Anti-oxidant and Anti-inflammatory Effects of Sinapic Acid in UVB Irradiation-Damaged HaCaT Keratinocytes

Lim, Kye Hwa; Ku, Jung-Eun; Rhie, Sung-Ja; Ryu, Ji Young; Bae, Seunghee; Kim, Young-Sam

doi:10.20402/ajbc.2017.0177

Cited by 5 publications

(2 citation statements)

References 24 publications

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“…SOD 1 is the representative antioxidant enzyme among all SOD enzymes. It can be activated by different substances, such as genoposidic acid [ 72 ], sinapic acid [ 73 ], or nicotinyl–isoleucine–valine–histidine [ 64 ]. However, other promising compounds such as graphene oxide, are not able to upregulate the SOD1 gene using non-cytotoxic concentrations [ 74 ].…”

Section: Resultsmentioning

confidence: 99%

Zinc Chloride: Time-Dependent Cytotoxicity, Proliferation and Promotion of Glycoprotein Synthesis and Antioxidant Gene Expression in Human Keratinocytes

Salesa

Serra

Serrano‐Aroca

2021

Biology

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The use of ionic metals such as zinc (Zn2+) is providing promising results in regenerative medicine. In this study, human keratinocytes (HaCaT cells) were treated with different concentrations of zinc chloride (ZnCl2), ranging from 1 to 800 µg/mL, for 3, 12 and 24 h. The results showed a time–concentration dependence with three non-cytotoxic concentrations (10, 5 and 1 µg/mL) and a median effective concentration value of 13.5 µg/mL at a cell exposure to ZnCl2 of 24 h. However, the zinc treatment with 5 or 1 µg/mL had no effect on cell proliferation in HaCaT cells in relation to the control sample at 72 h. The effects of the Zn2+ treatment on the expression of several genes related to glycoprotein synthesis, oxidative stress, proliferation and differentiation were assessed at the two lowest non-cytotoxic concentrations after 24 h of treatment. Out of 13 analyzed genes (superoxide dismutase 1 (SOD1), catalase (CAT), matrix metallopeptidase 1 (MMP1), transforming growth factor beta 1 (TGFB1), glutathione peroxidase 1 (GPX1), fibronectin 1 (FN1), hyaluronan synthase 2 (HAS2), laminin subunit beta 1 (LAMB1), lumican (LUM), cadherin 1 (CDH1), collagen type IV alpha (COL4A1), fibrillin (FBN) and versican (VCAN)), Zn2+ was able to upregulate SOD1, CAT, TGFB1, GPX1, LUM, CDH1, FBN and VCAN, with relative expression levels of at least 1.9-fold with respect to controls. We found that ZnCl2 promoted glycoprotein synthesis and antioxidant gene expression, thus confirming its great potential in biomedicine.

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Section: Resultsmentioning

confidence: 99%

Zinc Chloride: Time-Dependent Cytotoxicity, Proliferation and Promotion of Glycoprotein Synthesis and Antioxidant Gene Expression in Human Keratinocytes

Salesa

Serra

Serrano‐Aroca

2021

Biology

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“…Recently, Xian et al (2019) have reported that UV irradiation significantly elevates the level of ROS, however, nuclear factor erythroid 2-related factor 2 (Nrf2) overexpression in the skin-derived precursors (SKP) significantly ameliorates UV-induced damage in mice via the remarkable elevation of SOD, CAT, GPx, and reduced glutathione mRNA and protein levels, through Nrf2/HO-1 and PI3K/Akt pathways in a three-dimensional skin model [ 32 ]. In addition, it has been reported that the anti-photoaging effects of natural antioxidants, such as sinapic acid [ 33 ] and juglanin [ 34 ], are mediated by decrease of ROS generation and increases of SOD1, SOD2, CAT, GPx, and Nrf2 gene expression levels in UVB irradiation-damaged HaCaT keratinocytes. Based on the results of the previous studies and our current study, it is likely that LA pretreatment can prevent or attenuate skin damage, by decreasing oxidative stress and increasing antioxidant enzyme levels in mouse dorsal skin.…”

