Anti‐photoaging capability of antioxidant extract from <i><scp>C</scp>amellia japonica</i> leaf

Mizutani, Taeko; Hashimoto, Masaki

doi:10.1111/exd.12395

Cited by 24 publications

(22 citation statements)

References 16 publications

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“…This activity may be attributed to its high content of quercetin, quercetin-3-O-glucoside, quercitrin, and kaempferol. 4,12 Camellia japonica extracts exert their antioxidant activity by scavenging ROS and enhancing the activity of antioxidant enzymes, such as superoxide dismutase, catalase, and glutathione peroxidase. During the in vitro study, the antioxidant activity of the CJ extract was proven using the Western blot analysis, which showed increased expression of the PRX family and MnSOD in a dose-dependent manner.…”

Section: Discussionmentioning

confidence: 99%

Anti-Inflammatory and Antioxidative Effects of Camellia japonica on Human Corneal Epithelial Cells and Experimental Dry Eye: In Vivo and In Vitro Study

Lee

Choi

Cui

et al. 2017

Invest. Ophthalmol. Vis. Sci.

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Citation: Lee HS, Choi J-H, Cui L, et al. Anti-inflammatory and antioxidative effects of Camellia japonica on human corneal epithelial cells and experimental dry eye: in vivo and in vitro study. Invest Ophthalmol Vis Sci. 2017;58:119658: -120758: . DOI:10.1167 PURPOSE. To analyze the anti-inflammatory and antioxidative effects of Camellia japonica (CJ) on human corneal epithelial (HCE) cells and its therapeutic effects in a mouse model of experimental dry eye (EDE). METHODS.Camellia japonica extracts of varying concentrations (0.001%, 0.01%, and 0.1%) were used to treat HCE cells. Dichlorofluorescein diacetate (DCF-DA) and dihydroethidium (DHE) assays were performed. The production of peroxiredoxin (PRX) 1-6 and manganesedependent superoxide dismutase (MnSOD) in HCE cells was assessed using Western blot analysis. Furthermore, eye drops containing 0.001%, 0.01%, or 0.1% CJ extract or a balanced salt solution (BSS) were applied to the EDE. Clinical parameters were measured 7 days after treatment. The levels of inflammatory markers and intracellular reactive oxygen species (ROS) were measured.RESULTS. Treatment with 0.01% and 0.1% CJ extracts decreased apoptosis in HCE cells. In addition, band intensities of PRX 1, 4, and 5, as well as MnSOD, after hydrogen peroxide (H 2 O 2 ) treatment showed a significant improvement after pretreatment with 0.01% and 0.1% CJ extracts. Mice treated with 0.1% CJ extract showed significantly improved clinical parameters when compared to those of the EDE control and BSS groups. A significant decrease in the levels of inflammatory markers and intracellular ROS was observed in the 0.01% and 0.1% CJ extract groups.CONCLUSIONS. Camellia japonica extracts promoted antioxidative protein expression and suppressed apoptosis in HCE cells. Furthermore, CJ extracts improved clinical signs of dry eye and reduced oxidative stress and the expression of inflammatory markers, suggesting that eye drops containing CJ extract could be used as an adjunctive treatment for dry eye.

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Section: Discussionmentioning

confidence: 99%

Anti-Inflammatory and Antioxidative Effects of Camellia japonica on Human Corneal Epithelial Cells and Experimental Dry Eye: In Vivo and In Vitro Study

Lee

Choi

Cui

et al. 2017

Invest. Ophthalmol. Vis. Sci.

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“…Although the human immune system has protective molecules involved in absorbing harmful UV light and scavenging ROS, including pigments and proteins, these molecules cannot heal the accumulated damage caused by prolonged and chronic UVB exposure . Accordingly, it is necessary to develop reagents and antioxidants capable of blocking UVB‐induced photoageing, especially those with molecular mechanisms of action . Therefore, to identify the molecular pathway associated with UVB‐induced photoageing, we focused on Hh signalling based on the high correlation of photoageing with skin cancer and crucial roles of Hh signalling in skin tumorigenesis.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of hedgehog signalling attenuates UVB‐induced skin photoageing

