Anti-inflammatory properties of a wound dressing combination of zinc oxide and turmeric extract

Abstract

Aim:The aim of this study was to determine the effect of a wound dressing consisting of a zinc oxide with turmeric extract combination as an anti-inflammatory on the healing process through the expression of MAC387 and cyclooxygenase-2 (COX-2).Materials and Methods:Forty Wistar rats were divided into four control and four treatment groups (n=5). On day 1, a 6 mm×6 mm square of skin in the area of the vertebralis thoracis was excised from all subjects. The wound was then dressed with a combination of zinc oxide and turmeric extract for the treatment groups, while the control groups were left undressed. Both the control and treatment groups were then sequentially sacrificed on days 3, 5, 7, and 14 to obtain subepithelial excision samples. These samples subsequently underwent immunohistochemistry examination through the expression of MAC387 and COX-2 to ascertain the anti-inflammatory reaction to the wound healing process.Results:The highest expression of MAC387 was found in the treatment group to which a dressing of zinc oxide with turmeric extract had been applied on the day 5 before slowly reducing on days 7 and 14. MAC387 peaked in the undressed control group on day 14. The COX-2 expression results in control groups showed their higher expression on day 3, increased up to day 5, began to decline on day 7 before, and finally, decreasing on day 14. This result was different to those treatment groups which presented a high COX-2 expression on day 3, before gradually decreasing between days 5 and 7 and reaching its lowest point on day 14.Conclusion:A wound dressing consisting of a combination of zinc oxide and turmeric extract has been proven effective as an anti-inflammatory in the healing process.

Hyaluronic Acid (HA)‐Based Silk Fibroin/Zinc Oxide Core–Shell Electrospun Dressing for Burn Wound Management

“…The ZO as an anti‐inflammatory agent by decreasing the malondialdehyde level and blocking the pro‐inflammatory cytokines including interleukin‐1 (IL‐1), interleukin‐1Beta (IL‐1β), and tumor necrosis factor‐alpha (TNF‐α) decreases the inflammatory response in burned wound. [ 100,101 ] In addition, other reports further revealed that ZO mediated the toll‐like receptors/NF‐κB inflammatory signaling pathway, which is associated with decreased inflammation. [ 38 ]…”

<i>In silico</i> and <i>in vivo</i> anti-inflammatory studies of curcuminoids, turmeric extract with zinc oxide, and eugenol

“…This indicates the condition of control group on day 7 to still be in the inflammatory phase due to NF-κB releasing TNFα as a pro-inflammatory cytokine [2]. The A3 and B3 groups which were treated with a zinc oxide and eugenol dressing showed a lower expression of TNFα than the control group.…”

“…Subsequent immunohistochemistry evaluation adhered to the previously described method [2]. The procedural steps were completed with minor modifications [11].…”

“…Alternative dressings that could be applied to wounds include zinc oxide-turmeric rhizome extract that produces an anti-inflammatory effect [2]. Turmeric (Curcuma longa) is one herb proven to be safe because of its culinary uses and applications as a treatment for several diseases [3].…”

Neovascular pattern in wound healing after zinc oxide and Curcuma longa rhizome extract dressing application

“…Previous experiments have proven that the combination of zinc oxide wound dressing and the C. longa extract can hasten the wound healing process by decreasing the expression of toll-like receptors-2, nuclear factor κB (NF-κB), and tumor necrosis factor α in the inflammatory phase. [ 8 ] The nature of zinc oxide as antibacterial, disinfectant, and drying agent is the reason of zinc oxide use in medicine field. The previous experiments showed that the nature of zinc oxide can hasten the wound healing.…”