Anti-Inflammatory Effects of Onions: Inhibition of Chemotaxis of Human Polymorphonuclear Leukocytes by Thiosulfinates and Cepaenes

Dorsch, Walter; Schneider, Eric L.; Bayer, Th.; Breu, W.; Wagner, Hildebert

doi:10.1159/000235221

Cited by 49 publications

(32 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, it has been used to treat inflammatory cell influx (16,17). Results of these experiments have shown that 10 g/ml of garlic extract suppresses the chemokine IL-8, a known chemoattractant for inflammatory cells, which may explain its therapeutic effect.…”

Section: Discussionmentioning

confidence: 99%

Allium sativum (garlic) suppresses leukocyte inflammatory cytokine production in vitro: Potential therapeutic use in the treatment of inflammatory bowel disease

2002

View full text Add to dashboard Cite

BackgroundCytokines involved in inflammatory bowel disease (IBD) direct a predominantly cell‐mediated T‐ helper‐1 (Th1) immune response. The nonspecific anti‐inflammatory treatment being used in the management of patients with IBD has not changed much since the 1970s and new therapeutic agents are keenly sought. Several compounds isolated from Allium sativum (garlic) modulate leukocyte cell proliferation and cytokine production.MethodsTo investigate the possible therapeutic effects of garlic in the treatment of patients with IBD, whole blood and peripheral blood mononuclear cells (PBMCs) were stimulated in the presence of various concentrations of garlic extract and the effect on leukocyte cytokine production was determined in vitro using multiparameter flow cytometry.ResultsMonocyte interleukin (IL)‐12 production was inhibited significantly in the presence of low concentrations of garlic extract (≥0.1 μg/ml total protein). Monocyte IL‐10 production increased significantly and monocyte tumor necrosis factor‐alpha (TNF‐α), IL‐1α, IL‐6, IL‐8, T‐cell interferon‐gamma (IFN‐γ), IL‐2, and TNF‐α decreased significantly in the presence of ≥10 μg/ml garlic extract. Twenty to fifty percent of the immunomodulatory activity of garlic extract on cytokine production was acid labile. The inhibitory activity of methylprednisolone, a commonly used anti‐inflammatory in IBD, with garlic on leukocyte cytokine production was additive.ConclusionsBy inhibiting Th1 and inflammatory cytokines while upregulating IL‐10 production, treatment with garlic extract may help to resolve inflammation associated with IBD. An in vivo animal model study needs to be undertaken to determine the significance of these in vitro findings. Cytometry 48:209–215, 2002. © 2002 Wiley‐Liss, Inc.

show abstract

Section: Discussionmentioning

confidence: 99%

Allium sativum (garlic) suppresses leukocyte inflammatory cytokine production in vitro: Potential therapeutic use in the treatment of inflammatory bowel disease

2002

View full text Add to dashboard Cite

show abstract

“…Recent literature is rich in both in vivo and in vitro studies reporting the antithrombotic (Lee et al, 2013), hypolipidemic (Lee et al, 2012b;Srinivasan, 2013), antidiabetic (Jung et al, 2011), antiobesity (Yoshinari et al, 2012), antioxidant (Alpsoy et al, 2013;Lee et al, 2012a), anti-inflammatory (Dorsch et al, 1990), cancer chemopreventive (Wang et al, 2012), and antiparasitic (Aboelhadid et al, 2013;Klimpel et al, 2011) properties of onion bulb extracts. Moreover, accumulated epidemiological studies suggest that a diet rich in onions may also have favorable effects on the risk of acute myocardial infarction (Galeone et al, 2009), and benign prostatic hyperplasia (Galeone et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Antioxidant activity of phenolic extracts from different cultivars of Italian onion (Allium cepa) and relative human immune cell proliferative induction

Lisanti

Formica

Ianni

et al. 2015

Pharmaceutical Biology

View full text Add to dashboard Cite

“…In fact, the bulk of the plethora of biological activities reported for Allium crops such as garlic and onion have been ascribed to the organosulfur small molecule derivatives of precursor S-substituted Cys sulfoxides, most notably the thiosulfinates and complex compounds that are formed from them that appear further downstream, such as di-, tri-, and polysulfides, cepaenes, and the aforementioned zwiebelanes. These molecules are reported to exhibit cardiovascular effects, antimicrobial activity, and anticancer activity (Bayer et al, 1989;Dorsch et al, 1990;Block, 1992;Iranshahi et al, 2008;Block, 2010). We have shown that petivericin and other thiosulfinates produced from P. alliacea S-substituted Cys sulfoxide precursors also exhibit significant antibacterial and antifungal activity (Kim et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Discovery and Characterization of a Novel Lachrymatory Factor Synthase inPetiveria alliaceaand Its Influence on Alliinase-Mediated Formation of Biologically Active Organosulfur Compounds

Musah

Kubec

2009

Plant Physiology

View full text Add to dashboard Cite

A novel lachrymatory factor synthase (LFS) was isolated and purified from the roots of the Amazonian medicinal plant Petiveria alliacea. The enzyme is a heterotetrameric glycoprotein comprised of two a-subunits (68.8 kD each), one g-subunit (22.5 kD), and one d-subunit (11.9 kD). The two a-subunits are glycosylated and connected by a disulfide bridge. The LFS has an isoelectric point of 5.2. It catalyzes the formation of a sulfine lachrymator, (Z)-phenylmethanethial S-oxide, only in the presence of P. alliacea alliinase and its natural substrate, S-benzyl-L-cysteine sulfoxide (petiveriin). Depending on its concentration relative to that of P. alliacea alliinase, the LFS sequesters, to varying degrees, the sulfenic acid intermediate formed by alliinase-mediated breakdown of petiveriin. At LFS:alliinase of 5:1, LFS sequesters all of the sulfenic acid formed by alliinase action on petiveriin, and converts it entirely to (Z)-phenylmethanethial S-oxide. However, starting at LFS:alliinase of 5:2, the LFS is unable to sequester all of the sulfenic acid produced by the alliinase, with the result that sulfenic acid that escapes the action of the LFS condenses with loss of water to form S-benzyl phenylmethanethiosulfinate (petivericin). The results show that the LFS and alliinase function in tandem, with the alliinase furnishing the sulfenic acid substrate on which the LFS acts. The results also show that the LFS modulates the formation of biologically active thiosulfinates that are downstream of the alliinase in a manner dependent upon the relative concentrations of the LFS and the alliinase. These observations suggest that manipulation of LFS-to-alliinase ratios in plants displaying this system may provide a means by which to rationally modify organosulfur small molecule profiles to obtain desired flavor and/or odor signatures, or increase the presence of desirable biologically active small molecules.

show abstract

Anti-Inflammatory Effects of Onions: Inhibition of Chemotaxis of Human Polymorphonuclear Leukocytes by Thiosulfinates and Cepaenes

Cited by 49 publications

References 8 publications

Allium sativum (garlic) suppresses leukocyte inflammatory cytokine production in vitro: Potential therapeutic use in the treatment of inflammatory bowel disease

Allium sativum (garlic) suppresses leukocyte inflammatory cytokine production in vitro: Potential therapeutic use in the treatment of inflammatory bowel disease

Antioxidant activity of phenolic extracts from different cultivars of Italian onion (Allium cepa) and relative human immune cell proliferative induction

Discovery and Characterization of a Novel Lachrymatory Factor Synthase inPetiveria alliaceaand Its Influence on Alliinase-Mediated Formation of Biologically Active Organosulfur Compounds

Contact Info

Product

Resources

About