Kim

Yang

et al. 2015

Experimental Dermatology

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The hedgehog (Hh) signalling pathway regulates normal development and cell proliferation in metazoan organisms, but its aberrant activation can promote tumorigenesis and progression of a variety of aggressive human cancers including skin cancer. Despite its importance, little is known about its role in photoageing, a type of UV-induced skin lesions. In this study, we investigated the involvement of Hh signalling in the photoageing process as well as the use of an Hh-regulating alkaloid compound as a novel therapeutic drug to regulate photoageing in keratinocytes. We found that UVB induced Hh signalling by the expression of Hh ligands and Hh-mediated transcription factors, respectively. Moreover, UVB-induced Hh activation relied on mitogen-activated protein kinase (p38, ERK and JNK) activity and inflammatory responses (upregulation of COX-2, IL-1β, IL-6 and TNF-α), resulting in premature senescence and photoageing in vitro and in vivo. Notably, a selected Hh inhibitor, evodiamine, mediated photoageing blockade in a mouse skin model. Taken together, our findings demonstrated that Hh signalling is associated with UVB-induced photoageing, while pharmacological inhibition of Hh signalling significantly reduced experimental photoageing, indicating its potential for use as a therapeutic target for this disease.

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“…HDF cells were washed with PBS, covered with PBS and exposed to different UVB radiation doses (50-3000 mJ/cm 2 ) or exposure times (one or three times), as described previously (Mizutani & Masaki 2014;Xu et al 2014). The distance from the lamp to the culture plate was 5 cm.…”

Section: Uvb Exposure Of Hdfmentioning

confidence: 99%

Inhibitory effects of Schisandra chinensis extract on acne-related inflammation and UVB-induced photoageing

Guo

Yang

et al. 2016

Pharmaceutical Biology

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Context: Schisandra chinensis (Turcz.) Baill. (Schisandraceae) fruit extract (SFE) has been reported to induce non-specific tissue protection against inflammation in vivo. However, the effects of SFE on Propionibacterium acnes-stimulated acne and UVB-irradiated photoageing have yet to be investigated. Objective: To systematically investigate the effects of SFE against P. acnes and photoageing in vitro. Materials and methods: Qualitative and quantitative analyses of SFE were performed by HPLC. SFE concentrations from 2.5 to 50 lg/mL were tested. Specifically, ELISA was used to examine the levels of proinflammatory cytokines in THP-1 cells as well as of collagen I and matrix metalloproteinases-1 in HDF cells. The anti-bacterial effect of SFE was determined using the microdilution broth method. Glutathione and malondialdehyde levels were examined using the colorimetric and TBA methods, respectively. The degree of ageing was determined by cytochemical staining. Results: SFE significantly inhibited P. acnes growth (MIC 0.5 mg/mL) and 50 lg/mL of SFE suppressed the production of interleukin-1b, interleukin-8 and tumour necrosis factor a, by 59.67%, 62.69% and 68.30%, respectively, in P. acnes-stimulated THP-1 cells. Additionally, 10 lg/mL of SFE suppressed photoageing in UVB-exposed fibroblasts by decreasing metalloproteinase levels by 88.4%, inducing collagen by 58.4% and activating the anti-oxidant defence system, by limiting lipid peroxidation by 51.1% and increasing glutathione production by 34.1% (2.5 lg/mL SFE). Discussion and conclusion: These results indicated that SFE could significantly ameliorate the inflammatory state in P. acnes-stimulated THP-1 and UVB-irradiated HDF cells, suggesting its potential as a novel agent for acne therapy and photoageing prevention. ARTICLE HISTORY

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Anti‐photoaging capability of antioxidant extract from Camellia japonica leaf

Cited by 24 publications

References 16 publications

Anti-Inflammatory and Antioxidative Effects of Camellia japonica on Human Corneal Epithelial Cells and Experimental Dry Eye: In Vivo and In Vitro Study

Anti-Inflammatory and Antioxidative Effects of Camellia japonica on Human Corneal Epithelial Cells and Experimental Dry Eye: In Vivo and In Vitro Study

Inhibition of hedgehog signalling attenuates UVB‐induced skin photoageing

Inhibitory effects of Schisandra chinensis extract on acne-related inflammation and UVB-induced photoageing

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