Anti-lipoxygenase, Radical Scavenging and Antimicrobial Activities of Lichen Species of Genus Heterodermia (Physciaceae)

Abstract

The aim of the research is to explore antilipoxygenase, radical scavenging and an ti mic ro bial activity of the ethyl acetate extract of the selected lichen spe cies of genus Heterodermia (Physciaceae . isidiophora showed the strong anti mic ro bial activity against most of the tested microorganisms at concentration ranging from 0.232 mg/ml to 0.591 mg/ml. These findings suggest that these li chen species of genus Heterodermia can be used as new bioresources for the na tu ral lipoxygenase inhibitor with antimicrobial and radical scavenging features. . Цель исследования -антилипоксигеназа, радикальная очистка и антимикробная активность этилацетатного экстракта видов лишайников ро да Heterodermia (Physciaceae). Экстрактам видов рода свойсвенно липоксигеназное инги бирование (LOX), зависящее от концентрации. Пятидесятипроцентное (IC 50 ) ингибирование LOX отмечалось у видов H. diademata, H. flabellata, H. an til la rum и H. incana при концентрациях 0,123 0,153, 0,160 и 0,150 мг/мл со от вет ст вен но; что меньше, чем у синтетических антиоксидантов BHA и BHT. Только экс тракт H. diademata показал IC 50 при концентрации 0,123 мг/мл равным LOX ин ги би тора индометацин. Кинетика LOX ингибирования экстрактов разных ви дов лишайников показывает разный режим ингибирования. Конкурентное ин ги бирование LOX отмечено у H. al bi cans и H. antillarum; неконкурентое -у H. dia de mata; отсутствие конкуренции характерно для H. angustiloba, H. fla bel la ta, H. in ca na, H. isidiophora и H. pseudospeciosa. Экстракты этих видов так же по ка за ли пятидесятипроцентную активность радикальной очистки. Этил аце тат ные экстракты H. diademata, H. angustiloba, H. albicans и H. isidiophora пока за ли силь ную антимикробную активность в отношении большинства про тес ти ро ван ных микроорганизмов в концентрациях от 0,232 до 0,591 мг/мл. Результаты по казывают, что виды лишайников Heterodermia могут быть использованы в ка честве нового биоресурса природной липоксигеназы с антимикробными и очистительными свойствами.

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“…No inhibitory activity against test bacteria was observed in case of DMSO. In an earlier study, Behera et al [9] showed the antibacterial effect of ethyl acetate extract of H. incana against Streptococcus faecalis with an minimum inhibitory concentration value of 1.624 mg/ml. Studies have shown the antibacterial potential of some species of Heterodermia such as Heterodermia diademata, [9,36] Heterodermia flabellata, [9] Heterodermia pseudospeciosa, [9] Heterodermia podocarpa, [39] Heterodermia Obscurata, [8,40] and Heterodermia boryi.…”

“…Lichens and their metabolites are shown to possess antibacterial activity against various pathogenic bacteria including antibiotic resistant bacteria. [9,[32][33][34][35][36][37][38] In this study, we evaluated the potential of H. incana to inhibit Gram-positive and Gram-negative bacteria by agar well diffusion method. The presence of an inhibition zone around the well is an indication of antibacterial potential of extract.…”

“…Apothecia are pedicellate with distinct margin (lecinulate) and about 8 mm in diameter. [19] The study of Behera et al [9] has shown the anti-lipoxygenase, antimicrobial and antioxidant potential of H. incana. In this study, we evaluated antibacterial and antifungal potential of an extract of H. incana.…”

“…Lichens are considered as one of the best indicators of air quality. [1][2][3][4][5][6][7][8][9] Since time immemorial, lichens have been used as sources of food, spice, medicine, and dye. Lichens have been considered to be a part of traditional medicine and are used to treat several human and veterinary ailments by various tribes of several countries.…”

“…Lichen extracts and the secondary metabolites of lichens are known to exhibit a range of bioactivities such as antimicrobial, antioxidant, insecticidal, anthelmintic, antiviral, anthelmintic, antiproliferative, anti-inflammatory, analgesic, and enzyme inhibitory activities. [3,6,7,9,[12][13][14][15][16] The lichen genus Heterodermia belongs to Physciaceae and is one among the most common lichens in tropical regions. The genus Heterodermia is distinguished from other foliose lichen genera of Physciaceae as the genus is characterized by the presence of the prosoplectenchymatous upper cortex in combination with atranorin as a cortical substance.…”