Anti-inflammatory effects of ivermectin in mouse model of allergic asthma

Yan, Shuhan; Ci, Xinxin; Chen, Na; Chen, Chi; Li, Xiangchao; Chu, Xiao; Li, Jianhua; Deng, Xuming

doi:10.1007/s00011-011-0307-8

Cited by 103 publications

(74 citation statements)

References 36 publications

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“…Proinflammatory cytokines, including IL-4, IL-5 and IL-13, expressed by Th2 cells are directly involved in the initiation and maintenance of allergic disease (27). IL-4 may be responsible for the production of IgE (28). IL-5 promotes the mobilization of eosinophils from the bone marrow, and recruits eosinophils into the airways (29).…”

Section: Discussionmentioning

confidence: 99%

Anti-inflammatory effects of dihydromyricetin in a mouse model of asthma

Huang

Wang

et al. 2017

Molecular Medicine Reports

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Dihydromyricetin (DHM) is a plant flavonoid and is the primary active ingredient isolated from the medicinal herb, Ampelopsis grossedentata. DHM has been shown to possess various pharmacological activities, including anti-inflammatory effects. However, the possible role of DHM in asthma treatment remains to be elucidated. The present study aimed to investigate its anti-inflammatory properties in mice with symptoms of allergic asthma. The C57BL/6 mice were sensitized and challenged with ovalbumin (OVA) to induce asthma. DHM or phosphate-buffered saline treatment was administered 1 h prior to the OVA challenge. The levels of interleukin (IL)-4, IL-5 and IL-13 in the bronchoalveolar lavage (BAL) fluid were measured by enzyme-linked immunosorbent assay (ELISA), and OVA-specific serum IgE and IgG1 levels were also determined by ELISA. Histopathological staining was performed to evaluate the infiltration of inflammatory cells into the BAL fluid, lung tissues and goblet cell hyperplasia. DHM treatment significantly reduced the total number of inflammatory cells, including eosinophils, neutrophils, lymphocytes and macrophages, in the BAL fluid. DHM also reduced the levels of IL-4, IL-5 and IL-13 in the BAL fluid, and reduced the secretion of OVA-specific IgE and IgG1 in the serum. The histological staining demonstrated that DHM treatment effectively suppressed the OVA-induced inflammatory cells in the lung tissues and in the mucus hypersecreted by goblet cells in the airway. These results showed that DHM had a potent anti-inflammatory effect in an OVA-induced mouse model of asthma, offering potential as an anti-inflammatory agent for the treatment of asthma.

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Section: Discussionmentioning

confidence: 99%

Anti-inflammatory effects of dihydromyricetin in a mouse model of asthma

Huang

Wang

et al. 2017

Molecular Medicine Reports

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“…Work performed in with a murine asthma model suggested that ivermectin might possess anti-inflammatory effects (Yan et al, 2011). We recently tested this hypothesis by simultaneously vaccinating and treating ponies with either pyrantel pamoate, ivermectin, or no anthelmintic treatment (Nielsen et al, 2015), and Local gene expression of pro-and anti-inflammatory cytokines interferon ␥ (IFN-␥), tumor necrosis factor ␣ (TNF-␣), transforming growth factor ␤ (TGF-␤), interleukin 1␤ (IL-1␤), interleukin 4 (IL-4), interleukin 5 (IL-5), interleukin 6 (IL-6), and interleukin 10 (IL-10) given in mean relative quantity (RQ) for each group (n = 6) and presented by cecum (gray columns) and ventral colon (black columns).…”

Section: Discussionmentioning

confidence: 99%

Local and systemic inflammatory and immunologic reactions to cyathostomin larvicidal therapy in horses

Nielsen

Loynachan

Jacobsen

et al. 2015

Veterinary Immunology and Immunopathology

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“…Subsequently, lavaged samples were centrifuged at 400 g for 5 min at 4°C and the cells pellets were resuspended in 500 µL of PBS for analysis of a total number of inflammatory cells in BALF with a hemocytometer. Smears of BALF cells were performed as described previously [15] and stained with a modified Wright's staining for the differential cell counts that were performed on at least 300 cells in each slide according to standard morphological criteria.…”

Section: Methodsmentioning

confidence: 99%

Bufalin Inhibits the Inflammatory Effects in Asthmatic Mice through the Suppression of Nuclear Factor-Kappa B Activity

Zhakeer¹,

Hadeer²,

Tuerxun

et al. 2017

Pharmacology

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Asthma is an inflammatory airway disease characterized by increased infiltration of inflammatory cells into the airways and poor respiratory function. Bufalin is one of the biological ingredients obtained from Chansu. Bufalin was found to possess various pharmacological properties including anti-inflammatory activities. However, the effect of bufalin treatment on asthma has not yet been reported. Therefore, this study aimed to investigate the inhibitory effect of bufalin on asthmatic response in a murine model. A mouse asthma model was developed by ovalbumin (OVA) sensitization and challenge in the BALB/c mice. OVA-specific serum IgE and the levels of interleukin (IL)-4, IL-5, and IL-13 in bronchoalveolar lavage fluid (BALF) were determined by an enzyme-linked immunosorbent assay. Recruitment of inflammatory cells into BALF or lung tissues, and goblet cell hyperplasia were evaluated by histological staining. The expression levels of inhibitory subunit of nuclear factor-kappa B (NF-κB) alpha (IκBα) and phosphorylated p65 protein were measured by Western blot analyses. The results demonstrated that bufalin (5 and 10 mg/kg) markedly attenuated hyperresponsiveness, and strongly suppressed the OVA-induced increases of total inflammatory cells including macrophages, eosinophils, lymphocytes, and neutrophils in BALF. The levels of IL-4, IL-5, and IL-13 in BALF and OVA-specific IgE in serum were significantly reduced by bufalin. Histological staining of lung tissues showed that bufalin reduced inflammatory cell infiltration and goblet cell hyperplasia. The results of Western blotting indicated that bufalin suppressed the IκBα degradation from NF-κB, and reduced the level of phosphorylated p65 protein in the lung tissues. These data suggest that bufalin can exert its anti-inflammatory effects possibly through the inhibition of the NF-κB activity.

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Anti-inflammatory effects of ivermectin in mouse model of allergic asthma

Abstract: This is the first study to demonstrate that ivermectin is an effective suppressor of inflammation and may be efficacious in the treatment of non-infectious airway inflammatory diseases such as allergic asthma.

Cited by 103 publications

References 36 publications

Anti-inflammatory effects of dihydromyricetin in a mouse model of asthma

Anti-inflammatory effects of dihydromyricetin in a mouse model of asthma

Local and systemic inflammatory and immunologic reactions to cyathostomin larvicidal therapy in horses

Bufalin Inhibits the Inflammatory Effects in Asthmatic Mice through the Suppression of Nuclear Factor-Kappa B Activity

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