Anti-proliferative Activities of Solvent Fractions of Lees Extracts in Human Colorectal HCT116 Cells

Abstract

In the present study, we prepared eighty-five different kinds of lees extracts and their solvent fractions and investigated their anti-proliferative activities against human colorectal cancer HCT116 cells. HCT116 cells were treated with eighty-five solvent fractions of lees extracts and then cell viability was measured using MTS assay. Among the treated solvent fractions, three solvent fractions (KSD-E1-3, KSD-E2-3, and KSD-E4-3) were selected based on cell viability assay. In addition, we performed an oligo DNA microarray analysis to analyze the gene expression changes by treatment of KSD-E1-3 in HCT116 cells. Among the up-regulated genes, we selected 4 genes (NAG-1, ATF3, p21, and DDIT3) and performed RT-PCR using gene-specific primers. Among the treated solvent fractions, KSD-E1-3 dramatically induced the expressions of the four selected genes. In addition, we investigated whether the up-regulations of those genes were dependent on the transcription factor p53's presence using p53 null HCT116 cells. The results indicate that the up-regulations of NAG-1, ATF3, and DDIT3 are not dependent on the p53 presence, whereas p21 is dependent on the p53 presence. These findings may help to understand the molecular mechanisms of the anti-proliferative activity mediated by rice wine lees in human colorectal cancer cells.

Anti-proliferative and Pro-apoptotic Effects by Lees Extracts of Ehwa Makgeolli Containing Oriental Herbs

“…본 연구그룹은 선행연구에서 8종의 주박과 3종의 누룩으로 부터 총 85종의 추출물 및 분획물을 제조하고, 이들의 암세포 항 성장 활성을 보고한 바 있다 [7]. 본 연구에서는 Table 1에 나타낸 바와 같이 1차 선별된 주박 추출물 중 한방이화주 주박 …”

Application of the Lees of Domestic Traditional Wine and its Useful Biological Activity

“…1A). 한편, 기존의 보고된 주박의 생리활성으로는 항산화 활성 [14,17,20,21,27]이 가장 많으며, 최근에는 항혈전 [14,15,16,17], 항염증 [7,30,31,34,38], 항균 [14,15,20] 및 암세포 성장억제 활성 [7,8,38]들이 보고되고 있다. 그 외, 항주름 및 미백활성 [26,37], 항당뇨 [10,18], 알러지 감소 [9], 항비만 및 고혈압 억제 활성 [22,25,34]도 알려져 있어(Fig.…”