Anti-Parkinson’s Disease Activity of Sanghuangprous vaninii Extracts in the MPTP-Induced Zebrafish Model

Abstract

Parkinson’s disease (PD) is a devastating disease of the central nervous system that occurs mainly in the elderly age group, affecting their quality of life. The PD pathogenesis is not yet fully understood and lacks the disease-modifying treatment strategies. Sanghuangprous vaninii (S. vaninii) is a perennial fungus with a plethora of pharmacological activities including anti-cancer and antioxidant activity and so on. However, no study till date has reported its neuroprotective effect against symptoms that are similar to PD in pre-clinical investigation. In the current study, we investigated anti-PD-like effects of S. vaninii mycelium extracts (SvMEs) on MPTP-induced PD in zebrafish. We observed that the loss of dopaminergic neurons and neurovascular reduction were reversed by using SvMEs in the zebrafish brain in a concentration-independent manner. Moreover, it also relieved locomotor impairments in MPTP-induced PD zebrafish. In addition, SvMEs exerted significant antioxidant activity in vitro, which was also demonstrated in vivo on ktr4:NTR-hKikGR zebrafish. Upon investigating the underlying mechanism, we found that SvMEs may alleviate oxidant stress and accelerate α-synuclein degradation and then alleviate PD-like symptoms. Antioxidant-related genes (sod1, gss, gpx4a, gclm, and cat) implied that the SvMEs exhibited anti-PD activity due to the antioxidation mechanism. Finally, upon analysis of chemical composition of SvMEs by liquid chromatography–mass spectrometry, we identified 10 compounds that are plausibly responsible for the anti-PD-like effect of SvMEs. On the limiting part, the finding of the study would have been more robust had we investigated the protein expression of genes related to PD and oxidative stress and compared the effects of SvMEs with any standard anti-PD therapy. Despite this, our results indicated that SvMEs possess anti-PD effects, indicating SvMEs as a potential candidate that is worth exploring further in this avenue.

The molecular mechanism of three novel peptides from C-phycocyanin alleviates MPTP-induced Parkinson's disease-like pathology in zebrafish

“…To evaluate the neuroprotective effect of active peptides on Parkinson's disease, the PD models of zebrafish were established using MPTP at concentrations of 70 μM and incubated for 72 h, respectively. 32 In addition, we detected the loss of DA neurons and blood vessels using transgenic zebrafish vmat2:GFP and fli1:GFP . Results showed that the DA neuron length of MPTP-treated zebrafish decreased significantly compared with the control.…”

Evaluation of Bioactive Effects of Five Plant Extracts with Different Phenolic Compositions against Different Therapeutic Targets

“…In this scenario, there is emerging evidence on the neuroprotective activity of different phenolic compounds present in various plant sources regarding inhibition of acetylcholinesterase (AchE), Aβ aggregation, proinflammatory markers, and 1-methyl-4phenyl-1-1,2,3,6-tetrahydropyridine (MPTP)-induced apoptosis, among others [12]. Thus, phenolic compounds from different matrices have been shown to have neuroprotective capacity, such as Eucommia ulmoides Oliver (rich in phenolic acids, flavonoids, and iridoid glycosides), herbaceous peony (with paeoninflorin as the main active ingredient), and Sanghuangprous vaninii (an extract rich in caffeic acid); they may ameliorate the dopaminergic deficiency in the MPTP-induced model in the zebrafish brain [12][13][14]. However, common phenolics present in different sources, for example, quercetin, which is contained in different matrices such as apple, chocolate, or cherry [15], have been shown to have both antioxidant and tyrosinase inhibitory actions [16].…”

ErbB2pY‐1248 as a predictive biomarker for Parkinson's disease based on research with RPPA technology and in vivo verification

“…Given the structural similarities between the zebrafish dopamine neuron system and the human striatum, zebrafish has become an excellent model for neurodegenerative and neuropharmacological studies 15,16 . Some studies applied the MPTP‐induced zebrafish PD model to characterize PD‐like cognitive deficits by assessing spatial working memory and spontaneous alternation behavior 17,18 . MPTP‐induced zebrafish PD model is used to study the mitochondria function in the berberine's neuroprotective activity 19 .…”