Anti-Itching and Anti-Inflammatory Effects of Kushenol F via the Inhibition of TSLP Production

Abstract

Atopic dermatitis (AD) is a chronic inflammatory skin disease that results from eczema, itching, disrupted barrier function and aberrant cutaneous immune responses. The aim of the present study was to assess the efficacy of kushenol F as an effective treatment for AD via the suppression of thymic stromal lymphopoietin (TSLP) production. The results of the present study demonstrated that the clinical symptoms of AD were less severe and there was reduced ear thickening and scratching behavior in kushenol F-treated Dermatophagoides farinae extract (DFE)/1-chloro-2,4-dinitrochlorobenzene (DNCB)-induced AD mice. Histopathological analysis demonstrated that kushenol F decreased the DFE/DNCB-induced infiltration of eosinophil and mast cells and TSLP protein expression levels. Furthermore, kushenol F-treated mice exhibited significantly lower concentrations of serum histamine, IgE and IgG2a compared with the DFE/DNCB-induced control mice. Kushenol F also significantly decreased phosphorylated NF-κB and IKK levels and the mRNA expression levels of IL-1β and IL-6 in cytokine combination-induced human keratinocytes. The results of the present study suggested that kushenol F may be a potential therapeutic candidate for the treatment of AD via reducing TSLP levels.

Liquid Chromatography/Tandem Mass Spectrometry Analysis of Sophora flavescens Aiton and Protective Effects against Alcohol-Induced Liver Injury and Oxidative Stress in Mice

“…SF has potential due to its various polyphenol and flavonoid constituents that have effects on suppressing ROS and NO production [71]. Among SF compounds, Epigallocatechin and Kurarinone have a significant antioxidant capacity [72,73], and Kushenol F and Isoxanthohumol decreased the phosphorylation of NF-κB and the levels of the proinflammatory cytokines [74,75]. It suggests that ESF is related to anti-inflammatory properties in alcoholic liver disease.…”

Therapeutic Application of Glutinous Rice Ferment Extract for Atopic Eczema

“…On the one hand, after TSLP activates DC, it can promote the proliferation and differentiation of naïve CD4 + T cells into inflammatory Th2 cells [4,5]. On the other hand, TSLP directly acts on natural immune cells, stimulating mast cells to produce a series of inflammatory factors to promote allergic reactions [6,7]. Simpson, a dermatologist at Oregon Health and Science University, believes that TSLP is the hub of contact between epithelial cells and the immune system and participated in a phase H trial of an anti-TSLP antibody called tezepe1umab to treat eczema [8].…”

Skin Care Function of Lactoferrin Was Characterized Using Recombinant Human Epidermal Model

“…The study by Ushasree et al [24] also confirmed that bLF at 2.5 mg/mL stimulated human limbal epithelial wound healing, and this stimulation was mediated by upregulation of platelet-derived growth factor (PDGF) or IL-6. The contents of IL-1a [25] and thymic stromal lymphopoietin protein (TSLP) [26] were used as corresponding indexes in a human skin anti-inflammatory test. IL-1 secretion is an important expression of KC cells in response to external stimuli and self-injury, and KC cells mainly contain IL-1α.…”