Anti‐inflammatory effects of liquiritigenin as a consequence of the inhibition of NF‐κB‐dependent iNOS and proinflammatory cytokines production

Kim, Y W; Zhao, Rongjie; Park, S J; Lee, J R; Cho, I J; Yang, Chae Ha; Kim, S G; Kim, S C

doi:10.1038/bjp.2008.79

Cited by 224 publications

(159 citation statements)

References 46 publications

Supporting

Mentioning

144

Contrasting

Unclassified

Order By: Relevance

“…In a previous study, Kim et al showed that liquiritigenin exerted anti-inflammatory effects against lipopolysaccharide in Raw264.7 cells at a concentration ranging from 3 to 30 μmol/L [18] . We reckon that the differences in effective dose observed in the two studies may be due to the use of different cells and/or different toxic factors.…”

Section: Discussionmentioning

confidence: 99%

“…ERβ is expressed in brain centers related to learning and memory and is less likely than ERα to be related to sexual function [14,15] . Second, liquiritigenin has been shown in several studies to exert cytoprotective effects [16,17] ; recent studies demonstrate that liquiritigenin exerts anti-inflammatory effects through the NF-κB pathway [18] . Third, we previously observed that liquiritigenin did not induce proliferation of MCF-7 and T47D breast cancer cells (data not shown), which is consistent with other studies [13] .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Liquiritigenin inhibits Aβ25–35-induced neurotoxicity and secretion of Aβ1–40 in rat hippocampal neurons

2009

View full text Add to dashboard Cite

Aim: To examine whether liquiritigenin, a newly found agonist of selective estrogen receptor-β, has neuroprotective activity against β-amyloid peptide (Aβ) in rat hippocampal neurons. Methods: Primary cultures of rat hippocampal neurons were pretreated with liquiritigenin (0.02, 0.2, and 2 μmol/L) prior to Aβ 25-35 exposure. Following treatment, viability of the cells was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide analysis and by a lactate dehydrogenase activity-based cytotoxicity assay. Intracellular Ca 2+ concentration ([Ca 2+ ] i ) and levels of reactive oxygen species (ROS), as well as apoptotic rates, were determined. Our studies were extended in tests of whether liquiritigenin treatment could inhibit the secretion of Aβ 1-40 as measured using an ELISA method. In order to analyze which genes may be involved, we used a microarray assay to compare gene expression patterns. Finally, the levels of specific proteins related to neurotrophy and neurodenegeration were detected by Western blotting. Results: Pretreated neurons with liquiritigenin in the presence of Aβ 25-35 increased cell viability in a concentration-dependent manner. Liquiritigenin treatment also attenuated Aβ 25-35 -induced increases in [Ca 2+ ] i and ROS level and decreased the apoptotic rate of neurons. Some genes, including B-cell lymphoma/leukemia-2 (Bcl-2), neurotrophin 3 (Ntf-3) and amyloid β (A4) precursor protein-binding, family B, member 1 (Apbb-1) were regulated by liquiritigenin; similar results were shown at the protein level by Western blotting. Conclusion: Our results demonstrate that liquiritigenin exhibits neuroprotective effects against Aβ 25-35 -induced neurotoxicity and that it can decrease the secretion of Aβ 1-40 . Therefore, liquiritigenin may be useful for further study as a prodrug for treatment of Alzheimer's disease.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Liquiritigenin inhibits Aβ25–35-induced neurotoxicity and secretion of Aβ1–40 in rat hippocampal neurons

2009

View full text Add to dashboard Cite

show abstract

“…Liquiritigenin has various beneficial properties such as hepatoprotective [1], anti-cancer [6,7] and anti-inflammatory activity [4]. SIRT1 is a NAD + -dependent protein deacetylase that is involved in the regulation of gene expression, cell metabolism, and differentiation in many organisms including yeast, bacteria, and humans [36].…”

Section: Activation Of Sirt1 Expressionmentioning

confidence: 99%

“…It was found that liquiritin exist mainly as glucoside form of bioactive compound [4,5], while liquiritigenin, an aglycone of liquiritin is present in small amounts in G. uralensis [1]. There are several studies on their beneficial effects including prevention of carcinogenesis [6,7], hepatoprotective effects [1], and anti-inflammatory effects [4].…”

