AbstrakMinyak atsiri merupakan salah satu senyawa kimia dalam tanaman yang terbukti berpotensi sebagai antijamur. Beberapa penelitian menunjukkan bahwa minyak atsiri sereh wangi (Cymbopogon nardus L. Rendle) mempunyai aktivitas terhadap jamur patogen Tricophyton rubrum, Microsporum canis, dan Epidermophyton floccosum yang dapat menyebabkan penyakit dermatofitosis. Antijamur yang digunakan untuk pengobatan dermatofitosis ini, salah satunya adalah ketokonazol yang merupakan antijamur golongan imidazol. Pada penelitian ini ditentukan efektivitas antijamur kombinasi ketokonazol dengan minyak atsiri sereh wangi terhadap jamur penyebab dermatofitosis. Ekstraksi minyak atsiri menggunakan metode destilasi uap air. Uji efektivitas antijamur kombinasi ketokonazol dengan minyak atsiriri sereh wangi dengan metode difusi agar. Hasil penelitian kombinasi ketokonazol dengan minyak atsiri menunjukkan efek sinergis, karena diameter zona hambat ketokonazol mengalami peningkatan setelah dikombinasikan dengan minyak atsiri. Diameter zona hambat ketokonazol tunggal Tricophyton rubrum ATCC 28188 13,26 mm setelah dikombinasikan menjadi 24,9 mm, Microsporum canis ATCC 32699 12,8 mm setelah dikombinasikan menjadi 22,56 mm, Epidermophyton floccosum ATCC 52066 12,76 mm setelah dikombinasikan menjadi 19,5 mm. Hal ini disebabkan karena mekanisme kerja yang sinergis dari minyak atsiri sereh wangi (Cymbopogon nardus L. Rendle) dan ketokonazol.Kata kunci: citronella, dermatofitosis, sinergisAbstractEssential oil is one of the chemical compounds in plants that have been proven potential as an antifungal agent. Some studies showed that the essential oil of citronella (Cymbopogon nardus L. Rendle) could resist fungal pathogens Tricophyton rubrum, Microsporum canis and Epidermophyton floccosum, which cause dermatophytosis. Antifungals used for the treatment of dermatophytosis is ketoconazole, which belongs to imidazole group. The purpose of this study was to determine the effectiveness of antifungal ketoconazole in combination with essential oils of citronella against dermatophytosis. Extraction of essential oils was carried out using steam distillation. The effectiveness of antifungal ketoconazole in combination with citronella oil was measured using disk diffusion method. The result of the combination of ketoconazole with essential oils was synergistic as proven by the increased diameter of inhibition zone ketoconazole after combined with essential oils. Ketoconazole inhibition zone diameter single Tricophyton rubrum ATCC 28188 13.26 mm after combined into a 24.9 mm, Microsporum canis ATCC 32699 12.8 mm after combined into a 22.56 mm, Epidermophyton floccosum ATCC 52066 12.76 mm after combined into 19, 5 mm. This is because the synergitic mechanism of action of essential oils of citronella (Cymbopogon nardus L. Rendle) and ketoconazole.Key words: citronella, dermatophytosis, synergistic
Citronella fragrance is one of the many plants in Indonesia. Citronella fragrance is widely used by Indonesian people as a spice dish and has traditionally been used for various treatments. Citronella fragrance contains essential oils that have the potential to have anti-fungal effects and have traditionally been used for the treatment of skin diseases. Isolation of citronella essential oil with the method of vapor distillation. The tested fungi are opportunistic fungi that often infect human skin. In testing the antifungal activity of the citronella essential oil (Cymbopogonnardus (L.) Rendle) against the fungal causing skin infection. The research was done by using agar diffusion