Antioxidant and cytotoxic activity of Tecoma stans against lung cancer cell line (A549)

Robinson, J. Philip; Kumaresan, Suriya; Subbaiya, Ramasamy; Ponmurugan, P.

doi:10.1590/s2175-97902017000300204

Cited by 26 publications

(23 citation statements)

References 9 publications

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“…However, it also suggests that this effect is dependent on the kind of solvent used to extract the compounds. In many studies, caspase activity has been shown to be more commonly activated in methanolic extracts [16][17][18][19] . Many in vitro and in vivo studies have shown many medicinal plants as promising for the treatment of cancer cells.…”

Section: Discussionmentioning

confidence: 99%

Anticancer properties of Tulbaghia violacea regulate the expression of p53-dependent mechanisms in cancer cell lines

Motadi

Choene

Mthembu

2020

Sci Rep

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Cancer is an enormous burden of disease globally. Today, more people die from cancer than a combination of several diseases. And in females, breast and cervical malignancies remain the most common types. Currently, cervical and breast cancer are the most diagnosed gynecological cancer type amongst black females in the Southern Sahara while amongst males prostate cancer is on the upward trend. With many of them still dependent on medicinal plants as a form of therapy and the need to identify new therapeutic agents, we have identified a commonly used medicinal plant Tulbaghia violacea Harv. commonly known as Itswele lomlambo (Xhosa), wilde knoffel (Afrikaans) and Isihaqa (zulu) to evaluate its anticancer properties at a molecular biology level. In this study, we evaluated the molecular mechanism of T. violacea extracts in regulating cell death in various cancer cell lines. To achieve this, T. violacea was collected, dried before crushing into a fine ground powder. Three organic solvents namely, methanol, hexane, and butanol at 10 g per 100 mL were used as extraction solvents. Each cell line was treated with varying concentrations of the plant extract to identify the half-maximal inhibitory concentration (IC50). The IC 50 was later used to analyse if the extracts were inducing apoptosis using annexin V analysis. Furthermore, the molecular mechanisms by which apoptosis was induced was analysed by qPCR, western blots. All three extracts exhibited anticancer activity with the most cytotoxic being methanol extract. p53 expression was significantly increased in treated cells that correlated with increased caspase activity. The results point to possible activation of apoptosis following treatment with hexane extracts.

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Section: Discussionmentioning

confidence: 99%

Anticancer properties of Tulbaghia violacea regulate the expression of p53-dependent mechanisms in cancer cell lines

Motadi

Choene

Mthembu

2020

Sci Rep

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“…Methanolic extract of Careya arborea at the concentration of 10 ug/ml and 20 ug/ml exhibited the decreased cell viability, however with a concentration at 40ug/ml showed a non-significant difference. This is possibly due to the fact that, we used a crude methanolic extract of the plant, which contains numerous useful and unusual bioactive compounds [16]. Distilled water extract of the plant showed increased cell viability, suggesting its non-potential to treat A549 cells.…”

Section: Discussionmentioning

confidence: 99%

Screening of Selected Ethno-Medicinal Plants for Anti-Cancer Activity

Dongre¹,

Meshram²,

Dighe³

et al. 2020

azb

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Cancer is an epidemic worldwide. As per the report of the National Centre for Health Statistics in 2019, approximately 606,880 patients were died due to cancer and 1,762,450 new cases were detected. To overcome this scenario, worldwide research is going on to combat this disease. Among these, treatment of cancer through plant phytochemical has attended much prominence in the last few decades. Phytochemicals are produced by plants for their defense mechanism. However, these can also be used to treat many deadly diseases, among them cancer is most common. In the present study, we selected three Ethno-medicinal plants namely Careya arborea (leaf), Ficus religiosa (leaf) and Amorphophallus campanulatus (tuber) to evaluate their efficacy against A549 human lung cancer cells. MTT assay is widely used to assess cell viability. Human lung adenocarcinoma cell lines were purchased from NCCS, Pune and maintained in the laboratory under all standard conditions. Cells were grown, using ready to use media containing 4.5 grams of glucose, L-glutamine and sodium pyruvate. Growth factors were made available to the cells through the fetal bovine serum. We treated the cells with different concentrations of methanol and distilled water extracts of the selected plants and investigate the cell viability using a statistical test. We found varied significant differences between control cells and the cells treated with plant extracts.

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“…Tecoma stans was tested against A549 (human lung adenocarcinoma) cell line and cell viability was reduced in a dose-dependent manner, indicating a moderate cytotoxic effect [49]. Another study showed that the plant extract was cytotoxic against HepG2 cells where a reduction in viability of cancer cells was observed [50].…”

Section: Bignoniaceaementioning

confidence: 99%

A Review of Cytotoxic Plants of the Indian Subcontinent and a Broad-Spectrum Analysis of Their Bioactive Compounds

Mazumder

Biswas²,

Raja³

et al. 2020

Molecules

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Cancer or uncontrolled cell proliferation is a major health issue worldwide and is the second leading cause of deaths globally. The high mortality rate and toxicity associated with cancer chemotherapy or radiation therapy have encouraged the investigation of complementary and alternative treatment methods, such as plant-based drugs. Moreover, over 60% of the anti-cancer drugs are molecules derived from plants or their synthetic derivatives. Therefore, in the present review, an attempt has been made to summarize the cytotoxic plants available in the Indian subcontinent along with a description of their bio-active components. The review covers 99 plants of 57 families as well as over 110 isolated bioactive cytotoxic compounds, amongst which at least 20 are new compounds. Among the reported phytoconstituents, artemisinin, lupeol, curcumin, and quercetin are under clinical trials, while brazilin, catechin, ursolic acid, β-sitosterol, and myricetin are under pharmacokinetic development. However, for the remaining compounds, there is little or no information available. Therefore, further investigations are warranted on these subcontinent medicinal plants as an important source of novel cytotoxic agents.

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Antioxidant and cytotoxic activity of Tecoma stans against lung cancer cell line (A549)

Cited by 26 publications

References 9 publications

Anticancer properties of Tulbaghia violacea regulate the expression of p53-dependent mechanisms in cancer cell lines

Anticancer properties of Tulbaghia violacea regulate the expression of p53-dependent mechanisms in cancer cell lines

Screening of Selected Ethno-Medicinal Plants for Anti-Cancer Activity

A Review of Cytotoxic Plants of the Indian Subcontinent and a Broad-Spectrum Analysis of Their Bioactive Compounds

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