Anti-Parkinsonian effects of β-amyrin are regulated via LGG-1 involved autophagy pathway in Caenorhabditis elegans

Wei, Chia-Cheng; Chang, Chun‐Han; Liao, Vivian Hsiu‐Chuan

doi:10.1016/j.phymed.2017.09.002

Cited by 44 publications

(18 citation statements)

References 73 publications

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“…We used a previously described protocol to perform the 2′,7′-dichlorodihydrofluorescein diacetate (H 2 DCFDA) assay [ 77 ] for worms with a SpectraMax M2 Microplate Reader (Molecular Devices, Silicon Valley, CA, USA). Briefly, Thirty N2, 6-OHDA-treated or MK/6-OHDA-treated worms, on day 3, were washed with M9 buffer 3 times and transferred to a 96-well plate with 150 μL of PBS per well.…”

Section: Methodsmentioning

confidence: 99%

Maackiain Ameliorates 6-Hydroxydopamine and SNCA Pathologies by Modulating the PINK1/Parkin Pathway in Models of Parkinson’s Disease in Caenorhabditis elegans and the SH-SY5Y Cell Line

Tsai

Liu

et al. 2020

IJMS

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The movement disorder Parkinson’s disease (PD) is the second most frequently diagnosed neurodegenerative disease, and is associated with aging, the environment, and genetic factors. The intracellular aggregation of α-synuclein and the loss of dopaminergic neurons in the substantia nigra pars compacta are the pathological hallmark of PD. At present, there is no successful treatment for PD. Maackiain (MK) is a flavonoid extracted from dried roots of Sophora flavescens Aiton. MK has emerged as a novel agent for PD treatment that acts by inhibiting monoamine oxidase B. In this study, we assessed the neuroprotective potential of MK in Caenorhabditis elegans and investigated possible mechanism of this neuroprotection in the human SH-SY5Y cell line. We found that MK significantly reduced dopaminergic neuron damage in 6-hydroxydopamine (6-OHDA)-exposed worms of the BZ555 strain, with corresponding improvements in food-sensing behavior and life-span. In transgenic worms of strain NL5901 treated with 0.25 mM MK, the accumulation of α-synuclein was diminished by 27% (p < 0.01) compared with that in untreated worms. Moreover, in worms and the SH-SY5Y cell line, we confirmed that the mechanism of MK-mediated protection against PD pathology may include blocking apoptosis, enhancing the ubiquitin-proteasome system, and augmenting autophagy by increasing PINK1/parkin expression. The use of small interfering RNA to downregulate parkin expression in vivo and in vitro could reverse the benefits of MK in PD models. MK may have considerable therapeutic applications in PD.

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Section: Methodsmentioning

confidence: 99%

Maackiain Ameliorates 6-Hydroxydopamine and SNCA Pathologies by Modulating the PINK1/Parkin Pathway in Models of Parkinson’s Disease in Caenorhabditis elegans and the SH-SY5Y Cell Line

Tsai

Liu

et al. 2020

IJMS

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“…We used the transgenic strain BZ555 in the dopaminergic neurodegeneration assay. As previously described [ 23 ], synchronized late L3 larva worms (the BZ555 strain) were transferred to buffer containing 50 mM of 6-OHDA and 10 mM of ascorbic acid, incubated for one hour at 20°C, and mixed gently every 10 min. After one hour, worms were washed three times with M9 buffer and then incubated with or without SDG for 72 hours at 20°C.…”

Section: Methodsmentioning

confidence: 99%

Secoisolariciresinol Diglucoside Delays the Progression of Aging-Related Diseases and Extends the Lifespan ofCaenorhabditis elegansvia DAF-16 and HSF-1

Tan

Zhou

et al. 2020

Oxidative Medicine and Cellular Longevity

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Secoisolariciresinol diglucoside (SDG) is a phytoestrogen and rich in food flaxseed, sunflower seeds, and sesame seeds. Among the beneficial pharmacological activities of SDG on health, many are age related, such as anticancer, antidiabetes, antioxidant, and neuroprotective effects. Thus, we investigated if SDG had an effect on antiaging in Caenorhabditis elegans (C. elegans). Our results showed that SDG could extend the lifespan of C. elegans by up to 22.0%, delay age-related decline of body movement, reduce the lethality of heat and oxidative stress, alleviate dopamine neurodegeneration induced by 6-hydroxydopamine (6-OHDA), and decrease the toxicity of Aβ protein in C. elegans. SDG could increase the expression of the downstream genes of DAF-16, DAF-12, NHR-80, and HSF-1 at mRNA level. SDG could not extend the lifespan of mutants from genes daf-16, hsf-1, nhr-80, daf-12, glp-1, eat-2, and aak-2. The above results suggested that SDG might enhance the stress resistance, delay the progression of aging-related diseases, and extend the lifespan of C. elegans via DAF-16 and HSF-1.

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“…They have been reported to exhibit numerous beneficial properties including antiapoptotic, antioxidant, anti-inflammatory, and antifibrotic as well as gastro- and hepatoprotective effects. Studies have revealed the possible application of β -amyrin in Parkinson's disease therapy [ 21 , 22 ]. The examples of amyrins found in bee propolis are presented in Figure 3 .…”

Section: Introductionmentioning

confidence: 99%

Antioxidant Potential of Propolis, Bee Pollen, and Royal Jelly: Possible Medical Application

Kocot

Kiełczykowska

Luchowska-Kocot

et al. 2018

Oxidative Medicine and Cellular Longevity

365

358

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Honeybees products comprise of numerous substances, including propolis, bee pollen, and royal jelly, which have long been known for their medicinal and health-promoting properties. Their wide biological effects have been known and used since antiquity. Bee products are considered to be a potential source of natural antioxidants such as flavonoids, phenolic acids, or terpenoids. Nowadays, the still growing concern in natural substances capable of counteracting the effects of oxidative stress underlying the pathogenesis of numerous diseases, such as neurodegenerative disorders, cancer, diabetes, and atherosclerosis, as well as negative effects of different harmful factors and drugs, is being observed. Having regarded the importance of acquiring drugs from natural sources, this review is aimed at updating the current state of knowledge of antioxidant capacity of selected bee products, namely, propolis, bee pollen, and royal jelly, and of their potential antioxidant-related therapeutic applications. Moreover, the particular attention has been attributed to the understanding of the mechanisms underlying antioxidant properties of bee products. The influence of bee species, plant origin, geographic location, and seasonality as well as type of extraction solutions on the composition of bee products extracts were also discussed.

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Anti-Parkinsonian effects of β-amyrin are regulated via LGG-1 involved autophagy pathway in Caenorhabditis elegans

Cited by 44 publications

References 73 publications

Maackiain Ameliorates 6-Hydroxydopamine and SNCA Pathologies by Modulating the PINK1/Parkin Pathway in Models of Parkinson’s Disease in Caenorhabditis elegans and the SH-SY5Y Cell Line

Maackiain Ameliorates 6-Hydroxydopamine and SNCA Pathologies by Modulating the PINK1/Parkin Pathway in Models of Parkinson’s Disease in Caenorhabditis elegans and the SH-SY5Y Cell Line

Secoisolariciresinol Diglucoside Delays the Progression of Aging-Related Diseases and Extends the Lifespan ofCaenorhabditis elegansvia DAF-16 and HSF-1

Antioxidant Potential of Propolis, Bee Pollen, and Royal Jelly: Possible Medical Application

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