Anti-Pancreatic Lipase and Antioxidant Activity of Selected Tropical Herbs

Sahib, Najla Gooda; Hamid, Azizah Abdul; Saari, Nazamid; Abas, Faridah; Dek, Mohd Sabri Pak; Rahim, M S A Abdul

doi:10.1080/10942912.2010.494754

Cited by 21 publications

(9 citation statements)

References 32 publications

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“…Pancreatic and lipoprotein lipase activity was carried out based on previous methods, as described elsewhere [ 7 , 13 ], both titrimetric methods based on the quantification of free fatty acids released in a substrate, lipase and extract system as compared to a substrate and lipase system only.…”

Section: Methodsmentioning

confidence: 99%

“…There has been much effort to develop new anti-obesity agents from natural sources such as medicinal plants. Plant bioactive compounds, also termed phytochemicals, have been reported to possess anti-obesity effects through numerous mechanisms, such as inhibition of digestive and metabolic lipases, suppression of appetite, increase in thermogenesis, and inhibition of pre-adipocytes proliferation and differentiation [ 7 , 8 ]. Several reports on the anti-obesity effect of plant extracts in animal-based models of obesity have been published [ 9 – 12 ].…”

Section: Introductionmentioning

confidence: 99%

“…The potential anti-obesity effect of Morinda citrifolia L. fruit extracts in vitro has been previously reported [ 7 , 13 ]. The leaves, fruits, and roots of M. citrifolia L. are widely used in folk medicine in Southeast Asia, Polynesia, Tahiti, Australia, and Hawaii [ 14 ] and are associated with many health-promoting properties.…”

Section: Introductionmentioning

confidence: 99%

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Morinda citrifoliaL. leaf extract prevent weight gain in Sprague-Dawley rats fed a high fat diet

Jambocus

Ismail

Khatib

et al. 2017

Food & Nutrition Research

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Background: Morinda citrifolia L. is widely used as a folk medicinal food plant to manage a panoply of diseases, though no concrete reports on its potential anti-obesity activity. This study aimed to evaluate the potential of M. citrifolia leaf extracts (MLE60) in the prevention of weight gain in vivo and establish its phytochemical profile.Design: Male Sprague-Dawley rats were divided into groups based on a normal diet (ND) or high fat diet (HFD), with or without MLE60 supplementation (150 and 350 mg/kg body weight) and assessed for any reduction in weight gain. Plasma leptin, insulin, adiponectin, and ghrelin of all groups were determined. 1H NMR and LCMS methods were employed for phytochemical profiling of MLE60.Results: The supplementation of MLE60 did not affect food intake indicating that appetite suppression might not be the main anti-obesity mechanism involved. In the treated groups, MLE60 prevented weight gain, most likely through an inhibition of pancreatic and lipoprotein activity with a positive influence on the lipid profiles and a reduction in LDL levels . MLE60 also attenuated visceral fat deposition in treated subjects with improvement in the plasma levels of obesity-linked factors . 1Spectral analysis showed the presence of several bioactive compounds with rutin being more predominant.Conclusion: MLE60 shows promise as an anti-obesity agents and warrants further research.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Morinda citrifoliaL. leaf extract prevent weight gain in Sprague-Dawley rats fed a high fat diet

Jambocus

Ismail

Khatib

et al. 2017

Food & Nutrition Research

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“…Lipase activity was determined by the modified method of Gooda Sahib et al [28]. Porcine lipase was dissolved in 0.01 M Tris-HCl buffer (25 units / mL).…”

Section: Methodsmentioning

confidence: 99%

The Effects of Biopolymer Encapsulation on Total Lipids and Cholesterol in Egg Yolk during in Vitro Human Digestion

Hur

Kim

Choi

et al. 2013

IJMS

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The purpose of this study was to examine the effect of biopolymer encapsulation on the digestion of total lipids and cholesterol in egg yolk using an in vitro human digestion model. Egg yolks were encapsulated with 1% cellulose, pectin, or chitosan. The samples were then passed through an in vitro human digestion model that simulated the composition of mouth saliva, stomach acid, and the intestinal juice of the small intestine by using a dialysis tubing system. The change in digestion of total lipids was monitored by confocal fluorescence microscopy. The digestion rate of total lipids and cholesterol in all egg yolk samples dramatically increased after in vitro human digestion. The digestion rate of total lipids and cholesterol in egg yolks encapsulated with chitosan or pectin was reduced compared to the digestion rate of total lipids and cholesterol in other egg yolk samples. Egg yolks encapsulated with pectin or chitosan had lower free fatty acid content, and lipid oxidation values than samples without biopolymer encapsulation. Moreover, the lipase activity decreased, after in vitro digestion, in egg yolks encapsulated with biopolymers. These results improve our understanding of the effects of digestion on total lipids and cholesterol in egg yolk within the gastrointestinal tract.

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“…In the search for PL inhibitors from natural resources, the identification and characterization of the active phyto-constituents are necessary as therapeutic alternatives to treat obesity. Although the anti-pancreatic lipase activity of M. charantia fruit has been reported earlier (Sahib et al, 2012), the mode and type of inhibition are unknown to date. However, there is still an increasing interest in identifying bioactive constituents present in the fraction of M. charantia.…”

mentioning

confidence: 96%

Study of pancreatic lipase inhibition kinetics and LC–QTOF–MS‐based identification of bioactive constituents of Momordica charantia fruits

Chanda

Mukherjee

Biswas

et al. 2019

Biomedical Chromatography

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The different parts of Momordica charantia have been reported to have several therapeutic applications against hyperglycemia and hypercholesterolemia associated with pancreatic lipase (PL). Inhibition of this enzyme prevents the absorption of dietary triglyceride in the intestine, and thus exerts an anti‐obesity effect. This study aimed to investigate the bioactive constituents of the fruits of M. charantia (MCF) extract and fractions against pancreatic PL followed by study of their inhibition kinetics. The PL inhibitory assay was performed spectrophotometrically by measuring the change in absorbance of the products at 405 nm, using p‐nitrophenylcaprylate as substrate. The results indicated that the ethyl acetate fraction of MCF (EFMC) offered significant, dose‐dependent inhibition against PL, compared with the positive control, Orlistat. The enzyme kinetics study revealed the inhibition to be a mixed type in nature. Additionally, the total phenol and flavonoid content of the fractions was estimated. A positive correlation between phenolic content of EFMC and its PL inhibitory activity was established statistically, which implied that higher inhibition potential was contributed by the phenolic compounds. The identification of the bioactive constituents was further confirmed by LC–QTOF–MS study. This finding suggested that phenolic compounds of MCF can serve as functional food components to address obesity‐related disorders linked with PL.

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Anti-Pancreatic Lipase and Antioxidant Activity of Selected Tropical Herbs

Cited by 21 publications

References 32 publications

Morinda citrifoliaL. leaf extract prevent weight gain in Sprague-Dawley rats fed a high fat diet

Morinda citrifoliaL. leaf extract prevent weight gain in Sprague-Dawley rats fed a high fat diet

The Effects of Biopolymer Encapsulation on Total Lipids and Cholesterol in Egg Yolk during in Vitro Human Digestion

Study of pancreatic lipase inhibition kinetics and LC–QTOF–MS‐based identification of bioactive constituents of Momordica charantia fruits

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