Anti-Inflammatory Effects of the Mixture of Sorbus commixta, Urtica dioica, Phyllostachys nigra, and Rhus semialata Gall Extracts on LPS-induced Inflammation in HaCaT Cells

Lee, Kyung-Eun; Nam, Jin-Ju; Kim, Seon-Mi; Kim, Han-Kon; Moon, Shinje; Youm, Jong-Kyung

doi:10.15230/scsk.2014.40.1.45

Cited by 3 publications

(1 citation statement)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…현재까지 마가목에 대한 연구는 주로 마가자에 집중되어 있으며, 마가자로부터 sorbic acid [17], chalcone glycoside [2], lupeol 및 lupenone [23]의 분리, 항산화 활성 [6,11,14,20], 피부 광노화 억제 효과 [1], pancreatic lipase 저해 [19], hyaluronidase 저해 [6] 및 angiotensin converting enzyme 저해 [5], 혈관이완 활성 [9,31], 지질대사 개선 [7] 및 동맥 아테롬 발생억 제 [26], 항염증 활성 [10,15,32], 알코올 손상에 의한 간보호 효과 [18], 연골손상 억제 활성 [21], 암세포 생육억제 활성 [16] 및 살초활성 [12] (Table 5). …”

unclassified

In-vitro Antithrombosis Activity of Different Parts of Sorbus commixta from Ulleung Island

김미선¹,

성화정²,

손호용

2016

Journal of Life Science

View full text Add to dashboard Cite

Sorbus commixta, a flowering plant in the Rosaceae family, is native to Japan and Ulleung Island, Korea. This plant is also called maga-mok or mai-mok in Korea because the bud of the stem has a similar shape to the teeth of a horse. In this study, hot water extracts from different parts of S. commixta, such as leaf, stem, and immature and mature fruits, were prepared, and their antithrombosis and antioxidant activities were evaluated. The extraction yield and pH of stem extracts were 3.99% and 5.5, respectively. The stem extracts contained 89.2 mg/g of total polyphenols and 28.3 mg/g of total flavonoids. The hot water extracts prepared from the leaf, stem, immature, and mature fruit of S. commixta exhibited no hemolytic activity against human red blood cells, up to a concentration of 0.5 mg/ml. In an anticoagulation assay, the stem extracts showed strong extension in thrombin, prothrombin, and activated partial thromboplastin times, whereas the other extracts had no anticoagulation activity. In a platelet aggregation inhibitory activity assay, all the extracts tested had no inhibitory activity against human platelets. With regard to antioxidation activity, the stem extracts showed stronger radical scavenging activity and reducing power activity than the other extracts. The calculated RC50s, the concentration required for 50% radical scavenging activity, for DPPH anions, ABTS cations, and nitrite of the crude stem extracts were 119.7, 53.3, and 117.5 μg/ml, respectively, whereas they were 13.7, 5.2, and 14.9 μg/ml for DPPH anions, ABTS cations, and nitrite, respectively, for vitamin C. The results suggest that the stem extracts of S. commixta have strong potential for use as a novel resource for antithrombosis agents.Key words : Anticoagulation, anti-platelet aggregation, Sorbus commixta, stem extracts, Ulleungdo *Corresponding author *Tel : +82-54-820-5491, Fax : +82-54-820-7804 *E-mail : hysohn@anu.ac.kr This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

show abstract

unclassified

In-vitro Antithrombosis Activity of Different Parts of Sorbus commixta from Ulleung Island

김미선¹,

성화정²,

손호용

2016

Journal of Life Science

View full text Add to dashboard Cite

show abstract

Analysis of the Apoptotic Mechanisms of Snake Venom Toxin on Inflammation-induced HaCaT Cell-line

Chun

Song

2017

The Acupuncture

View full text Add to dashboard Cite

Objectives : Inthisstudy,therolesofInterleukin(IL)-4andSignaltransducerandactivatorof transcription6(STAT6),whichhavebeenreportedtoplayaroleinthepathogenesisofinflammationandcancer,wereevaluatedinsnakevenomtoxin(SVT)-inducedapoptosis. Methods : InflammationwasinducedinhumanHaCaTkerationocytes,bylipopolysaccharide (LPS;1μg/mL)ortumornecrosisfactor-α (TNF-α),followedbytreatmentwithSVT(0,1,or 2μg/mL).CellviabilitywasassessedbyMTTassaysafter24h,andtheexpressionoflevels ofIL-4,STAT6,andtheapoptosis-relatedproteinsp53,Bax,andBcl-2wereevaluatedby westernblotting.Electromobilityshiftassays(EMSAs)wereperformedtoevaluatetheDNA bindingcapacityofSTAT6. Results : MTTassaysshowedthatinflammation-inducedgrowthofHaCaTcellsfollowingLPSor TNF-α stimulationwasinhibitedbySVT.Westernblotanalysisshowedthatp53andBax, whichpromoteapoptosis,wereincreased,whereasthatofBcl-2,ananti-apoptoticprotein, wasdecreasedinaconcentration-dependentmannerinLPS-orTNF-α-inducedHaCaTcells followingtreatmentwithSVT.Moreover,followingtreatmentofHaCaTcellswithLPS,IL-4concentrationswereincreased,andtreatmentwithSVTfurtherincreasedIL-4expressionina concentration-dependentmanner.WesternblottingandEMSAsshowedthatthephosphory-latedformofSTAT6wasincreasedinHaCaTcellsinthecontextofLPS-orTNF-α-induced inflammationinaconcentration-dependentmanner,concomitantwithanincreaseintheDNA bindingactivityofSTAT6.

show abstract

Initial Toxicity Screening Techniques of Skin Contact Materials Using Glutathione

Heo¹,

An²,

Yoon³

et al. 2019

Asian J Beauty Cosmetol

View full text Add to dashboard Cite

Purpose: We developed a glutathione-containing screening (GCS) system capable of confirming toxicity at the substance screening stage aimed at reducing the time and cost of the cosmetic raw material development stage. Methods: Based on genotoxicity testing, a GCS system, comprising a direct and indirect system, was developed to reduce the time and cost to identify toxicity during the screening of candidate cosmetic substances. Toxicity potential was confirmed by identifying changes in the content of total glutathione (GSH), which conjugates only toxic substances, and those in the glutathione disulfide (GSSG)/GSH ratio. Results: Heptanal, a positive control substance in skin irritation tests, showed a decrease in total GSH and was identified as a direct-acting toxicant. Eugenol, a positive control substance in skin sensitization tests, showed a decrease in total GSH and an altered GSSG/GSH ratio and was identified as an indirect-acting toxicant. Conclusion: The GCS system could identify toxicity characteristics of skin irritation and sensitization. However, more positive chemicals are required to induce skin irritation or sensitization to challenge a chemical's toxic classification such as irritation or sensitization according to the GCS system, direct and indirect systems.

show abstract

Anti-Inflammatory Effects of the Mixture of Sorbus commixta, Urtica dioica, Phyllostachys nigra, and Rhus semialata Gall Extracts on LPS-induced Inflammation in HaCaT Cells

Cited by 3 publications

References 21 publications

In-vitro Antithrombosis Activity of Different Parts of Sorbus commixta from Ulleung Island

In-vitro Antithrombosis Activity of Different Parts of Sorbus commixta from Ulleung Island

Analysis of the Apoptotic Mechanisms of Snake Venom Toxin on Inflammation-induced HaCaT Cell-line

Initial Toxicity Screening Techniques of Skin Contact Materials Using Glutathione

Contact Info

Product

Resources

About