Anti-inflammatory effects of triptolide by inhibiting the NF-κB signalling pathway in LPS-induced acute lung injury in a murine model

Abstract

Abstract.Triptolide is one of the main active components in the Chinese herb Tripterygium wilfordii Hook F, which has been demonstrated to possess anti-inflammatory properties. The aim of this study was to investigate the effects of triptolide on lipopolysaccharide (LPS)-induced acute lung injury (ALI) in mice and to explore the possible mechanisms. Mice were administered LPS intranasally to induce lung injury, and triptolide was administered intraperitoneally 1 h prior to the LPS challenge. Triptolide-treated mice exhibited significantly reduced levels of leukocytes, myeloperoxidase activity and edema of the lung, as well as tumour necrosis factor-α, interleukin (IL)-1β and IL-6 production in the bronchoalveolar lavage fluid compared with LPS-treated mice. Additionally, western blot analysis showed that triptolide inhibited the LPS-induced phosphorylation of nuclear factor of κ light polypeptide gene enhancer in B cells inhibitor-α and nuclear factor κ-light-chain-enhancer of activated B cells-p65 (NF-κB p65) and the expression of Toll-like receptor 4 (TLR4). In conclusion, the results from the present study suggest that the anti-inflammatory effect of triptolide against LPS-induced ALI may be due to its ability to inhibit the TLR4-mediated NF-κB signalling pathway. Triptolide may therefore be a promising potential therapeutic agent for ALI treatment, which may ultimately aid the clinical therapy for patients with ALI.

Natural products triptolide, celastrol, and withaferin A inhibit the chaperone activity of peroxiredoxin I

“…Taken together, we demonstrate that natural products can serve as powerful chemical probes to precisely modulate their target proteins, and thus possess great potential to facilitate the biological study of multifunctional proteins. Considering that triptolide, withaferin A, and celastrol share anti-inflammatory,35–37 pro-apoptotic,38–40 and anti-angiogenic activities in common, further dissection of the correlation between their alkylation of Prx I and common bioactivities at the cellular level is an important next step and will be reported in due course.…”

MiR‐27 alleviates myocardial cell damage induced by hypoxia/reoxygenation via targeting TGFBR1 and inhibiting NF‐κB pathway

“…NF-κB refers to a number of DNA binding proteins that specifically bind to the κB site of the promoters of various genes and promotes transcription, which plays an important role in cell growth regulation, inflammatory responses, and immune responses. 28 Recently, there has been renewed interest in baicalin suppressed myocardial inflammation and apoptosis via inhibiting NF-κB pathway. 29 Huang et al hold the view that Arginase-2 protected rats myocardial I/R injury by suppressing inflammatory response through inhibition of NF-κB signaling.…”

Anti-inflammatory activity of a sulfated polysaccharide from the brown alga Sargassum cristaefolium

“…Upon activation, IkB is phosphorylated and released from the complex, and the Rel proteins migrate to the nucleus and bind specific consensus DNA elements on the target gene promoter to initiate production of various mediators (Karin & Ben-Neriah, 2000). Several anti-inflammatory drugs inhibit the production of proinflammatory cytokines by suppressing NF-kB activation (Shawish et al, 2014;Wang et al, 2014;Yang, Ham, & Choi, 2013). Thus, we used a commercial transcription factor ELISA kit to evaluate whether SCP modulated activation of NF-kB in LPS-stimulated RAW264.7 cells.…”