Anti-malarial and Anti-inflammatory Effects of Gynura procumbens are Mediated by Kaempferol via Inhibition of Glycogen Synthase Kinase-3β (GSK3β)

Sok, Kuan Wong; Lee, Michelle Sue Jann; Sudi, Suhaini; Hassan, Wan Rozianoor Mohd; Lee, Ping Chin; Embi, Noor; Sidek, Hasidah Mohd

doi:10.17576/jsm-2015-4410-15

Cited by 15 publications

(4 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As a flavonoid, kaempferol is able to inhibit oxidative stress, ROS formation, and lipid peroxidation [21–23], and there is a correlation between its antioxidant and antimalarial activities [4]. In addition, inhibition of glycogen synthase kinase-3 β (GSK3 β ) of the malaria parasite has been reported to be a molecular basis of the antimalarial effect of kaempferol [24]. GSK3 has been described to play a critical role in the host response to malaria infection [25].…”

Section: Discussionmentioning

confidence: 99%

Antimalarial Activity of Kaempferol and Its Combination with Chloroquine in Plasmodium berghei Infection in Mice

Somsak

Damkaew

Onrak

2018

Journal of Pathogens

View full text Add to dashboard Cite

The search for new antimalarial drugs has become an urgent requirement due to resistance to the available drugs and the lack of an effective vaccine. In this respect, the present study aimed to evaluate the antimalarial activity of kaempferol against Plasmodium berghei infection in mice as an in vivo model. Chronic toxicity and antimalarial activities of kaempferol alone and in combination with chloroquine were investigated in P. berghei ANKA infected ICR mice using standard procedures. The results showed that chronic administration of 2,000 mg/kg of kaempferol resulted in no overt signs of toxicity as well as no hepatotoxicity, nephrotoxicity, or hematotoxicity. Interestingly, kaempferol exerted significant (P < 0.05) chemosuppressive, chemoprophylactic, and curative activities in a dose-dependent manner. The highest antimalarial activity was found at a dose of 20 mg/kg which resulted in a significantly (P < 0.05) prolonged survival of infected mice. Moreover, combination treatment of chloroquine and kaempferol also presented significant (P < 0.05) antimalarial effects, although the effects were not significantly different from the chloroquine treated group. From the results of the present study, it can be concluded that kaempferol possesses acceptable antimalarial activities. However, further investigation should be undertaken on the mechanism responsible for the observed antimalarial activity.

show abstract

Section: Discussionmentioning

confidence: 99%

Antimalarial Activity of Kaempferol and Its Combination with Chloroquine in Plasmodium berghei Infection in Mice

Somsak

Damkaew

Onrak

2018

Journal of Pathogens

View full text Add to dashboard Cite

show abstract

“…On the contrary, G. procumbens belonging to the family Asteraceae is commonly known as longevity spinach that grows broadly in India, Bangladesh, Southeast Asia, especially in Indonesia, Malaysia, and Thailand. Some of the traditional healing claims have been verified in scientific and pharmacological studies, including anti-herpes virus [ 6 ], anti-inflammatory [ 7 , 8 ], anti-hyperlipidemic, anti-hyperglycemic [ 9 – 14 ], and anti-hypertensive activities [ 15 – 18 ]. Recently, particular attention has been given to G. procumbens in the pharmacology of antidiabetic medicinal plants perhaps because of its test data and efficiency in managing diabetes mellitus.…”

Section: Introductionmentioning

confidence: 99%

In Vivo and In Silico Assessment of Diabetes Ameliorating Potentiality and Safety Profile of Gynura procumbens Leaves