method and using the density disc as the diffusion medium against A research of antifungal�s activity of essential oil of citronella (Cymbopogon nardus (L.) Rendle ) to fungus that cause disease in humans has been done. This research used agar diffusion method to the fungus test Tricophyton rubrum, Tricophyton mentagrophytes and Candida albicans. The concentration that used 1%, 0,5%, 0,25% and 0,1%. The results showed that in concentration 1% essential oil of citronella (Cymbopogon nardus ( L.)Rendle) has the highest activity with Candida albicans with an average of inhibition diameter was 19,4mm � 0,15. In 0,1% concentration of essential oil of citronella (Cymbopogon nardus (L.) Rendle) does not have antifungal�s activity to Tricophyton mentagrophytes that without inhibition diameter around the paper discs. Meanwhile, in concentration 0,1%, the essential oil of citronella (Cymbopogon nardus (L.) Rendle) still have antifungal�s activity to Tricophyton rubrum and Candida albicans with an average of inhibition diameter 7,4 mm � 0,35 dan 8,5 mm � 0,15. Keywords: Candida albicans, Sereh wangi (Cymbopogonnardus (L.), Tricophyton rubrum, Tricophyton mentagrophytes, Candida albicans
Telah dilakukan penelitian aktivitas antibakteri minyak atsiri rimpang lengkuas merahterhadap bakteri penyebab diare. Isolasi minyak atsiri menggunakan metode destilasi uap airdengan rendemen 0,229%. Komponen kimia minyak atsiri dianalisis dengan menggunakanKromatografi Gas Spektrometer Masa. Dari hasil analisis KG-MS terdeteksi 21 senyawa dalamminyak atsiri rimpang lengkuas merah dimana senyawa terbesarnya yaitu eucalyptol (46,73%),beta farnesene (11,53%), beta-pinen (9,04%), 4-allylphenyl acetat (7,86%), dan caryophyllene(3,85%) Pengujian aktivitas antibakteri minyak atsiri rimpang lengkuas merah menggunakanmetode difusi agar terhadap bakteri Bacillus cereus ATCC 11778, Escherichia coli ATCC25922, dan Shigella dysentri ATCC 13313 dengan 5 variasi konsentrasi yaitu 10%, 20%, 30%,40% dan 50%. Hasil pengukuran zona bening dari minyak atsiri rimpang lengkuas merahmenunjukkan aktivitas terbesar pada konsentrasi 50% terhadap Bacillus cereus dengan rata-ratadiameter hambat 19,1 ± 0,77.
Tanaman kelengkeng (Dimoncarpus longan Lour) memiliki potensi sebagai sumber antioksidan alami. Senyawa metabolit sekunder yang berperan sebagai antioksidan tersebut diantaranya adalah fenol dan flavonoid. Penelitian ini bertujuan untuk menentukan kandungan fenol dan flavonoid total yang terkandung di dalam ekstrak daun kelengkeng (Dimoncarpus longan Lour). Sampel yang digunakan adalah daun kelengkeng segar dan kering yang diekstrasi dengam metode maserasi menggunakan pelarut etanol. Penetapan kandungan fenol total dilakukan menggunakan pereaksi Folin-Ciocalteu dan pereaksi AlCl3 untuk penetapan kandungan flavonoid total yang diukur menggunakan Spektrofotometer UV-Vis. Hasil penelitian menunjukkan bahwa ekstrak etanol daun segar dan daun kering mengandung senyawa metabolit sekunder fenol dan flavonoid. Kadar total fenol dari masing-masing ekstrak daun segar dan kering adalah sebesar 78,21 µg/mg dan 107,51 µg/mg ekstrak. Sedangkan untuk kadar flavonoid total masing-masing adalah 21,23 µg/mg untuk sampel daun segar dan 33,64 µg/mg untuk sampel daun kering. Ekstrak daun kering memiliki kandungan senyawa fenol dan flavonoid yang lebih tinggi dibandingkan ekstrak daun segar.Kata kunci: Folin-Ciocalteu, Kelengkeng (Dimoncarpus longan Lour), Total Fenol, Total Flavonoid
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Copyright © 2025 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.