Section: Discussionmentioning

confidence: 99%

Laminarin Attenuates Ultraviolet-Induced Skin Damage by Reducing Superoxide Anion Levels and Increasing Endogenous Antioxidants in the Dorsal Skin of Mice

et al. 2020

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A number of studies have demonstrated that marine carbohydrates display anti-oxidant, anti-melanogenic, and anti-aging activities in the skin. Laminarin (LA), a low-molecular-weight polysaccharide, is found in brown algae. The benefits of LA in ultraviolet B (UVB) induced photodamage of the skin have not been reported. The aim of this study was to investigate the effects of pre-treated LA on histopathological changes and oxidative damage in mouse dorsal skin on day 5, following repeated UVB exposure. Histopathology, Western blot analysis and immunohistochemical studies showed that epidermal thickness in the UVB group was significantly increased; however, the thickness in the UVB group treated with LA (LA/UVB group) was less compared with that of the UVB group. Collagen fibers in the dermis of the UVB group were significantly decreased and destroyed, whereas, in the LA/UVB group, the density of collagen fibers was significantly increased compared with that of the UVB group. Oxidative stress due to superoxide anion production measured via dihydroethidium fluorescence staining was dramatically increased in the UVB group, whereas in the LA/UVB group, the oxidative stress was significantly decreased. Expressions of SOD1, glutathione peroxidase and catalase were markedly reduced in the UVB group, whereas in the LA/UVB group, they were significantly higher along with SOD2 than in the control group. Taken together, our results indicate that LA pretreatment prevents or attenuates skin damage, by decreasing oxidative stress and increasing antioxidant enzymes in mouse dorsal skin.

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Potential Antioxidant and Antiphotoaging Effects of Fagopyrum esculentum Honey on Human Dermal Fibroblasts

Kim¹,

An²,

Bae³

2022

Asian J Beauty Cosmetol

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Purpose: The present study investigated the potential use of Fagopyrum esculentum (F. esculentum) honey as a functional skin cosmetic by examining its antioxidant and antiaging effects on human dermal fibroblasts (HDFs).Methods: Total polyphenol content, total flavonoid content, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity of F. esculentum honey were analyzed. HDF cell viability was examined after treatment with F. esculentum honey and expression of genes encoding collagen and matrix metalloproteinases (MMPs) was measured by quantitative real-time polymerase chain reaction (qRT-PCR) analysis.Results: Analysis of antioxidant activity showed that total polyphenol content, total flavonoid content, and DPPH radical scavenging activity of F. esculentum honey increased in a dose-dependent manner. qRT-PCR analysis revealed an increase in mRNA expression of the collagen-producing genes, collagen type I alpha 1 chain (COL1A1) and collagen type III alpha 1 chain (COL3A1), whereas MMP1 and MMP3 expression decreased in HDFs treated with F. esculentum honey. Furthermore, F. esculentum honey protected HDFs from ultraviolet (UV) irradiation-induced cytotoxicity and reduced reactive oxygen species production. Expression of COL1A1 and COL3A1, which was reduced by UV irradiation, was restored in a dose-dependent manner, whereas expression of MMP1 and MMP3, which was increased by UV irradiation, was reduced in a dose-dependent manner in HDFs pretreated with F. esculentum honey.Conclusion: These findings indicate that F. esculentum honey is a natural antioxidant that is enriched in phenols and flavonoids and has the potential to act as an antiaging cosmetic ingredient due to its protective effects on UV irradiation-damaged skin cells.

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Anti-oxidant and Anti-inflammatory Effects of Sinapic Acid in UVB Irradiation-Damaged HaCaT Keratinocytes

Cited by 5 publications

References 24 publications

Zinc Chloride: Time-Dependent Cytotoxicity, Proliferation and Promotion of Glycoprotein Synthesis and Antioxidant Gene Expression in Human Keratinocytes

Zinc Chloride: Time-Dependent Cytotoxicity, Proliferation and Promotion of Glycoprotein Synthesis and Antioxidant Gene Expression in Human Keratinocytes

Laminarin Attenuates Ultraviolet-Induced Skin Damage by Reducing Superoxide Anion Levels and Increasing Endogenous Antioxidants in the Dorsal Skin of Mice

Potential Antioxidant and Antiphotoaging Effects of Fagopyrum esculentum Honey on Human Dermal Fibroblasts

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