Tahsin

Tithi

Mim

et al. 2022

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Background. Diabetes mellitus is one of the most notable health dilemmas. Analyzing plants for new antidiabetic remedies has become an impressive territory for life science researchers. Gynura procumbens has long been used to treat diabetes. Thus, we strived to ascertain the hypoglycemic potentiality of extract of leaves of G. procumbens by in vivo and in silico approaches. Methods. Fresh leaves of G. procumbens were collected and shade-dried to prepare ethanolic extracts to evaluate pharmacological parameters. Diabetes was induced in rats via injecting alloxan through the intraperitoneal route at a dose of 150 mg/kg body weight. Humalyzer 3000 was used to perform a biochemical assay of collected samples from rats. Anti-hyperglycemic activity study along with overdose toxicity test was performed. The pharmacological activity of this plant was also evaluated through a molecular docking study. This in silico study investigated the binding affinity of natural ligands from G. procumbens against glycoside hydrolase enzymes. Results. We detected a peak plasma concentration of G. procumbens at 3 hours 45 minutes that is roughly similar to the peak plasma concentration of metformin. Again, in OGTT and anti-hyperglycemic tests, it has been ascertained that both plant extract and metformin can exert significant P < 0.05 and highly significant P < 0.01 hypoglycemic activity in a dose-dependent manner. Metformin exhibited better therapeutic efficacy than that of plant extract, but it possessed null statistical significance. Also, our safety profile expressed that, similar to metformin, the plant extract can restore the disturbed pathological state in a dose-oriented approach with a wide safety margin. In silico study also validated the potentialities of natural constituents of G. procumbens. Conclusion. This study suggested that G. procumbens can be considered as potential antidiabetic plant. Robust and meticulous investigation regarding plant chemistry and pharmacology in the future may bring about a new dimension that will aid in discovering antidiabetic drugs from this plant in the diabetes management system.

show abstract

“…However, Jansen et al [ 77 ], reported a lack of anti-plasmodial properties of kaempferol in vitro, although the researchers isolated the molecule from medicinal plants other than those of the Asteraceae family. Kaempferol also acts as an inhibitor of glycogen synthase kinase-3β (GSK3β) of the malaria parasite [ 84 ]; hence, it blocks erythrocyte invasion by the parasite. Barlianna et al [ 85 ] confirmed the moderate anti-plasmodial properties of a derivative of kaempferol (kaempferol-3- O -rhamnoside) in P. falciparum , with a reported IC 50 value of 106 μM.…”

Section: Resultsmentioning

confidence: 99%

Secondary Metabolites Isolated from Artemisia afra and Artemisia annua and Their Anti-Malarial, Anti-Inflammatory and Immunomodulating Properties—Pharmacokinetics and Pharmacodynamics: A Review

et al. 2023

View full text Add to dashboard Cite

There are over 500 species of the genus Artemisia in the Asteraceae family distributed over the globe, with varying potentials to treat different ailments. Following the isolation of artemisinin (a potent anti-malarial compound with a sesquiterpene backbone) from Artemisia annua, the phytochemical composition of this species has been of interest over recent decades. Additionally, the number of phytochemical investigations of other species, including those of Artemisia afra in a search for new molecules with pharmacological potentials, has increased in recent years. This has led to the isolation of several compounds from both species, including a majority of monoterpenes, sesquiterpenes, and polyphenols with varying pharmacological activities. This review aims to discuss the most important compounds present in both plant species with anti-malarial properties, anti-inflammatory potentials, and immunomodulating properties, with an emphasis on their pharmacokinetics and pharmacodynamics properties. Additionally, the toxicity of both plants and their anti-malaria properties, including those of other species in the genus Artemisia, is discussed. As such, data were collected via a thorough literature search in web databases, such as ResearchGate, ScienceDirect, Google scholar, PubMed, Phytochemical and Ethnobotanical databases, up to 2022. A distinction was made between compounds involved in a direct anti-plasmodial activity and those expressing anti-inflammatory and immunomodulating activities or anti-fever properties. For pharmacokinetics activities, a distinction was made between compounds influencing bioavailability (CYP effect or P-Glycoprotein effect) and those affecting the stability of pharmacodynamic active components.

show abstract

Anti-malarial and Anti-inflammatory Effects of Gynura procumbens are Mediated by Kaempferol via Inhibition of Glycogen Synthase Kinase-3β (GSK3β)

Abstract: Gynura procumbens is a medicinal plant, traditionally used to treat inflammation and fever. A yeast-based assay detected

Cited by 15 publications

References 52 publications

Antimalarial Activity of Kaempferol and Its Combination with Chloroquine in Plasmodium berghei Infection in Mice

Antimalarial Activity of Kaempferol and Its Combination with Chloroquine in Plasmodium berghei Infection in Mice

In Vivo and In Silico Assessment of Diabetes Ameliorating Potentiality and Safety Profile of Gynura procumbens Leaves

Secondary Metabolites Isolated from Artemisia afra and Artemisia annua and Their Anti-Malarial, Anti-Inflammatory and Immunomodulating Properties—Pharmacokinetics and Pharmacodynamics: A Review

Contact Info

Product

Resources

About