%) dominate the shindari fermented for 1 day, while samples with a fermentation period of more than 3 days occupied lactic acid bacteria such as Pediococcus sp. (97.1%). In addition, to study the relationship between fermentation period and anti-inflammatory effect in the shindari manufacturing process, we applied RAW264.7 cells, a classic cell model for inflammation studies. First, we prepared an ethyl acetate extract of shindari fermented for 1 (S1), 3 (S3), or 5 days (S5), and then it was confirmed that all of these extracts inhibited the nitric oxide (NO) production in a concentration-dependent manner. In addition, these inhibitory effects were correlated with the suppressive effect of shindari extracts against overexpression of inducible nitric oxide synthase (iNOS). Furthermore, S3 and S5 also inhibited the production of pro-inflammatory cytokines such as tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and IL-6. Overall, the anti-inflammatory effect of S3 was suggested to be mediated through the negative regulation of mitogen-activated protein kinase signaling (MAPKs) and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signaling pathways.

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Section: Measurement Of Nitric Oxide Productionmentioning

confidence: 99%

Anti-Inflammatory Effects and Their Correlation with Microbial Community of Shindari, a Traditional Jeju Beverage

Hyun

2020

Fermentation

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“…최근 현대사회에서는 경제, 과학, 의학 기술 등의 발전으 로 인해 평균 수명이 연장되어 고령화 사회에 접어들었다. 또한 도시화와 산업화가 중심이 되며 생물학적, 화학적 유해 물질에 대한 노출이 빈번해지고 환경오염, 주거환경 변화, 식 생활 변화 등에 의해 생체내의 면역 조절 이상이 나타나게 되어 아토피, 알러지 및 염증 반응 등의 염증성 질환 발병이 증가하고 있다 [14]. 이로 인해 최근 현대인들에게 많이 발생 되는 염증성 질환에 대한 예방 및 치료를 위해 염증 매개 물 질들을 억제할 수 있는 천연 유래 소재를 활용하여 항염증 활성 억제에 관한 연구들이 활발히 진행되고 있다 [15].…”

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Verification of Anti-Inflammatory Efficacy of Apple Mango (Mangifera indica L.) Peel in LPS-Activated Macrophage

Kim

Yoo

Lee

2022

Microbiol. Biotechnol. Lett.

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The purpose of this study was to investigate the antioxidant and anti-inflammatory activities of hot water (AMPW) and 70% ethanol (AMPE) extracts of apple mango (Mangifera indica L.) peel. The antioxidant activities were measured using a total polyphenol, electron-donating, 2,2'-azinobis [3-ethylbenzothiazoline-6-sulfonic acid] (ABTS) radical scavenging assay. The total polyphenol content of AMPW and AMPE was 66.08 ± 0.62 mg TAE/100 g and 100.13 ± 0.23 mg TAE/100 g, respectively. As a result of measuring the electrondonating ability, at a concentration of 1,000 µg/ml, AMPW and AMPE showed an effectiveness of 86% and 94%, respectively. The ABTS assay showed 80% and 98% respective radical scavenging activity for AMPW and AMPE, at a concentration of 1,000 µg/ml. The cell viability on macrophage cells was performed using a 3-[4,5-dimethyl-thiazol-2-yl]-2,5-diphenyl-tetrazoliumbromide (MTT) assay, and the results showed more than 90% cell viability at a 100 µg/ml concentration. Anti-inflammatory activity was verified by confirming nitric oxide (NO) production inhibitory activity, inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) protein and mRNA expression inhibitory activity from lipopolysaccharide (LPS)-treated RAW 264.7 cells. The NO production inhibitory effects were measured using the Griess assay, which confirmed 45% and 40% inhibition after treatment with AMPW and AMPE, respectively. Moreover, the protein and mRNA expression of inflammatory-related factors iNOS and COX-2, decreased in a concentrationdependent manner. In conclusion, this study showed antioxidant and anti-inflammatory effects of Mangifera indica L. peel and revealed its promising potential for application as an antioxidant and anti-inflammatory agent.

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Anti-oxidative and Anti-inflammatory Effects of Codonopsis lanceolata Skin Extracts

Jung¹,

Ryu²

2018

Asian J Beauty Cosmetol

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Purpose: The aim of this study was to investigate the biological and cosmetic properties and industrial value of Codonopsis lanceolata (C. lanceolata, Deodeok) skin extract. Methods: 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity, total polyphenol content, flavonoid content and superoxide dismutase (SOD)like activity were measured to investigate the anti-oxidative effect of C. lanceolata skin extract, while its cytoprotective effect was studied on HaCaT cells treated with hydrogen peroxide. Nitric oxide (NO) production inhibition was evaluated using Griess' method, after inducing an inflammatory response in RAW 264.7 cells using lipopolysaccharides (LPS). Results: DPPH radical-scavenging ability at 500 μg/ mL was 74.93% and 85.25% for hot water and ethanol extracts, respectively, while total polyphenol contents were 70.22 and 75.27 μg/mL and total flavonoid contents were 16.12 and 22.65 μg/mL; SOD-like activities of the extracts were 62.2% and 69.93% at 500 μg/mL. The extracts exhibited a 30% and 36% protective effect at 100 μg/mL on hydrogen peroxide-treated HaCaT keratinocytes, respectively, and NO inhibition in RAW 264.7 cells induced with LPS was 14.57 and 11.45 μM at 100 μg/mL concentration. Conclusion: These results suggest that the ethanol extract of C. lanceolata skin has higher anti-oxidative, cytoprotective, and anti-inflammatory effects than the hot water extract, and is a candidate for a natural, functional cosmetic material.

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Anti-inflammatory Effects of Amelanchier asiatica Fruits Ethanol Extract

Cited by 5 publications

References 13 publications

Anti-Inflammatory Effects and Their Correlation with Microbial Community of Shindari, a Traditional Jeju Beverage

Anti-Inflammatory Effects and Their Correlation with Microbial Community of Shindari, a Traditional Jeju Beverage

Verification of Anti-Inflammatory Efficacy of Apple Mango (Mangifera indica L.) Peel in LPS-Activated Macrophage

Anti-oxidative and Anti-inflammatory Effects of Codonopsis lanceolata Skin Extracts

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