Anti-photoaging effects of flexible nanoliposomes encapsulated Moringa oleifera Lam. isothiocyanate in UVB-induced cell damage in HaCaT cells

Abstract

Skin photoaging is premature skin aging damage that occurs after repeated exposure to ultraviolet (UV) radiation. Although isothiocyanates extracted from the moringa tree ( Moringa oleifera Lam. ) (MITC) exhibit excellent effects against skin photoaging, its application is restricted because of its characteristics, such as extremely low water solubility, bioavailability, and easy degradation. Currently, flexible nanoliposomes have gained increasing interest as a biocompatible polymer for applications such as transdermal drug delivery. We prepare amphiphilic hyaluronic acid (HA) conjugated with ceramide (CE) to modify nanoliposomes for MITC (HACE/MITC NPs) delivery. The HACE/MITC nanoparticles (NPs) are prepared and characterized for entrapment efficiency, particle size, polydispersity index, zeta potential, in vitro release, in vivo skin permeation, and in vitro protective effect of photoaging. The zeta potential of MITC NPs and HACE/MITC NPs is −24.46 mV and −24.93 mV, respectively. After modification of HACE, the entrapment efficient of MITC liposome increased from 62.54% to 70.67%, and the particle size decreased from 266.1 nm to 192.8 nm. In vivo skin permeation, permeated drug increased from 49.42 to 71.40%. Moreover, the results showed that the entrapment of MITC in nanoliposomes improves its stability, efficacy, and skin permeation. Further, HACE/MITC NPs are favorable for uptake by HaCaT cells without requiring changes in cell morphology, which significantly improves the activities of antioxidant enzymes, scavenges UVB-induced reactive oxygen species, protects skin from damage, and reduces MMP-1, MMP-3, and MMP-9 expression caused by radiation-induced photoaging. Our results strongly suggest that flexible nanoliposomes successfully improved the cell membrane permeation of MITC, and that anti-photoaging and HACE/MITC NPs can potentially be used as candidates for photoaging therapy.

A Comprehensive Review of Moringa oleifera Bioactive Compounds—Cytotoxicity Evaluation and Their Encapsulation

“…By using this method, unstable liquid compounds can be converted into free-flowing stable powder. Core bioactive components have been encapsulated using a variety of techniques, including cross-linking gelation, freeze-drying, microemulsion, spray drying, and different nanoformulations (nanoliposomes) ( Figure 3 ) [ 72 , 73 , 74 ].…”

Cyclodextrin-Coordinated Liposome-in-Gel for Transcutaneous Quercetin Delivery for Psoriasis Treatment

“…To investigate the permeation-enhancing mechanism of the HPCD-Lipo formulation, we analyzed the treated skin by FTIR and Raman analyses. Generally, three regions with related to barrier function are often investigated in the transdermal study, including (1) IR absorbance at 2920 and 2850 cm –1 representing asymmetric vibration (ν as CH 2 ) and symmetric stretching vibration (ν s CH 2 ) of the methylene groups of intercellular lipids, (2) vibrations of amide I (1640 cm –1 ) and amide II (1550 cm –1 ) bands related to the SC keratin that is distinct from the ceramide amide bond in the skin (1612 cm –1 ), and (3) the peaks ranging 1060–1130 cm –1 related to the fluidity of skin lipids. − The HPCD/QT Lipo@gel treatment resulted in a red shift of both ν as CH 2 (from 2916 to 2925 cm –1 ) and ν s CH 2 (from 2849 to 2852 cm –1 ) (Figure B). The red shift of ν as CH 2 and ν s CH 2 indicated that the orderly arrangement of the skin matrical lipids was compromised .…”

Moringa oleiferaLam.: a comprehensive review on active components, health benefits and application

“…13–15 Furthermore, the isothiocyanates contained in M. oleifera seeds also play a crucial role in anti-inflammatory, antioxidant, antibacterial and anticancer. 16 And the essential oil has coagulant effect that can accelerate wound healing. 17…”