Anti-Oxidant and Pro-Oxidant Effects of Peroxiredoxin 6: A Potential Target in Respiratory Diseases

Jia, Wenhui; Dong, Chunling; Li, Bo

doi:10.3390/cells12010181

Cited by 10 publications

(4 citation statements)

References 94 publications

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“…The activation of both transcription factors was corroborated by the enhanced levels of Ifnb and Cxcl10, downstream of both pathways (Figure 2F). Finally, we found that alterations in the IRI group were associated with a decrease in Prdx6 (Figure 2G), an antioxidant protein known to protect against oxidative stress [32,33]. Taken together, these findings suggest that PM 2.5 exposure and IRI act synergistically to enhance inflammation by activating the cGAS–STING pathway, possibly via compromised mitochondrial integrity and oxidative stress in the damaged tubular epithelium.…”

Section: Resultsmentioning

confidence: 78%

Air pollution aggravates renal ischaemia–reperfusion‐induced acute kidney injury

Sanches,

Parra,

Sun

et al. 2024

The Journal of Pathology

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Chronic kidney disease (CKD) has emerged as a significant global public health concern. Recent epidemiological studies have highlighted the link between exposure to fine particulate matter (PM2.5) and a decline in renal function. PM2.5 exerts harmful effects on various organs through oxidative stress and inflammation. Acute kidney injury (AKI) resulting from ischaemia–reperfusion injury (IRI) involves biological processes similar to those involved in PM2.5 toxicity and is a known risk factor for CKD. The objective of this study was to investigate the impact of PM2.5 exposure on IRI‐induced AKI. Through a unique environmentally controlled setup, mice were exposed to urban PM2.5 or filtered air for 12 weeks before IRI followed by euthanasia 48 h after surgery. Animals exposed to PM2.5 and IRI exhibited reduced glomerular filtration, impaired urine concentration ability, and significant tubular damage. Further, PM2.5 aggravated local innate immune responses and mitochondrial dysfunction, as well as enhancing cyclic GMP–AMP synthase‐stimulator of interferon genes (cGAS–STING) pathway activation. This increased renal senescence and suppressed the anti‐ageing protein klotho, leading to early fibrotic changes. In vitro studies using proximal tubular epithelial cells exposed to PM2.5 and hypoxia/reoxygenation revealed heightened activation of the STING pathway triggered by cytoplasmic mitochondrial DNA, resulting in increased tubular damage and a pro‐inflammatory phenotype. In summary, our findings imply a role for PM2.5 in sensitising proximal tubular epithelial cells to IRI‐induced damage, suggesting a plausible association between PM2.5 exposure and heightened susceptibility to CKD in individuals experiencing AKI. Strategies aimed at reducing PM2.5 concentrations and implementing preventive measures may improve outcomes for AKI patients and mitigate the progression from AKI to CKD. © 2024 The Authors. The Journal of Pathology published by John Wiley & Sons Ltd on behalf of The Pathological Society of Great Britain and Ireland.

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Section: Resultsmentioning

confidence: 78%

Air pollution aggravates renal ischaemia–reperfusion‐induced acute kidney injury

Sanches,

Parra,

Sun

et al. 2024

The Journal of Pathology

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“…The aiPLA2 activity has been confirmed in the deduced amino acids from the nucleotide sequences of clones isolated from a cDNA library of human adult myeloid cells [17,25]. The catalytic triad (S32, H26, and D140) of phospholipase activity resides on the surface of the Prdx6 protein, which plays a pivotal role in the reduction of oxidized phospholipids and cell-membrane remodeling [26]. Research has indicated that the mutations in the His26 and Ser32 of Prdx6 lead to the loss of the ability to bind phospholipids and the aiPLA2 activity.…”

Section: Enzyme Activities and Function Of Prdx6mentioning

confidence: 85%

Unveiling the Significance of Peroxiredoxin 6 in Central Nervous System Disorders

