No abstract

This research was aimed to study the immunomodulatory effect of ethanol extract of pineapple (Ananas comosus L. Merr) peel on white male mice with carbon clearance method. Animals  were divided into 5 groups: Group 1 was given tween 80 1 % v/v;Ggroup 2 was given meniran extracts; Group 3, 4 and 5 were given  ethanol extracts of pineapple peel with a dose of 125 mg/kg BW, 250 mg/kg BW, and 500 mg/kg BW,respectively, for 6 days orally. On the 7th day the animals were injected with carbon suspension 0.2 ml/20g BW at the tail vein. Blood was taken at 5th and 15th minutes using capillary pipe from the animal’s orbital retro vein, then it was diluted with 4 ml of 0.1% b/v sodium carbonate and measured its absorbance at 675 nm using visible spectrophotometer. The ethanol extract of pineapple peel showed immunomodulatory effect where the best dose was 250 mg/kg BW.  Keywords                : biological response modifiers, immunomodulatory, pineapple peel

Telah dilakukan penelitian aktivitas antibakteri minyak atsiri rimpang lengkuas merahterhadap bakteri penyebab diare. Isolasi minyak atsiri menggunakan metode destilasi uap airdengan rendemen 0,229%. Komponen kimia minyak atsiri dianalisis dengan menggunakanKromatografi Gas Spektrometer Masa. Dari hasil analisis KG-MS terdeteksi 21 senyawa dalamminyak atsiri rimpang lengkuas merah dimana senyawa terbesarnya yaitu eucalyptol (46,73%),beta farnesene (11,53%), beta-pinen (9,04%), 4-allylphenyl acetat (7,86%), dan caryophyllene(3,85%) Pengujian aktivitas antibakteri minyak atsiri rimpang lengkuas merah menggunakanmetode difusi agar terhadap bakteri Bacillus cereus ATCC 11778, Escherichia coli ATCC25922, dan Shigella dysentri ATCC 13313 dengan 5 variasi konsentrasi yaitu 10%, 20%, 30%,40% dan 50%. Hasil pengukuran zona bening dari minyak atsiri rimpang lengkuas merahmenunjukkan aktivitas terbesar pada konsentrasi 50% terhadap Bacillus cereus dengan rata-ratadiameter hambat 19,1 ± 0,77.

Telah dilakukan uji aktivitas antibakteri dari ekstrak etanol daun seledri (Apium graviolens L.) dan madu hutan terhadap bakteri penyebab penyakit kulit yaitu Staphylococcus aureus ATCC 25923, Streptococcus pyogenes ATCC 19615, Propionibacterium acnes ATCC 11827, Pseudomonas aeruginosa ATCC 27853, Sreptococcus β hemoliticus ATCC 19615. Ekstraksi dilakukan dengan cara maserasi menggunakan pelarut etanol destilat. pengujian aktivitas antibakteri dilakukan dengan menggunakan metode difusi agar dengan konsentrasi zat uji yaitu 70%:30%, 60%:40% dan 50%:50% dengan menggunakan etanol destilat sebagai kontrol negatif dan kloramfenikol sebagai kontrol positif. Hasil penelitian menunjukkan daya hambat ekstrak pada konsentrasi 70%:30% pada masing-masing bakteri uji yaitu Staphylococcus aureus ATCC 25923  sebesar 17 mm, Streptococcus pyogenes ATCC 19615 sebesar 18,6 mm, Propionibacterium acnes ATCC 11827 sebesar 17,9 mm, Pseudomonas aeruginosa ATCC 27853 sebesar 18,6 mm, Sreptococcus β hemoliticus ATCC 19615 sebesar 16,15 mm. hasil penelitian menunjukkan bahwa ekstrak etanol daun seledri dan madu hutan memiliki aktivitas antibakteri terhadap bakteri infeksi kulit dalam kategori kuat

The observation on anti-inflammatory effects ofextract of durian rind(Durio zibethinus Murray) has been done. Extractof durian rind(Durio zibethinus Murray) dose were: 100 mg/kg, 200 mg/kg and 400 mg/kg. Tween 80 2% was used as negative control and diclofenac sodium 0.9 mg/200g was used as positive control. The parameter was the percentage of inflammation observed in the left foot of male albino rats induced by 1% suspension of carrageen. Measurements of parameter was done every 30 minutes for 360 minutes. The results showed that percentage of anti-inflammatory of diclofenac sodium 0.9 mg/200g, the extract in dose of 400 mg/kg, 200 mg/kg and 100 mg/kg were 64.9%, 61.86%, 52.98% and 48.58%respectively. It can be concluded that ethanolic extract of durian rind(Durio zibethinus Murray) has an anti-inflammatory effects. The optimal dose as an anti-inflammatory agentwas 400mg/kg BW