Anti-Inflammatory Effects of Methanol Extract, Hexane, Ethyl Acetate, and Butanol Fraction of Piper crocatum Ruiz &amp; Pav Leaves on Lipopolysaccharide-induced RAW 264.7 Cells

Lely, Nilda; Arifin, Helmi; Aldi, Yufri; Wahyuni, Fatma Sri

doi:10.5530/pj.2021.13.169

Cited by 4 publications

(3 citation statements)

References 6 publications

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“…Ethno-botany and ethno-pharmacology of P. crocatum P. crocatum, used as spices, vegetables, components in some ethnic's ceremonial, and medicinal herbs by local community in Southeast Asian Countries especially Indonesia to treat candidiasis, hypertension, hepatitis, diabetes mellitus, kidney failure, cholesterol, preventing stroke, breast cancer, haemorrhoids, inflammation, coughing up blood, nose bleed, vaginal discharge, and tuberculosis disease (Astuti et al 2014;Lely et al 2021). P. crocatum, a plant native from Peru which then spread to several regions in the world, and grows in temperate climates (tropical and subtropical) (Lima et al 2020).…”

Section: Resultsmentioning

confidence: 99%

Phytochemical profiling of Piper crocatum and its antifungal mechanism action as Lanosterol 14 alpha demethylase CYP51 inhibitor: a review

et al. 2023

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Mycoses or fungal infections are general health problem that often occurs in healthy and immunocompromised people in the community. The development of resistant strains in Fungi and the incidence of azole antibiotic resistance in the Asia Pacific which reached 83% become a critical problem nowadays. To control fungal infections, substances and extracts isolated from natural resources, especially in the form of plants as the main sources of drug molecules today, are needed. Especially from Piperaceae, which have long been used in India, China, and Korea to treat human ailments in traditional medicine. The purpose of this review is to describe the antifungal mechanism action from Piper crocatum and its phytochemical profiling against lanosterol 14a demethylase CYP51. The methods used to search databases from Google Scholar to find the appropriate databases using Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA) Flow Diagram as a clinical information retrieval method. From 1.150.000 results searched by database, there is 73 final results article to review. The review shows that P. crocatum contains flavonoids, tannins, terpenes, saponins, polyphenols, eugenol, alkaloids, quinones, chavibetol acetate, glycosides, triterpenoids or steroids, hydroxychavikol, phenolics, glucosides, isoprenoids, and non-protein amino acids. Its antifungal mechanisms in fungal cells occur due to ergosterol especially lanosterol 14a demethylase (CYP51) inhibition, which is one of the main target sites for antifungal activity because it functions to maintain the integrity and function of cell membranes in Candida. P. crocatum has an antifungal activity through its phytochemical profiling against fungal by inhibiting the lanosterol 14a demethylase, make damaging cell membranes, fungal growth inhibition, and fungal cell lysis.

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Section: Resultsmentioning

confidence: 99%

Phytochemical profiling of Piper crocatum and its antifungal mechanism action as Lanosterol 14 alpha demethylase CYP51 inhibitor: a review

et al. 2023

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“…Based on the ethnobotany and ethnopharmacology data, the edible herbal plant P. crocatum was selected as a source to isolate new antifungal agents against C. albicans [35]. In the previous report, the extracts and active compounds of this plant were active with the following properties: antibacterial [36], antimicrobial [37], anticancer, anti-oxidant [38], antibiotics [30], antihyperglycemic [31], anti-inflammation [39], analgesic, immunomodulator, anti-tumor, and anti-fungal [40,41], respectively.…”

Section: Discussionmentioning

confidence: 99%

Antifungal Constituents of Piper crocatum and Their Activities as Ergosterol Biosynthesis Inhibitors Discovered via In Silico Study Using ADMET and Drug-Likeness Analysis

