Anti-Obesity and Anti-Hyperglycemic Effects of Meretrix lusoria Protamex Hydrolysate in ob/ob Mice

Abstract

Meretrix lusoria (M. lusoria) is an economically important shellfish which is widely distributed in South Eastern Asia that contains bioactive peptides, proteins, and enzymes. In the present study, the extracted meat content of M. lusoria was enzymatic hydrolyzed using four different commercial proteases (neutrase, protamex, alcalase, and flavourzyme). Among the enzymatic hydrolysates, M. lusoria protamex hydrolysate (MLPH) fraction with MW ≤ 1 kDa exhibited the highest free radical scavenging ability. The MLPH fraction was further purified and an amino acid sequence (KDLEL, 617.35 Da) was identified by LC-MS/MS analysis. The purpose of this study was to investigate the anti-obesity and anti-hyperglycemic effects of MLPH containing antioxidant peptides using ob/ob mice. Treatment with MLPH for 6 weeks reduced body and organ weight and ameliorated the effects of hepatic steatosis and epididymal fat, including a constructive effect on hepatic and serum marker parameters. Moreover, hepatic antioxidant enzyme activities were upregulated and impaired glucose tolerance was improved in obese control mice. In addition, MLPH treatment markedly suppressed mRNA expression related to lipogenesis and hyperglycemia through activation of AMPK phosphorylation. These findings suggest that MLPH has anti-obesity and anti-hyperglycemic potential and could be effectively applied as a functional food ingredient or pharmaceutical.

New Insights into Antioxidant Peptides: An Overview of Efficient Screening, Evaluation Models, Molecular Mechanisms, and Applications

“…Meanwhile, MLPH significantly inhibited adipogenesis and hyperglycemia-related mRNA expression. These results demonstrate the anti-obesity and anti-hyperglycemia potential of MLPH [64]. Similarly, the streptozocin-induced type 1 diabetes mice were used to evaluate the activity of Harpadon nehereus bones collagen peptide (HNCP).…”

Bioactive compounds from Sargassum horneri attenuates inflammation and obesity regulating by Nrf2/HO-1 and AMPK signaling pathways

“…Antioxidant activities of S. horneri 70% EtOH extract and its subtractions S. horneri ethanolic extract and its subfractions antioxidant activity were evaluated by scavenging DPPH and ABTS radicals in vitro as described earlier (Kim et al 2022). In brief, methanol was used to dissolve 0.20mM of DPPH (2,2-diphenyl-1-picrylhydrazyl; #1898664, Sigma-Aldrich, St. Louis, MO, USA) solution.…”

“…Moreover, protein was measured by the biuret reaction (#B3934), albumin was measured with a colorimetric method (#MAK124). These levels were used to assessment toxicities to liver and kidney (Kim et al 2022).…”

Spirulina platensis peptide-loaded nanoliposomes alleviate hepatic lipid accumulation in Male Wistar Rats by influencing redox homeostasis and lipid metabolism via the AMPK signaling pathway

“…Bioactive peptides (BAPs) are short chains of amino acids derived from food proteins that have been shown to elicit bene cial physiological effects in humans (Nardo et al, 2020;Purohit et al, 2024;Suryaningtyas and Je, 2023). The identi cation of biological functions including the lowering of lipid levels and blood pressure, antithrombotic characteristics, as well as antioxidant and anti-in ammatory properties, offers a theoretical basis for further research on bioactive peptides(Chaves Filho et al, 2020; Kim et al, 2022;Suryaningtyas and Je, 2023).…”