Anti-oxidant and Anti-inflammatory Effects of Chulbu-tang

Abstract

Objectives Even though the various alternative herbal medicine has applied for osteoarthritis (OA) treatment, its scientific proof remains uncertain. The aim of the present study evaluates the effects of Chulbu-tang on inflammatory responses in a monosodium iodoacetate (MIA)-induced osteoarthritis rat model. Methods OA rat model was established by MIA injection in intra-joint of rats. 7 days after, OA rats except OA control rats were administrated Chulbu-tang (100 or 200 mg/kg) or Indomathacin (5 mg/kg) once a day for 14 days. The weight-bearing ability of hind paws were measured when group isolation 0, 7, and 14 days. Western blotting was performed to examine the knockdown/overexpressing efficiency of Chulbu-tang. In addition, cartilage destruction was measured histologically. Results Chulbu-tang treatment significantly reduced the protein expressions of inflammatory mediators such as inducible nitric oxide synthase and cyclooxygenase 2, and inhibited inflammatory cytokines including tumor necrosis factor alpha, interleukin (IL)-1β, and IL-6 through nuclear factor-kappa B (NF-κB) inactivation. Moreover, anti-oxidant enzymes such as superoxide dismutase and glutathione peroxidase-1/2 through nuclear factor-erythroid 2-related factor 2 (Nrf2) pathway significantly increased. Our findings indicate that Chulbu-tang has the potential therapeutic effect on OA through inhibiting the inflammatory responses via inactivating NF-κB signaling pathway. In addition, upregulation of Nrf2 led to anti-oxidant effects. Conclusions Taken together, Chulbu-tang is believed to have antioxidant, anti-inflammatory effects, and cartilage protection for arthritis-causing rats.

Effects of Sunbanghwalmyung-Eum Gamibang on MIA-Induced Osteoarthritis in Rats

“…. 특히, 골관절염에 대한 한약 치료에 효과에 대해서는 大羌活湯 3) , 朮附湯 4) , 知栢地黃湯 5) , 三氣飮 6) , 疏風活血湯 7) 및 大防風湯 8) 등의 처 방을 활용한 연구 결과가 보고되었으나, 항염증 효과가 있는 것으 로 알려져 膝腫痛에 사용할 수 있는 仙方活命飮에 대한 구체적인 연구는 아직 보고된 바가 없다.…”