Section: Introductionmentioning

confidence: 99%

Production of Liquiritigenin with Cell-based Biotransformation and Its Anti-Aging Activity

Hwang¹,

Jeong²,

Park

2015

KSBB Journal

View full text Add to dashboard Cite

In this study, an efficient whole cell-based biotransformation for the production of liquiritigenin was developed using Laetiporus sulphureus CS0218 as biocatalyst and aqueous extracts of Glycyrrhiza uralensis as co-substrate, respectively. In order to determine the efficacy of this method, the optimal bioconversion conditions including mycelial growth, three important enzyme activities (β-glucosidase, α-rhamnosidase and β-xylosidase), and apparent viscosity of culture broth were monitored. After optimization, aqueous extracts of G. uralensis were added to the culture medium to directly produce algycone liquiritigenin. By applying this strategy, 67.5% of liquiritin was converted to liquiritigenin at pH 3.0 after 9 days of incubation and finally liquiritigenin was purified from the reaction mixture. And then, their biological activities including anti-oxidant and superoxide dismutase were observed. In fact, purified liquiritigenin was capable of bi-directional functions (i.e., either up-regulation or down-regulation of SIRT1 which is associated with aging). The results indicate that this strategy would be beneficial to produce biologically active liquiritigenin and could be used in pharmaceutical, cosmetic and food applications.

show abstract

“…Glycyrrhizin (GL), a major and active constituent of licorice root, has many attributed pharmacological effects, including anti-inflammatory [8] , anti-allergy [9] , and anti-viral activities [10] , which may be due to the steroid-like structure of 18β-glycyrrhetinic acid. Therefore, we hypothesized that GL could interact with β 2 -AR agonists at both the molecular and receptor level in a manner similar to glucocorticoids.…”

Section: Introductionmentioning

confidence: 99%

The synergistic anti-asthmatic effects of glycyrrhizin and salbutamol

et al. 2010

View full text Add to dashboard Cite

Aim: To investigate the efficacy of glycyrrhizin (GL) combined with salbutamol (SA) as an anti-asthma therapy. Methods: Rat lung beta2-adrenergic receptor (β 2 -AR) mRNA level was measured by real-time RT PCR. Intracellular cAMP accumulation was evaluated with a reporter gene assay. An in vitro acetylcholine-induced guinea pig tracheal strip contraction model was used to test the relaxing effects of GL and SA. The anti-inflammatory effects of GL and SA were tested using tumor necrosis factor-α-induced NF-κB transcriptional activation reporter assay, I-κB Western blotting and interleukin-8 ELISA. An in vivo guinea pig asthma model was used to prove further the synergistic effect of GL and SA. Results: GL (0.3 µmol/L) increased mRNA levels of β 2 -AR in vivo and the accumulation of cAMP in vitro. The combination of GL and SA also resulted in significant complementary anti-inflammatory effects via inhibition of NF-κB activation, degradation of I-κB and production of interleukin-8. A significant synergistic effect of the combination was detected both in vitro and in vivo in a guinea pig mode. Conclusion:The results demonstrate that GL and SA have synergistic anti-asthmatic effects and offer the possibility of a therapeutic application of GL in combination with β 2 -AR agonists in the treatment of asthma.

show abstract

Anti‐inflammatory effects of liquiritigenin as a consequence of the inhibition of NF‐κB‐dependent iNOS and proinflammatory cytokines production

Cited by 224 publications

References 46 publications

Liquiritigenin inhibits Aβ25–35-induced neurotoxicity and secretion of Aβ1–40 in rat hippocampal neurons

Liquiritigenin inhibits Aβ25–35-induced neurotoxicity and secretion of Aβ1–40 in rat hippocampal neurons

Production of Liquiritigenin with Cell-based Biotransformation and Its Anti-Aging Activity

The synergistic anti-asthmatic effects of glycyrrhizin and salbutamol

Contact Info

Product

Resources

About