Anti-MRSA Properties of Prodigiosin from Serratia sp. PDGS 120915

Ji, Keunho; Jeong, Tae Hyug; Kim, Young Tae

doi:10.5352/jls.2015.25.1.29

Cited by 7 publications

(4 citation statements)

References 21 publications

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“…In this study, the MIC value of prodigiosin ranged from 2.5 to 5 mg/L for all S. aureus isolates. This was inconsistent with previously reported MIC values, which were in the range of 0.25–32 mg/L ( Ji et al, 2015 ; Danevčič et al, 2016b ; Suryawanshi et al, 2017 ; Wang B. et al, 2022 ; Wang Y. J. et al, 2022 ; Yan et al, 2023 ). Possible reasons for the wide range of MIC values reported in the literature may be prodigiosin samples obtained from different strains, different purification methods, as well as different methods for measuring MIC values and different testing strains.…”

Section: Discussioncontrasting

confidence: 99%

Transcriptomic analysis of cell envelope inhibition by prodigiosin in methicillin-resistant Staphylococcus aureus

Liu,

Wang,

You

et al. 2024

Front. Microbiol.

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Methicillin-resistant Staphylococcus aureus (MRSA) is a leading threat to public health as it is resistant to most currently available antibiotics. Prodigiosin is a secondary metabolite of microorganisms with broad-spectrum antibacterial activity. This study identified a significant antibacterial effect of prodigiosin against MRSA with a minimum inhibitory concentration as low as 2.5 mg/L. The results of scanning electron microscopy, crystal violet staining, and confocal laser scanning microscopy indicated that prodigiosin inhibited biofilm formation in S. aureus USA300, while also destroying the structure of the cell wall and cell membrane, which was confirmed by transmission electron microscopy. At a prodigiosin concentration of 1.25 mg/L, biofilm formation was inhibited by 76.24%, while 2.5 mg/L prodigiosin significantly reduced the vitality of MRSA cells in the biofilm. Furthermore, the transcriptomic results obtained at 1/8 MIC of prodigiosin indicated that 235and 387 genes of S. aureus USA300 were significantly up- and downregulated, respectively. The downregulated genes were related to two-component systems, including the transcriptional regulator LytS, quorum sensing histidine kinases SrrB, NreA and NreB, peptidoglycan biosynthesis enzymes (MurQ and GlmU), iron-sulfur cluster repair protein ScdA, microbial surface components recognizing adaptive matrix molecules, as well as the key arginine synthesis enzymes ArcC and ArgF. The upregulated genes were mainly related to cell wall biosynthesis, as well as two-component systems including vancomycin resistance-associated regulator, lipoteichoic acid biosynthesis related proteins DltD and DltB, as well as the 9 capsular polysaccharide biosynthesis proteins. This study elucidated the molecular mechanisms through which prodigiosin affects the cell envelope of MRSA from the perspectives of cell wall synthesis, cell membrane and biofilm formation, providing new potential targets for the development of antimicrobials for the treatment of MRSA.

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Section: Discussioncontrasting

confidence: 99%

Transcriptomic analysis of cell envelope inhibition by prodigiosin in methicillin-resistant Staphylococcus aureus

Liu,

Wang,

You

et al. 2024

Front. Microbiol.

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“…coli was 103.4 ± 6.3 μg/mL [ 25 ], purified prodigiosin from Serratia sp. PDGS 120915 has an MIC value of 32 μg/mL against MRSA [ 76 ] and purified prodigiosin from S . marcescens UFPEDA 398 had low MIC values (1.0–4.0 μg/mL) against eighteen oxacillin-resistant S .…”

Section: Discussionmentioning

confidence: 99%

“…For example, the MIC of prodigiosin purified from Vibrio ruber DSM14379 towards E. coli was 103.4 ± 6.3 μg/mL [25], purified prodigiosin from Serratia sp. PDGS 120915 has an MIC value of 32 μg/mL against MRSA [76] and purified prodigiosin from S. marcescens UFPEDA 398 had low MIC values (1.0-4.0 μg/mL) against eighteen oxacillin-resistant S. aureus (ORSA) clinical strains [58]. Although the absorption spectra, HPLC and LC-MS/MS analyses (Figs 2 and 3) showed that prodigiosin is a predominant compound in the crude extract, nevertheless, the higher MIC value may likely be a result of minor impurities within the crude prodigiosin extract which could have impeded the action of prodigiosin or its interaction with the bacterial membrane, thus, requiring higher prodigiosin concentrations to inhibit bacterial growth.…”

