Anti‐invasion effects of 6‐shogaol and 6‐gingerol, two active components in ginger, on human hepatocarcinoma cells

Weng, Chia‐Jui; Wu, Cheng Feng; Huang, Hsiao Wen; Ho, Chi‐Tang; Yen, Gow‐Chin

doi:10.1002/mnfr.201000108

Cited by 124 publications

(86 citation statements)

References 35 publications

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“…This anti-H. pylori action was further confirmed by Mahady et al (2005) and Nostro et al (2006). Recently, Weng et al (2010) demonstrated that [6]-shogaol and [6]-gingerol exerted anti-invasive activity against human hepatocarcinoma cells.…”

Section: Zingiber Officinale Roscoe Zingiberaceae (Gengibre)mentioning

confidence: 62%

Bioactivity and potential therapeutic benefits of some medicinal plants from the Caatinga (semi-arid) vegetation of Northeast Brazil: a review of the literature

Silva¹,

Melo²,

Vasconcelos³

et al. 2012

Rev. bras. farmacogn.

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Abstract:Medicinal plants have been used in traditional medicine for several thousand years all over the world. In this sense, information from Brazilian ethnic groups on folk medicine have contributed to the discovery of pharmacological activities from various plant-derived agents potentially leading to the innovative drugs. The Caatinga (semi-arid) vegetation is a highly threatened biome, covering a vast area in northeastern Brazil and has suffered from strong human influence for many decades. Many plants species found in the Caatinga have been widely used in folk medicine and for commercial manufacturing of phytotherapeutic products. Thus, the present review aims to disseminate to the scientific community some known species of medicinal plants found in the Caatinga that have been studied and analyzed in pharmacological scientific assays. Among the species that stood out for their local importance and multiplicity of uses were: Amburana cearensisand Zingiber officinalis L. (gengibre). The present study shows that several herbal constituents from Caatinga plants, whose pharmacological actions have been well characterized, may be relevant candidates for future and innovative therapeutic development.

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Section: Zingiber Officinale Roscoe Zingiberaceae (Gengibre)mentioning

confidence: 62%

Bioactivity and potential therapeutic benefits of some medicinal plants from the Caatinga (semi-arid) vegetation of Northeast Brazil: a review of the literature

Silva¹,

Melo²,

Vasconcelos³

et al. 2012

Rev. bras. farmacogn.

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“…In vitro and in vivo findings have indicated that 6-shogaol exerts anti-inflammatory effects [12,13]. Recently, our laboratory and Weng et al have reported the in vitro anti-invasive effects of 6-shogaol against breast carcinoma and hepatocarcinoma cells respectively [14,15]. Shogaols have also been reported to possess antitumor effects and to date, findings have revealed that they are likely to induce cancer cell death via multiple mechanisms.…”

Section: Introductionmentioning

confidence: 91%

Shogaols at proapoptotic concentrations induce G2/M arrest and aberrant mitotic cell death associated with tubulin aggregation

et al. 2011

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Shogaols have been previously reported to induce cancer cell death via multiple mechanisms, among which one analog 6-shogaol has been reported to cause microtubule damage through specific reaction with sulfhydryl groups in tubulin. In this study, a series of shogaols with different side chain lengths (4-, 6-, 8- and 10-shogaol) was synthesized and evaluated for antiproliferative activity in HCT 116 colon carcinoma and SH-SY5Y neuroblastoma cells. 4- and 6-shogaol were identified as lead compounds possessing the strongest antiproliferative activity. In the soft agar assay, the lead shogaols displayed dose-dependent inhibition on cancer cell colony formation under anchorage-independent conditions. Using HCT 116 as the selected cancer cell line, the molecular events linking shogaols-induced G(2)/M cell cycle arrest to apoptosis characterized by caspase 3 and PARP cleavage were investigated. At sublethal concentrations, the halt at G(2)/M phase was alleviated along time and cells survived. Conversely, proapoptotic concentrations of 4- and 6-shogaol induced irreversible G(2)/M arrest that was at least in part associated with down-regulation of cell cycle checkpoint proteins cdk1, cyclin B and cdc25C, as well as spindle assembly checkpoint proteins mad2, cdc20 and survivin. A dose- and time-dependent accumulation of insoluble tubulin in the insoluble fractions of cell lysates provided evidence that G(2) checkpoint failure led to disruption of microtubule turnover. In summary, our results conclude that shogaols cause apoptosis by inducing aberrant mitosis at least through the attenuation of cell cycle and spindle assembly checkpoint proteins.

