Anti-oxidative and TNF-α suppressive activities of puerarin derivative (4AC) in RAW264.7 cells and collagen-induced arthritic rats

Xiao, Cheng; Li, Jian; Dong, Xinxin; He, Xiaojuan; Niu, Xuyan; Cha, Liu; Zhong, Guoyue; Bauer, Rudolf; Yang, Da; Lü, Aiping

doi:10.1016/j.ejphar.2011.05.061

Cited by 62 publications

(41 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Puerarin intervention inhibited the changes in the numbers of microglia and neurons as well as secretion of TNF-α and IL-1β. Therefore, we speculate that the protective effect of puerarin against damage caused by alcohol poisoning may be related mainly to the anti-inflammatory activity of puerarin (33), which reduces the activation of microglia and subsequent damage to or death of neurons, resulting in protection of spatial learning and memory ability.…”

Section: Discussionmentioning

confidence: 99%

Puerarin protects against damage to spatial learning and memory ability in mice with chronic alcohol poisoning

Cui

Wang

Zheng

et al. 2015

Braz J Med Biol Res

View full text Add to dashboard Cite

We evaluated the effect of puerarin on spatial learning and memory ability of mice with chronic alcohol poisoning. A total of 30 male C57BL/6 mice were randomly divided into model, puerarin, and control groups (n=10 each). The model group received 60% (v/v) ethanol by intragastric administration followed by intraperitoneal injection of normal saline 30 min later. The puerarin group received intragastric 60% ethanol followed by intraperitoneal puerarin 30 min later, and the control group received intragastric saline followed by intraperitoneal saline. Six weeks after treatment, the Morris water maze and Tru Scan behavioral tests and immunofluorescence staining of cerebral cortex and hippocampal neurons (by Neu-N) and microglia (by Ib1) were conducted. Glutamic acid (Glu) and gamma amino butyric acid (GABA) in the cortex and hippocampus were assayed by high-performance liquid chromatography (HPLC), and tumor necrosis factor (TNF)-α and interleukin (IL)-1β were determined by ELISA. Compared with mice in the control group, escape latency and distance were prolonged, and spontaneous movement distance was shortened (P<0.05) by puerarin. The number of microglia was increased in both the cortex and hippocampal dentate gyrus (P<0.01), and neurons were reduced only in the hippocampal dentate gyrus (P<0.01) in puerarin-treated mice. In the model group, Glu and GABA levels decreased (P<0.05), and Glu/GABA, TNF-α, and IL-1β increased (P<0.01) with puerarin treatment, returning to near normal levels. In conclusion, puerarin protected against the effects of chronic alcohol poisoning on spatial learning and memory ability primarily because of anti-inflammatory activity and regulation of the balance of Glu and GABA.

show abstract

Section: Discussionmentioning

confidence: 99%

Puerarin protects against damage to spatial learning and memory ability in mice with chronic alcohol poisoning

Cui

Wang

Zheng

et al. 2015

Braz J Med Biol Res

View full text Add to dashboard Cite

show abstract

“…H 2 DCF-DA can pass through the cell membrane and oxidized by ROS to form the fluorochrome 2′,7′dichlorofluorescein (DCF). Therefore, H 2 DCF-DA was widely used to reflect the intracellular ROS content [42–44]. The treated cells were collected, washed twice with ice cold PBS, and incubated with H 2 DCF-DA (20 μ M) in the dark at 37°C for 15 min.…”

Section: Methodsmentioning

confidence: 99%

The Aqueous Extract of Rhizome ofGastrodia elataProtectedDrosophilaand PC12 Cells against Beta-Amyloid-Induced Neurotoxicity

