Anti-platelet and Anti-thrombotic Effects of a Poly-ingredient formulation: In vitro and in vivo experimental evidences

Viswanatha, Gollapalle Lakshminarayanashastry; Rafiq, Mohamed; Rajesh, Sarvi Yadav; Ks, Sandeep Rao; Azeemuddin, Mohammed; Anturlikar, S. D.; Rangesh, Paramesh; Ps, Patki

doi:10.5001/omj.2012.127

Cited by 3 publications

(4 citation statements)

References 19 publications

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“…This is illustrated by the increased thrombus burden seen in this study using the in vivo IVC ligation model. 45,46 Future studies in proteinuric rats using a variety of thrombus-inducing techniques may help to elucidate which hemostatic components are most critical to nephrosis-associated thrombus development. 47,48 Although the global hemostasis assays used in this study are advantageous for identifying the summative hemostatic effect of a complex coagulopathy, such as NS, they do not directly assess derangements in the subcomponents of the hemostatic system that have been described in human NS.…”

Section: Discussionmentioning

confidence: 99%

“…45,46 After urine collection on day 9, the rats were deeply anesthetized by isoflurane inhalation (3%-5%), and IVC ligation was performed. After a midline laparotomy to expose the IVC at the level of the renal veins by careful blunt dissection, a 6-0 prolene suture was looped around the IVC immediately caudal to the origin of the renal veins and ligated to completely occlude blood flow.…”

Section: Ivc Ligation Experimentsmentioning

confidence: 99%

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Disease Severity Correlates with Thrombotic Capacity in Experimental Nephrotic Syndrome

Kerlin

Waller

Sharma

et al. 2015

Journal of the American Society of Nephrology

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Thrombotic disease, a major life-threatening complication of nephrotic syndrome, has been associated with proteinuria and hypoalbuminemia severity. However, it is not fully understood how disease severity correlates with severity of the acquired hypercoagulopathy of nephrotic syndrome. Without this knowledge, the utility of proteinuria and/or hypoalbuminemia as biomarkers of thrombotic risk remains limited. Here, we show that two well established ex vivo hypercoagulopathy assays, thrombin generation and rotational thromboelastometry, are highly correlated with proteinuria and hypoalbuminemia in the puromycin aminonucleoside and adriamycin rat models of nephrotic syndrome. Notably, in the puromycin aminonucleoside model, hyperfibrinogenemia and antithrombin deficiency were also correlated with proteinuria severity, consistent with reports in human nephrotic syndrome. Importantly, although coagulation was not spontaneously activated in vivo with increasing proteinuria, vascular injury induced a more robust thrombotic response in nephrotic animals. In conclusion, hypercoagulopathy is highly correlated with nephrotic disease severity, but overt thrombosis may require an initiating insult, such as vascular injury. Our results suggest that proteinuria and/or hypoalbuminemia could be developed as clinically meaningful surrogate biomarkers of hypercoagulopathy to identify patients with nephrotic syndrome at highest risk for thrombotic disease and potentially target them for anticoagulant pharmacoprophylaxis.

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Section: Discussionmentioning

confidence: 99%

Section: Ivc Ligation Experimentsmentioning

confidence: 99%

Disease Severity Correlates with Thrombotic Capacity in Experimental Nephrotic Syndrome

Kerlin

Waller

Sharma

et al. 2015

Journal of the American Society of Nephrology

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“…One study reported that aspirin 100µM showed 85.3% and 87.8% inhibition against adenosine 5'-diphosphate (ADP) and adrenalineinduced platelet aggregation, respectively. 19 However, our patient also had several risk factors for ischemic heart disease and we cannot rule out definitively other sources of embolism. It is possible that the increased risk of MI in patients on dabigatran is an associative effect rather than a causative one.…”

Section: Discussionmentioning

confidence: 97%

Acute Myocardial Infarction after Switching from Warfarin to Dabigatran

Abuzeid¹,

Al-Lawati²,

Fam³

2015

Oman Med J

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Dabigatran etexilate is a recently approved direct thrombin inhibitor (DTI), which is superior to warfarin in the prevention of stroke and systemic embolism in patients with atrial fibrillation (AF). However, dabigatran use is associated with an increased risk of myocardial infarction (MI) compared to warfarin. The mechanisms for this association effect remain speculative. We present a case of an acute MI and cardiac arrest in a patient with chronic AF who had been recently switched from warfarin to dabigatran. Urgent coronary angiography, at St. Michael's hospital (Toronto, Canada), revealed evidence of thromboembolism to the distal posterior descending artery. The patient was treated medically and switched back from dabigatran to warfarin. He did well and was discharged after an uneventful stay in the coronary care unit.

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“…Deep vein thrombosis (DVT) refers to the formation of one or more blood clots in one of the major blood vessels of the body, most commonly in the lower limbs. The most serious complication that can arise from DVT is a pulmonary embolism (PE), which occurs in more than one-third of patients with DVT and often causes sudden death [3][4][5]. Acetylsalicylic acid (ASA), commonly known as aspirin, is often used as a platelet aggregation inhibitor agent.…”

Section: Introductionmentioning

confidence: 99%

Antithrombotic Activity of Tamarindus Indica L. In Mice

2018

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Objective: This study aimed to investigate the antithrombotic activity of Tamarindus indica L. extract (TIE) in mouse models (in vivo).Methods: TIE was orally administered to mice at three different doses for 7 days. TIE-treated mice were used in two experiments of antithromboticactivity: An examination of bleeding time following tail cutting and an examination of survival rate after collagen-epinephrine-inducedthromboembolism. The TIE groups were observed after 7 days of treatment and compared to an aspirin-treated group and a control group.Results: Treatment with TIE led to a significant increase in bleeding time compared with that in the control group. TIE treatment also protected micefrom thromboembolic death, significantly increasing survival rates in a dose-dependent manner.Conclusion: TIE has the potential as an antithrombotic agent against platelet thromboembolism.

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Anti-platelet and Anti-thrombotic Effects of a Poly-ingredient formulation: In vitro and in vivo experimental evidences

Abstract: These findings suggest that Abana, a polyherbal formulation possesses anti-platelet and anti-thrombotic activities in the experimental models of platelet aggregation and deep venous thrombosis in rats.

Cited by 3 publications

References 19 publications

Disease Severity Correlates with Thrombotic Capacity in Experimental Nephrotic Syndrome

Disease Severity Correlates with Thrombotic Capacity in Experimental Nephrotic Syndrome

Acute Myocardial Infarction after Switching from Warfarin to Dabigatran

Antithrombotic Activity of Tamarindus Indica L. In Mice

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