Anti-Inflammatory Effects of Concentrated Ethanol Extracts of Edelweiss (Leontopodium alpinumCass.) Callus Cultures towards Human Keratinocytes and Endothelial Cells

Abstract

Edelweiss (Leontopodium alpinum Cass.) is traditionally employed in folk medicine as an anti-inflammatory remedy. In nature, the plant is sparsely available and protected; therefore production of callus cultures was established. A concentrated ethanolic extract of culture homogenate, with leontopodic acid representing 55 ± 2% of the total phenolic fraction (ECC55), was characterized for anti-inflammatory properties in primary human keratinocytes (PHKs) and endotheliocytes (HUVECs). Inflammatory responses were induced by UVA+UVB, lipopolysaccharide (LPS), oxidized low-density lipoprotein (oxLDL), and a mixture of proinflammatory cytokines. Trichostatin A, a sirtuin inhibitor, was used to induce keratinocyte inflammatory senescence. ECC55 (10–50 μg/mL) protected PHK from solar UV-driven damage, by enhancing early intracellular levels of nitric oxide, although not affecting UV-induced expression of inflammatory genes. Comparison of the dose-dependent inhibition of chemokine (IL-8, IP-10, MCP-1) and growth factor (GM-CSF) release from PHK activated by TNFα + IFNγ showed that leontopodic acid was mainly responsible for the inhibitory effects of ECC55. Sirtuin-inhibited cell cycle, proliferation, and apoptosis markers were restored by ECC55. The extract inhibited LPS-induced IL-6 and VCAM1 genes in HUVEC, as well as oxLDL-induced selective VCAM1 overexpression. Conclusion. Edelweiss cell cultures could be a valuable source of anti-inflammatory substances potentially applicable for chronic inflammatory skin diseases and bacterial and atherogenic inflammation.

Secondary Plant Metabolites for Sun Protective Cosmetics: From Pre-Selection to Product Formulation

“…Theoretically, SPMs could modify skin-UV interaction at several crucial points: (a) by absorption of UVA + UVB (screen action); (b) by inhibition of UV-induced free radical reactions in skin cells and extracellular matrix (scavenging and direct antioxidant chain-breaking effects); (c) by protection of skin surface lipid antioxidants, such as alpha-tocopherol, coenzyme Q10, and squalene (antioxidant rescue action); (d) by induction of endogenous antioxidant systems in keratinocytes and fibroblasts (indirect antioxidant effects); (e) by attenuation of inflammatory responses in cutaneous immune cells (keratinocytes/leukocytes/dendritic cells); (f) by modulation of excessive metabolic and proliferative UV-induced stress responses (anti-stress effects), and (g) by attenuation of UV-related immune suppression (immuno-modulation) [23,[78][79][80].…”

Meristem Plant Cells as a Sustainable Source of Redox Actives for Skin Rejuvenation

“…Ex Stocks)) seems to have perspectives in the development of cosmeceutical treatments for acne vulgaris [159]. Anti-replicative senescence effects via sirtuin-dependent mechanisms have been reported for meristem cells of Leontopodium alpinum L. and Lippia citrobara L. [30,160]. The topical application of meristem cells from Syringa vulgaris provided skin healing and UV-protection [2,37,41,106] as well as anti-inflammatory and anti-microbial effects [30,41].…”

Anti-Aging Effects of Leontopodium alpinum (Edelweiss) Callus Culture Extract through Transcriptome Profiling

“…For a long time, edelweiss has been used as traditional medicine against abdominal aches, bronchitis, diarrhea, dysentery, and fever [3,4]. Recently, several studies have shown the efficacy of edelweiss extracts for anti-inflammation in mice and rats [3] and human keratinocytes and endothelial cells [4].…”