Anti-inflammatory effects of Zea mays L. husk extracts

Roh, Kyung‐Baeg; Kim, Hyo‐Young; Shin, Seung-woo; Kim, Youngsoo; Lee, Junga; Kim, Miok; Jung, Eunsun; Lee, Jongsung; Park, Deokhoon

doi:10.1186/s12906-016-1284-9

Cited by 22 publications

(12 citation statements)

References 28 publications

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“…Therefore, in the present study we investigated the in vitro effects of P. amarus by using LPS-activated U937 human macrophage, which mimics the inflammatory model [ 1 ]. Macrophages can be activated by LPS to produce pro-inflammatory molecules such as IL-6, TNF-α, IL-1β, PGE 2 and NO by triggering intracellular signaling pathways including NF-κB and MAPKs [ 1 , 2 , 35 ].…”

Section: Discussionmentioning

confidence: 99%

Anti-inflammatory effects of Phyllanthus amarus Schum. & Thonn. through inhibition of NF-κB, MAPK, and PI3K-Akt signaling pathways in LPS-induced human macrophages

Harikrishnan

Jantan

Haque

et al. 2018

BMC Complement Altern Med

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BackgroundPhyllanthus amarus has been used widely in various traditional medicines to treat swelling, sores, jaundice, inflammatory diseases, kidney disorders, diabetes and viral hepatitis, while its pharmacological and biochemical mechanisms underlying its anti-inflammatory properties have not been well investigated. The present study was carried out to investigate the effects of 80% ethanolic extract of P. amarus on pro-inflammatory mediators release in nuclear factor-kappa B (NF-кB), mitogen activated protein kinase (MAPK) and phosphatidylinositol 3-kinase/Akt (PI3K-Akt) signaling activation in lipopolysaccharide (LPS)-induced U937 human macrophages.MethodsThe release of prostaglandin E2 (PGE2) and pro-inflammatory cytokines, tumor necrosis factor (TNF)-α and interleukin (IL)-1β in a culture supernatant was determined by ELISA. Determination of cyclooxygenase-2 (COX-2) protein and the activation of MAPKs molecules (JNK, ERK and p38 MAPK), NF-κB and Akt in LPS-induced U937 human macrophages were investigated by immunoblot technique. The relative gene expression levels of COX-2 and pro-inflammatory cytokines were measured by using qRT-PCR. The major metabolites of P. amarus were qualitatively and quantitatively analyzed in the extract by using validated reversed-phase high performance liquid chromatography (HPLC) methods.ResultsP. amarus extract significantly inhibited the production of pro-inflammatory mediators (TNF-α, IL-1β, PGE2) and COX-2 protein expression in LPS-induced U937 human macrophages. P. amarus-pretreatment also significantly downregulated the increased mRNA transcription of pro-inflammatory markers (TNF-α, IL-1β, and COX-2) in respective LPS-induced U937 macrophages. It downregulated the phosphorylation of NF-κB (p65), IκBα, and IKKα/β and restored the degradation of IκBα, and attenuated the expression of Akt, JNK, ERK, and p38 MAPKs phosphorylation in a dose-dependent manner. P. amarus extract also downregulated the expression of upstream signaling molecules, TLR4 and MyD88, which play major role in activation of NF-κB, MAPK and PI3K-Akt signaling pathways. The quantitative amounts of lignans, phyllanthin, hypophyllahtin and niranthin, and polyphenols, gallic acid, geraniin, corilagin, and ellagic acid in the extract were determined by HPLC analysis.ConclusionThe study revealed that P. amarus targeted the NF-κB, MAPK and PI3K-Akt signaling pathways to exert its anti- inflammatory effects by downregulating the prospective inflammatory signaling mediators.Electronic supplementary materialThe online version of this article (10.1186/s12906-018-2289-3) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Anti-inflammatory effects of Phyllanthus amarus Schum. & Thonn. through inhibition of NF-κB, MAPK, and PI3K-Akt signaling pathways in LPS-induced human macrophages

Harikrishnan

Jantan

Haque

et al. 2018

BMC Complement Altern Med

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“…Water extract of PV (10% w/v) was prepared by overnight extraction in sterile water at 40 ~ 50°C under the condition of slow shaking. The extracts were filtered through filter paper and then frozen on a freezing tray for 48 h. A perfectly dried extract of PV was obtained after freeze drying of the liquid extract for 60 h. The obtained extract was dissolved in distilled water for further experiments [ 23 ].…”

