The Aqueous Extract of Rhizome of<i>Gastrodia elata</i>Protected<i>Drosophila</i>and PC12 Cells against Beta-Amyloid-Induced Neurotoxicity

Ng, CF; Ko, Chun‐Hay; Koon, Chi Man; Xian, Jia-Wen; Leung, Ping‐Chung; Fung, Kwok‐Pui; Chan, Ho Yin Edwin; Lau, Clara Bik‐San

doi:10.1155/2013/516741

Cited by 32 publications

(24 citation statements)

References 80 publications

(85 reference statements)

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“…Treatment with specific chelators or overexpression of metal responsive transcription factor 1 (MTF-1) or metallothionein A (MtnA), two detoxifying proteins, prevented the toxicity of copper and zinc. The role of copper in Aβ42 toxicity was further confirmed in studies modulating the activity of high-affinity copper importers (Ctr1A, B, and C) and exporters (ATP7, Atox1), and FKBP56, an immunophilin though to modulate the levels of intracellular copper (Lang et al, 2013; Sanokawa-Akakura et al, 2010). Interestingly, Ctr1C loss-of-function increased the insolubility of Aβ42, suggesting that both iron and copper promote Aβ42 aggregation into less toxic amyloid fibers.…”

Section: Drosophila Models Of Aβ42 Neurotoxicitymentioning

confidence: 78%

Modeling the complex pathology of Alzheimer's disease in Drosophila

Fernández-Fúnez

Mena

Rincón-Limas

2015

Experimental Neurology

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Alzheimer’s disease (AD) is the leading cause of dementia and the most common neurodegenerative disorder. AD is mostly a sporadic disorder and its main risk factor is age, but mutations in three genes that promote the accumulation of the amyloid-β (Aβ42) peptide revealed the critical role of Amyloid precursor protein (APP) processing in AD. Neurofibrillary tangles enriched in tau are the other pathological hallmark of AD, but the lack of causative tau mutations still puzzles researchers. Here, we describe the contribution of a powerful invertebrate model, the fruit fly Drosophila melanogaster, to uncovering the function and pathogenesis of human APP, Aβ42, and tau. APP and tau participate in many complex cellular processes, although their main function is microtubule stabilization and the to-and-fro transport of axonal vesicles. Additionally, expression of secreted Aβ42 induces prominent neuronal death in Drosophila, a critical feature of AD, making this model a popular choice for identifying intrinsic and extrinsic factors mediating Aβ42 neurotoxicity. Overall, Drosophila has made significant contributions to better understand the complex pathology of AD, although additional insight can be expected from combining multiple transgenes, performing genome-wide loss-of-function screens, and testing anti-tau therapies alone or in combination with Aβ42.

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Section: Drosophila Models Of Aβ42 Neurotoxicitymentioning

confidence: 78%

Modeling the complex pathology of Alzheimer's disease in Drosophila

Fernández-Fúnez

Mena

Rincón-Limas

2015

Experimental Neurology

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“…Fresh rhizomes of H. rostrata were collected, washed in tap water to get rid of any contaminants [13] and then dried to remove water from the surface of the rhizomes. The rhizomes were cut into small pieces and dried under shade at room temperature for 7 d. The dried rhizomes were ground to a coarse powder [14] and defatted using petroleum ether.…”

Section: Methodsmentioning

confidence: 99%

Quantitative Phytochemical Estimation and Evaluation of Antioxidant and Antibacterial Activity of Methanol and Ethanol Extracts of Heliconia rostrata

