Anti-Platelet Fraction Isolated from Galega Officinalis

Abstract

Summary.A fraction from crude extract of Galega offi cinalis has been purifi ed by column chromatography on Sephadex G-25, Sepharose 4B, DEAE-Cellulose and Sephadex G-100. The fi nal purifi cation factor of the fraction is 120. The peak in elution profi le after Sephadex G-150 shows a molecular weight of 100-140 kDa. The isolated fraction appears to have 74% polysaccharides and 23% of proteins. No loss of activity of the fi nal fraction is observed after storage for several months at 4 ºC and in lyophilized condition. The fraction compounds inhibit platelet aggregation induced by ADP, collagen and thrombin.