Siswina,

Rustama,

Sumiarsa

et al. 2023

Molecules

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Along with the increasing resistance of Candida spp. to some antibiotics, it is necessary to find new antifungal drugs, one of which is from the medicinal plant Red Betel (Piper crocatum). The purpose of this research is to isolate antifungal constituents from P. crocatum and evaluate their activities as ergosterol biosynthesis inhibitors via an in silico study of ADMET and drug-likeness analysis. Two new active compounds 1 and 2 and a known compound 3 were isolated, and their structures were determined using spectroscopic methods, while their bioactivities were evaluated via in vitro and in silico studies, respectively. Antifungal compound 3 was the most active compared to 1 and 2 with zone inhibition values of 14.5, 11.9, and 13.0 mm, respectively, at a concentration of 10% w/v, together with MIC/MFC at 0.31/1.2% w/v. Further in silico study demonstrated that compound 3 had a stronger ΔG than the positive control and compounds 1 and 2 with −11.14, −12.78, −12.00, and −6.89 Kcal/mol against ERG1, ERG2, ERG11, and ERG24, respectively, and also that 3 had the best Ki with 6.8 × 10−3, 4 × 10−4, 1.6 × 10−3, and 8.88 μM. On the other hand, an ADMET analysis of 1–3 met five parameters, while 1 had one violation of Ro5. Based on the research data, the promising antifungal constituents of P. crocatum allow P. crocatum to be proposed as a new antifungal candidate to treat and cure infections due to C. albicans.

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“…In previous studies, it has been reported that red betel leaf extract has been shown to have antiinflammatory effects (Wahjuni and Astawa 2016), (Ginting et al 2021), antidiabetic (Wahjuni et al 2017), antioxidants (Alfarabi et al 2010), antirheumatism (Maslikah et al 2019). From identifying phytochemical components, red betel leaf is reported to contain flavonoid, alkaloid, tannin-polyphenolic compounds, steroids, terpenoids and saponins (Lely et al 2021). Compounds that have been isolated from red betel leaf include pipercroside A and B phenolic glycosides (Li et al 2019);sitosterol and 2-(5', 6' dimethoxy-3', 4'-methylenedioxyphenyl)-6-(3", 4", 5"-trimethoxyphenyl)-3,7-dioxabicyclo [3,3,0] octane (Emrizal et al 2014), compound bicyclo[3.2.1]octanoid neolignans of guianin-type named as, (1′R, 2′R, 3′S, 7S, 8R)-Δ5′, 8′-2-acetoxy-3, 4, 5, 3′, 5′-pentamethoxy-4′oxo-8.1′,7.3′-neolignan (crocatin A) and (1′R, 2′R, 3′S, 7S, 8R)-Δ5′, 8′-2′-hydroxy-3, 4, 5, 3′, 5′-pentamethoxy-4′-oxo-8.1′,7.3′-neolignan (crocatin B) (Arbain et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Anti-Inflammatory Effect of Isolate Compounds from Ethyl Acetate Fraction of Piper crocatum Ruiz and Pav Leaves on Lipopolysaccharide-induced RAW 264.7 Cells

et al. 2022

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Red betel (Piper crocatum Ruiz and Pav) is a traditional Indonesian plant. The ethyl acetate fraction of red betel leaf has the potential as an anti-inflammatory. This research aimed to determine the anti-inflammatory effect of isolate compound from ethyl acetate fraction of Piper crocatum leaves on the production of cytokine pro-inflammatory TNF-α and ICAM-1 in lipopolysaccharide-induced RAW 264.7 cells. Cell viability was determined by the MTT method in the concentration range of 1.25; 2.5; 5; 10; 20, and 40 µg/ml. The TNF-α and ICAM-1 level was determined by the TNF-α and ICAM-1 assay kit as measured by an ELISA plate reader. Cell viability test of isolate compound from ethyl acetate fraction, the concentrations that gave viability percentages above 80% were 10; 5; 2.5 and 1.25 ug/ml. Isolate compound at concentrations of 10, 5, 2.5 and 1.25 ug/ml significantly inhibited TNF-α and ICAM-1 production with p<0.05 (p = 0.000).

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Anti-Inflammatory Effects of Methanol Extract, Hexane, Ethyl Acetate, and Butanol Fraction of Piper crocatum Ruiz & Pav Leaves on Lipopolysaccharide-induced RAW 264.7 Cells

Cited by 4 publications

References 6 publications

Phytochemical profiling of Piper crocatum and its antifungal mechanism action as Lanosterol 14 alpha demethylase CYP51 inhibitor: a review

Phytochemical profiling of Piper crocatum and its antifungal mechanism action as Lanosterol 14 alpha demethylase CYP51 inhibitor: a review

Antifungal Constituents of Piper crocatum and Their Activities as Ergosterol Biosynthesis Inhibitors Discovered via In Silico Study Using ADMET and Drug-Likeness Analysis

Anti-Inflammatory Effect of Isolate Compounds from Ethyl Acetate Fraction of Piper crocatum Ruiz and Pav Leaves on Lipopolysaccharide-induced RAW 264.7 Cells

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