Moonmun¹

2017

ijps

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Moonmun, et al.: Antioxidant and Antibacterial Activity of Heliconia rostrataRhizomes of Heliconia rostrate, (family: Heliconiaceae) have been well known for antiophidic property. Ethnobotanically, rhizome of this plant has also been used to cure jaundice, intestinal pain and hypertension. This study aimed at evaluating the antioxidant and antimicrobial potency of two extracts of rhizomes of H. rostrata to find out the one, which exhibited better activity. Methanol and ethanol extract of the dried and defatted rhizomes were prepared and were subjected to qualitative and quantitative estimation of phytoconstituents, antioxidant assay and antibacterial evaluation. The methanol extract was found to give better yield than the ethanol extract. All phytoconstituents detected in the methanol extract were found in the ethanol extract also except for the glycoside content, which was found only in the methanol extract. Total phenolic and flavonoid content was higher in the ethanol extract compared to the methanol extract, whereas total tannin content was higher in the methanol extract. Both ethanol and methanol extracts exhibited antioxidant and antibacterial potency. The antioxidant activities of both extracts were found to be equal, while the ethanol extract exerted significantly higher antibacterial activity compared to the methanol extract. Key words: Heliconiaceae, rhizome, phytoconstituents, antioxidant, antimicrobialMedicinal plants offer alternative therapies with spectacular opportunities [1] . Heliconia rostrata, popularly known as false bird of paradise and hanging lobster claw (family: Heliconiaceae) is a shrub that is reported to contain calcium, nitrogen, potassium, magnesium, iron, phosphorous, chlorine, sulphur, sugar, carbohydrate, starch and protein [2] . This plant is reported to be well known for antiophidic property [3] . Ethnobotanically, the traditional people of Malaysia used these rhizomes to cure jaundice, intestinal pain and hypertension [4] . However, not much of experimental evidence was documented.Oxygen, the most important component for the survival of living being is highly reactive and can do serious damage to healthy cells of the body as a free radical. Oxidation produces free radicals, which can start chain reactions in the cell leading to cell damage and even cell death [5] . An antioxidant terminates these radical reactions by removing free radical intermediates, which causes ageing and disease like atherosclerosis, cancer, cardiovascular diseases and CNS disorders [6] thus, play a vital role in inhibiting or delaying the oxidation at the cellular level [7] . Synthetic antioxidants, butylatedhydoxytoluene (BHT), butylatedhydroxyanisole (BHA) and tert-butyl hydroquinone (TBHQ) are being evaluated in many countries for their potential health hazard and have been found to promote liver damage and causing cancer to laboratory animals [8] . In comparison, the plant sources prove to have less or no side effect [6] , thus channelizing the interest towards the nature for antioxidant potency.Re...

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“…Oncovin is the brand name for vincristine, which is used as a chemotherapy drug for many types of cancer. Vincristine is also used to treat neurodegenerative disease (Chun-Fai et al, 2013). The mode of action of vincristine is that it acts on the tubulin protein, thereby inhibiting the metaphase stage, thus leading the cell to apoptosis (Jordan, 2002).…”

Section: Introductionmentioning

confidence: 99%

Antioxidant and cytotoxic activity of Tecoma stans against lung cancer cell line (A549)

Robinson¹,

Kumaresan²,

Subbaiya³

et al. 2017

Braz. J. Pharm. Sci.

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Human have been constantly using plants and plant products to overcome many diseases. The antioxidant property of the plant sources is studied to obtain an efficacious drug against cancer. The objectives of the present study is to evaluate the antioxidant and cytotoxic activity of the Tecoma stans extracts against lung cancer cell line in comparison with vincristine drug. The antioxidant activity was studied using the standard DPPH assay and the cytotoxic activity using MTT assay. DPPH assay results show that methanolic extract of T. stans in higher concentration show better antioxidant potential than the standard L-ascorbic acid. They exhibited strong antioxidant potential at 20 µg/mL concentration. The absorbance at 517 nm showed that in the range of 0.201-0.0203 compared to that of absorbance of ascorbic acid at 0.023.Cytotoxic activity was studied using MTT assay which showed that the increase in concentration of extract increases the cell death. At 100µg/mL concentration there is an increased cytotoxic activity, i.e., 99% of cell inhibition. The results of antioxidant and anticancerous activity may be positively correlated.

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The Aqueous Extract of Rhizome ofGastrodia elataProtectedDrosophilaand PC12 Cells against Beta-Amyloid-Induced Neurotoxicity

Cited by 32 publications

References 80 publications

Modeling the complex pathology of Alzheimer's disease in Drosophila

Modeling the complex pathology of Alzheimer's disease in Drosophila

Quantitative Phytochemical Estimation and Evaluation of Antioxidant and Antibacterial Activity of Methanol and Ethanol Extracts of Heliconia rostrata

Antioxidant and cytotoxic activity of Tecoma stans against lung cancer cell line (A549)

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