Anti-myeloperoxidase antibodies attenuate the monocyte response to LPS and shape macrophage development

Oxidized phospholipids are proinflammatory and proatherogenic in hypercholesterolaemic mice

“…For example, OxPLs can activate cells by a variety of receptors, including CD36, TLR2/1 and 6, TLR4, CD14 and combinations of these receptors, and in turn, generate a wide variety of responses 1,5,7,8,16,22-25 . In addition, the PC on OxLDL mediates macrophage uptake by CD36 and SR-B1 5 .…”

Neutrophils are not consistently activated by antineutrophil cytoplasmic antibodies in vitro

“…The results are not due to inactivity of the purified ANCA IgG preparations. Aliquots of the same ANCA and control IgG batches were used in a recent publication where we demonstrated clear effects of these ANCA IgG preparations on monocytes, in experiments performed with during the same period of time 310.1136/annrheumdis-2018-214405.supp1 …”

4C3 Human Monoclonal Antibody: A Proof of Concept for Non-pathogenic Proteinase 3 Anti-neutrophil Cytoplasmic Antibodies in Granulomatosis With Polyangiitis

“…but not from PR3-ANCA positive patients were used by the same group, in another study, and promoted inflammation through monocyte stimulation (60). Another study suggested that neutrophil activation induced by IgG ANCA, mostly observed in the literature, could be related to the persistence, after purification, of elements from patient sera and not to IgG ANCA (61).…”

“…Indeed, purified IgG from AAV patient sera, even in active disease, did not induce neutrophil activation from two different healthy donors ( 59 ). Interestingly, the same IgG preparations obtained from MPO-ANCA positive patients but not from PR3-ANCA positive patients were used by the same group, in another study, and promoted inflammation through monocyte stimulation ( 60 ). Another study suggested that neutrophil activation induced by IgG ANCA, mostly observed in the literature, could be related to the persistence, after purification, of elements from patient sera and not to IgG ANCA ( 61 ).…”