Xue,

Huang,

Zhu

et al. 2024

Antioxidants

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Peroxiredoxin 6 (Prdx6), a unique 1-Cys member of the peroxiredoxin family, exhibits peroxidase activity, phospholipase activity, and lysophosphatidylcholine acyltransferase (LPCAT) activity. Prdx6 has been known to be an important enzyme for the maintenance of lipid peroxidation repair, cellular metabolism, inflammatory signaling, and antioxidant damage. Growing research has demonstrated that the altered activity of this enzyme is linked with various pathological processes including central nervous system (CNS) disorders. This review discusses the distinctive structure, enzyme activity, and function of Prdx6 in different CNS disorders, as well as emphasizing the significance of Prdx6 in neurological disorders.

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“…The antioxidant enzymes involved in cellular defence, such as CAT, SOD, PRDX and GPx, are responsible for converting superoxide radicals into water (Sovernigo et al., 2017). The SOD converts the superoxide radical into hydrogen peroxide and oxygen, while CAT, GPx and PRDX convert these radicals into water (Baszyński et al., 2022; Jia et al., 2023).…”

Section: Introductionmentioning

confidence: 99%

“…The SOD converts the superoxide radical into hydrogen peroxide and oxygen, while CAT, GPx and PRDX convert these radicals into water (Baszyński et al, 2022;Jia et al, 2023).…”

Section: Introductionmentioning

confidence: 99%

Melatonin improves the viability and ultrastructure of bovine oocyte‐granulosa complexes of in vitro cultured early antral follicles

Silva,

Nascimento,

Costa

et al. 2024

Reprod Domestic Animals

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This study aims to investigate the effects of melatonin on follicular growth, viability and ultrastructure, as well as on the levels of mRNA for antioxidant enzymes, reactive oxygen species (ROS) and meiotic progression in oocytes from in vitro cultured bovine early antral follicles. To this end, isolated early antral follicles (500–600 μm) were cultured in TCM‐199+ alone or supplemented with 10−6, 10−7 or 10−8 M melatonin at 38.5°C with 5% CO2 for 8 days. Follicle diameters were evaluated at days 0, 4 and 8 of culture. At the end of culture, ultrastructure, chromatin configuration, viability (calcein‐AM and ethidium homodimer‐1 staining), and the levels of ROS and mRNA for catalase (CAT), superoxide dismutase (SOD) and peroxiredoxin 6 (PRDX6) and glutathione peroxidase (GPx) were investigated in oocyte‐granulosa cell complexes (OGCs). The results showed that early antral follicles cultured with 10−6 and 10−8 M melatonin had a progressive and significant increase in their diameters throughout the culture period (p < .05). Additionally, oocytes from follicles cultured with 10−7 or 10−8 M melatonin had increased fluorescence for calcein‐AM, while those cultured with 10−6 or 10−7 M had reduced fluorescence for ethidium homodimer‐1. Different from follicles cultured in other treatments, those cultured with 10−8 M melatonin had well‐preserved ultrastructure of oocyte and granulosa cells. Melatonin, however, did not influence the levels of ROS, the mitochondrial activity, oocyte meiotic resumption and expression mRNA for SOD, CAT, GPX1 and PRDX6. In conclusion, the presence of 10−8 M melatonin in culture medium improves viability and preserves the ultrastructure of oocyte and granulosa cells of early antral follicles cultured in vitro.

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Anti-Oxidant and Pro-Oxidant Effects of Peroxiredoxin 6: A Potential Target in Respiratory Diseases

Cited by 10 publications

References 94 publications

Air pollution aggravates renal ischaemia–reperfusion‐induced acute kidney injury

Air pollution aggravates renal ischaemia–reperfusion‐induced acute kidney injury

Unveiling the Significance of Peroxiredoxin 6 in Central Nervous System Disorders

Melatonin improves the viability and ultrastructure of bovine oocyte‐granulosa complexes of in vitro cultured early antral follicles

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