Section: Plos Onementioning

confidence: 99%

Prodigiosin inhibits bacterial growth and virulence factors as a potential physiological response to interspecies competition

et al. 2021

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Prodigiosin, a red linear tripyrrole pigment, has long been recognised for its antimicrobial property. However, the physiological contribution of prodigiosin to the survival of its producing hosts still remains undefined. Hence, the aim of this study was to investigate the biological role of prodigiosin from Serratia marcescens, particularly in microbial competition through its antimicrobial activity, towards the growth and secreted virulence factors of four clinical pathogenic bacteria (methicillin-resistant Staphylococcus aureus (MRSA), Enterococcus faecalis, Salmonella enterica serovar Typhimurium and Pseudomonas aeruginosa) as well as Staphylococcus aureus and Escherichia coli. Prodigiosin was first extracted from S. marcescens and its purity confirmed by absorption spectrum, high performance liquid chromatography (HPLC) and liquid chromatography-tandem mass spectrophotometry (LC-MS/MS). The extracted prodigiosin was antagonistic towards all the tested bacteria. A disc-diffusion assay showed that prodigiosin is more selective towards Gram-positive bacteria and inhibited the growth of MRSA, S. aureus and E. faecalis and Gram-negative E. coli. A minimum inhibitory concentration of 10 μg/μL of prodigiosin was required to inhibit the growth of S. aureus, E. coli and E. faecalis whereas > 10 μg/μL was required to inhibit MRSA growth. We further assessed the effect of prodigiosin towards bacterial virulence factors such as haemolysin and production of protease as well as on biofilm formation. Prodigiosin did not inhibit haemolysis activity of clinically associated bacteria but was able to reduce protease activity for MRSA, E. coli and E. faecalis as well as decrease E. faecalis, Salmonella Typhimurium and E. coli biofilm formation. Results of this study show that in addition to its role in inhibiting bacterial growth, prodigiosin also inhibits the bacterial virulence factor protease production and biofilm formation, two strategies employed by bacteria in response to microbial competition. As clinical pathogens were more resistant to prodigiosin, we propose that prodigiosin is physiologically important for S. marcescens to compete against other bacteria in its natural soil and surface water environments.

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“…The combination of natural pigments with traditional antibiotics may represent a strategy to counteract the spread of superbugs. In this context, PG in combination with β‐lactamic antibiotics showed a synergistic effect against a MRSA strain 90 .…”

Section: Biotechnological Applications Of Bacterial Pigmentsmentioning

confidence: 91%

Bacterial pigments: A colorful palette reservoir for biotechnological applications

Orlandi

Martegani

Giaroni

et al. 2021

Biotech and App Biochem

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Synthetic derivatives are currently used instead of pigments in many applicative fields, from food to feed, from pharmaceutical to diagnostic, from agronomy to industry. Progress in organic chemistry allowed to obtain rather cheap compounds covering the whole color spectrum. However, several concerns arise from this chemical approach, as it is mainly based on nonrenewable resources such as fossil oil, and the toxicity or carcinogenic properties of products and/or precursors may be harmful for personnel involved in the productive processes. In this scenario, microorganisms and their pigments represent a colorful world to discover and reconsider. Each living bacterial strain may be a source of secondary metabolites with peculiar functions. The aim of this review is to link the physiological role of bacterial pigments with their potential use in different biotechnological fields. This enormous potential supports the big challenge for the development of strategies useful to identify, produce, and purify the right pigment for the desired application. At the end of this ideal journey through the world of bacterial pigments, the attention will be focused on melanin compounds, whose production relies upon different techniques ranging from natural producers, heterologous hosts, or isolated enzymes. In a green workflow, the microorganisms represent the starting and final point of pigment production.

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Anti-MRSA Properties of Prodigiosin from Serratia sp. PDGS 120915

Cited by 7 publications

References 21 publications

Transcriptomic analysis of cell envelope inhibition by prodigiosin in methicillin-resistant Staphylococcus aureus

Transcriptomic analysis of cell envelope inhibition by prodigiosin in methicillin-resistant Staphylococcus aureus

Prodigiosin inhibits bacterial growth and virulence factors as a potential physiological response to interspecies competition

Bacterial pigments: A colorful palette reservoir for biotechnological applications

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