Anti-inflammatory Effects of Cnidium Rhizoma against Intracerebral Hemorrhage in Rats

Abstract

Objectives : Inflammation is mediated by cellular components, such as leukocytes and microglia, and molecular components, including cytokines, extracellular proteases, and reactive oxygen species. Cnidium Rhizoma effects the anti-inflammatory, antioxidant, suppression of the microglia activation and protection of the nerve cell injury. For this reason, we investigated the anti-inflammatory effects of water extracts of Cnidium Rhizoma on intracerebral hemorrhage (ICH). Method : ICH was induced by the stereotaxic intracerebral injection of bacterial collagenase type IV (0.23 U/㎕, 0.1 ㎕/min) in Sprague-Dawley rats. We orally administrated once 3 hours after ICH, then 2 times at 24-hour intervals the water extracts of Cnidium Rhizoma (500 ㎎/㎏), myeloperoxidase (MPO) was observed by using immunofluorescense and expression of inducible nitric oxide synthase (iNOS), tumor necrosis factor-α (TNF-α ) and microglia were observed by using immunohistochemistry. Results : Infiltration of MPO expressing neutrophil, expression of iNOS and TNF-α and activated microglia were significantly reduced in peri-hematoma of the rats fed with water extracts of Cnidium Rhizoma. Conclusion : These results demonstrated that water extracts of Cnidium Rhizoma suppressed an inflammatory reaction through inhibition of MPO, iNOS and TNF-α positive cell and activated microglia number in peri-hematoma of ICH-induced rats.

Anti-inflammatory Effects of Naetakbaekryeom-san

“…The anti-inflammatory efficacy of individual herbs in NTB Paeoniae Radix 12) , Angelicae Gigantis Radix 13) , Forsythiae Fructus 14) , Angelicae Dahuricae Radix 15) , Ampelopsis Radix 16) , Scutellariae Radix 17) , Trichosanthis Fructus 18) , Cnidii Rhizoma 19) , Trichosanthis Radix 20) , Olibanum 21) , Bupleuri Radix 22) , Saposhnikoviae Radix 23) , Platycodi Radix 24) , Tribuli Fructus 25) , and Glycyrrhizae Radix 26) has been reported, but the anti-inflammatory effects of NTB have not been studied, Therefore we studied the anti-inflammatory effects of NTB.…”

“…Naetakbaekryeom-san (NTB) is a traditional Korean herbal medicine described in Man-Byeong-Hoi-Chun, that is composed of Paeoniae Radix, Angelicae Gigantis Radix, Forsythiae Fructus, Angelicae Dahuricae Radix, Ampelopsis Radix, Trichosanthis Fructus, Scutellariae Radix, Cnidii Rhizoma, Trichosanthis Radix, Olibanum, Saposhnikoviae Radix, Platycodi Radix, Bupleuri Radix, Tribuli Fructus, and Glycyrrhizae Radix 10,11) . Although studies have reported the anti-inflammatory effects of each of these herbs [12][13][14][15][16][17][18][19][20][21][22][23][24][25][26] , the anti-inflammatory effects of NTB, a complex herbal medicine, have not been studied. Therefore, we conducted a study on the anti-inflammatory efficacy of NTB water extract to determine whether NTB could have therapeutic potential for the treatment of inflammation.…”