Koon

et al. 2013

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

This study aims to investigate the neuroprotective effect of the rhizome of Gastrodia elata (GE) aqueous extract on beta-amyloid(Aβ)-induced toxicity in vivo and in vitro. Transgenic Drosophila mutants with Aβ-induced neurodegeneration in pan-neuron and ommatidia were used to determine the efficacy of GE. The antiapoptotic and antioxidative mechanisms of GE were also studied in Aβ-treated pheochromocytoma (PC12) cells. In vivo studies demonstrated that GE (5 mg/g Drosophila media)-treated Drosophila possessed a longer lifespan, better locomotor function, and less-degenerated ommatidia when compared with the Aβ-expressing control (all P < 0.05). In vitro studies illustrated that GE increased the cell viability of Aβ-treated PC12 cells in dose-dependent manner, probably through attenuation of Aβ-induced oxidative and apoptotic stress. GE also significantly upregulated the enzymatic activities of catalase, superoxide dismutase, and glutathione peroxidase, leading to the decrease of reactive oxidation species production and apoptotic marker caspase-3 activity. In conclusion, our current data presented the first evidence that the aqueous extract of GE was capable of reducing the Aβ-induced neurodegeneration in Drosophila, possibly through inhibition of apoptosis and reduction of oxidative stress. GE aqueous extract could be developed as a promising herbal agent for neuroprotection and novel adjuvant therapies for Alzheimer's disease.

show abstract

“…1A) (18). Puerarin has been shown to exhibit therapeutic effects on NAFLD by acting as an antioxidant, lowering cholesterol levels and improving leptin signal transduction (19,20).…”

Section: Introductionmentioning

confidence: 99%

Comparison between the efficacies of curcumin and puerarin in C57BL/6 mice with steatohepatitis induced by a methionine- and choline-deficient diet

Wang

Zhuge

et al. 2013

Experimental and Therapeutic Medicine

Self Cite

View full text Add to dashboard Cite

Non-alcoholic fatty liver disease (NAFLD) is a prevalent disease, which features an abnormal accumulation of lipids inside hepatocytes. Steatohepatitis plays a critical role in the process resulting in liver fibrosis and cirrhosis. Curcumin and puerarin are herbal products widely used in Asia, which are believed to have therapeutic benefits for alleviating the symptoms of steatohepatitis. In this study, mice models of steatohepatitis induced by a methionine- and choline-deficient diet (MCD) were established to compare the pharmacological actions of curcumin and puerarin. The results showed that curcumin and puerarin exerted inhibitory effects against MCD-induced steatohepatitis in mice. Briefly, curcumin and puerarin significantly downregulated the levels of tumor necrosis factor-α in the blood serum of mice (P<0.01, versus the MCD group). In addition, the levels of triglycerides, total cholesterol and low density lipoproteins in the serum were significantly reduced by puerarin treatment (P<0.05, versus the MCD group). The concentration of interleukin-6 was downregulated by curcumin only (P<0.01, versus the MCD group). Curcumin and puerarin significantly increased the levels of peroxisome proliferator-activated receptor-γ (PPARγ; P<0.05, versus the MCD group). Moreover, increased nuclear factor-κB (NF-κB) was markedly attenuated by curcumin (P<0.05, versus the MCD group). In conclusion, curcumin and puerarin appear to exert different actions against steatohepatitis. It is possible that puerarin regulated lipid metabolism in the ‘first hit’ stage through the PPARγ pathway, while curcumin inhibited the inflammatory response in the ‘second hit’ stage through the NF-κB pathway.

show abstract

Anti-oxidative and TNF-α suppressive activities of puerarin derivative (4AC) in RAW264.7 cells and collagen-induced arthritic rats

Cited by 62 publications

References 30 publications

Puerarin protects against damage to spatial learning and memory ability in mice with chronic alcohol poisoning

Puerarin protects against damage to spatial learning and memory ability in mice with chronic alcohol poisoning

The Aqueous Extract of Rhizome ofGastrodia elataProtectedDrosophilaand PC12 Cells against Beta-Amyloid-Induced Neurotoxicity

Comparison between the efficacies of curcumin and puerarin in C57BL/6 mice with steatohepatitis induced by a methionine- and choline-deficient diet

Contact Info

Product

Resources

About