Section: Methodsmentioning

confidence: 99%

Inhibitory Effects of Prunella vulgaris L. Extract on 11β‐HSD1 in Human Skin Cells

Roh

Park

Jung

2018

Evidence-Based Complementary and Alternative Medicine

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Glucocorticoids are a risk factor for age-induced skin structure and function defects, and the glucocorticoid-activating enzyme, 11β-hydroxysteroid dehydrogenase 1 (11β-HSD1), represents a promising therapeutic target. Prunella vulgaris L. (PV) is a perennial and an edible herbaceous plant normally cultivated in Asia and Europe. A recent study demonstrated a broad range of biological activities of PV including immune modulatory, antiviral, antiallergic, anti-inflammatory, antioxidant, and antidiabetic. However, little is known about the inhibitory effect of PV on 11β-HSD1. In this study, we investigated the inhibitory effect of Prunella vulgaris L. extract (PVE) and the underlying mechanism of 11β-HSD11 inhibition. Consistent with these results, cortisol levels were also reduced by PVE in vitro. The cortisone-induced translocation of glucocorticoids receptor (GR) was also attenuated. In addition, PVE inhibited a cortisone-mediated decrease in collagen content in skin. Collectively, these results suggest the beneficial effects of PVE in maintaining skin integrity.

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“…This effect enhanced the signaling of transforming growth factor beta (TGF-β), as well as promoted the degradation of the extracellular matrix [Li et al, 2012a]. Similarly, Roh et al [2016] investigated the anti-infl ammatory effects of Zea mays L. husk extracts and found a downregulation of the inducible nitric oxide synthase (iNOS) gene expression mediated by NF-κB and AP-1 signaling in RAW256.7 cells.…”

Section: Other Biological Effectsmentioning

confidence: 99%

Pigmented Maize (Zea mays L.) Contains Anthocyanins with Potential Therapeutic Action Against Oxidative Stress - A Review

Magaña-Cerino¹,

Peniche-Pavía²,

Tiessen³

et al. 2019

Pol. J. Food Nutr. Sci.

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Different maize (Zea mays L.) varieties have been used for thousands of years as a healthy food source in Mesoamerica including pigmented maize. Maize ingestion could contribute to the reduction in the rate of non-communicable diseases and, in turn, to its function as an adjuvant in their management. These diseases are mainly associated with oxidative stress, which is characterized by a redox cell imbalance produced due to pro-oxidant molecules accumulation, inducing irreversible damages. Although the endogenous antioxidant defense system is effi cient, exogenous antioxidants are necessary to help to prevent this damage. Bioactive compounds, like anthocyanins, contained in dietary plants exert a major activity against oxidative stress. Could the maize anthocyanins play a curative, preventive or complementary role in the treatment of chronic diseases? Here, we describe the occurrence of anthocyanins from pigmented maize and their chemical structures. Furthermore, the biosynthesis, bioavailability, and stability are also summarized. Finally, many in vitro and in vivo studies of maize anthocyanins are discussed that demonstrated their nutraceutical potential, antioxidant capacity, and other biological effects. Given the importance of the biological properties of maize anthocyanins, it is necessary to understand the current knowledge and propose further research or clinical studies which allows us to better elucidate the biological mechanism of maize anthocyanins derivatives of several varieties and processes of cooking and combination with other ingredients to enhance their nutritional and health benefi ts.

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Anti-inflammatory effects of Zea mays L. husk extracts

Cited by 22 publications

References 28 publications

Anti-inflammatory effects of Phyllanthus amarus Schum. & Thonn. through inhibition of NF-κB, MAPK, and PI3K-Akt signaling pathways in LPS-induced human macrophages

Anti-inflammatory effects of Phyllanthus amarus Schum. & Thonn. through inhibition of NF-κB, MAPK, and PI3K-Akt signaling pathways in LPS-induced human macrophages

Inhibitory Effects of Prunella vulgaris L. Extract on 11β‐HSD1 in Human Skin Cells

Pigmented Maize (Zea mays L.) Contains Anthocyanins with Potential Therapeutic Action Against Oxidative Stress - A Review

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