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“…The pungent vallinoids of ginger, [6]-gingerol and [6]-paradol, exhibit antiproliferation activity in liver, pancreatic, prostate, gastric, and leukemia cancer cells (Lee et al, 1998;Chen et al, 2007;Shukla and Singh, 2007). Furthermore, [6]-shogaol has also been shown to exhibit anticancer activities against breast cancer through the inhibition of cell invasion reduction of matrix metalloproteinase-9 expression (via blockade of nuclear factor activation) (Ling et al, 2010), anti-proliferation activity (through disruption of microtubule network of non-small lung epithelium cancer) (Choudhury et al, 2010), and antiinvasion on human hepatocellular cell (Weng et al 2010). To our knowledge, there has been no report on the anticancer activity of ginger against CCA.…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity, Toxicity, and Anticancer Activity of Zingiber Officinale Roscoe Against Cholangiocarcinoma

Plengsuriyakarn¹,

Viyanant²,

Eursitthichai³

et al. 2012

Asian Pacific Journal of Cancer Prevention

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Cholangiocarcinoma (CCA) is an uncommon adenocarcinoma which arises from the epithelial cells of the bile ducts. The aim of the study was to investigate the cytotoxicity, toxicity, and anticancer activity of a crude ethanolic extract of ginger (Zingiber officinale Roscoe) against CCA. Cytotoxic activity against a CCA cell line (CL-6) was assessed by calcein-AM and Hoechst 33342 assays and anti-oxidant activity was evaluated using the DPPH assay. Investigation of apoptotic activity was performed by DNA fragmentation assay and induction of genes that may be involved in the resistance of CCA to anticancer drugs (MDR1, MRP1, MRP2, and MRP3) was examined by real-time PCR. To investigate anti-CCA activity in vivo, a total of 80 OV and nitrosamine (OV/ DMN)-induced CCA hamsters were fed with the ginger extract at doses of 1000, 3000, and 5000 mg/kg body weight daily or every alternate day for 30 days. Control groups consisting of 10 hamsters for each group were fed with 5-fluorouracil (positive control) or distilled water (untreated control). Median IC 50 (concentration that inhibits cell growth by 50%) values for cytotoxicity and anti-oxidant activities of the crude ethanolic extract of ginger were 10.95, 53.15, and 27.86 μg/ml, respectively. More than ten DNA fragments were visualized and up to 7-9 fold up-regulation of MDR1 and MRP3 genes was observed following exposure to the ethanolic extract of ginger. Acute and subacute toxicity tests indicated absence of any significant toxicity at the maximum dose of 5,000 mg/kg body weight given by intragastric gavage. The survival time and survival rate of the CCA-bearing hamsters were significantly prolonged compared to the control group (median of 54 vs 17 weeks). Results from these in vitro and in vivo studies thus indicate promising anticancer activity of the crude ethanolic extract of ginger against CCA with the absence of any significant toxicity. Moreover, MDR1 and MRP3 may be involved in conferring resistance of CCA to the ginger extract.

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Anti‐invasion effects of 6‐shogaol and 6‐gingerol, two active components in ginger, on human hepatocarcinoma cells

Abstract: These results suggest that 6-shogaol and 6-gingerol might both exert anti-invasive activity against hepatoma cells through regulation of MMP-9 and TIMP-1 and that 6-shogaol could further regulate urokinase-type plasminogen activity.

Cited by 124 publications

References 35 publications

Bioactivity and potential therapeutic benefits of some medicinal plants from the Caatinga (semi-arid) vegetation of Northeast Brazil: a review of the literature

Bioactivity and potential therapeutic benefits of some medicinal plants from the Caatinga (semi-arid) vegetation of Northeast Brazil: a review of the literature

Shogaols at proapoptotic concentrations induce G2/M arrest and aberrant mitotic cell death associated with tubulin aggregation

Cytotoxicity, Toxicity, and Anticancer Activity of Zingiber Officinale Roscoe Against Cholangiocarcinoma

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