Anti‐proliferative activity of bovine blood hydrolysates towards cancer cells in culture

Abstract

Four proteins, α/β globulin, serum albumin, γ‐globulin and fibrinogen, were isolated from bovine blood and hydrolysed using papain. Hydrolysates were assessed for non‐cellular and cellular antioxidant activity. The anti‐proliferative activity of hydrolysed fractions was assessed in a number of cancer cell lines including U937 lymphoma cells, MCF‐7 breast cancer cells, HepG2 hepatocytes and Caco‐2 epithelial colorectal adenocarcinoma cells. Anti‐inflammatory activity of the hydrolysates was also assessed. Hydrolysates generated from γ‐globulin or fibrinogen had significant antioxidant activity in non‐cellular assays. Hydrolysates were also found to be highly toxic to different cancer cell lines, in particular U937 lymphoma cells when assessed using the MTT assay. The fibrinogen hydrolysate was the most toxic sample and toxicity appeared to correlate with its non‐cellular antioxidant activity. None of the hydrolysates had significant anti‐inflammatory activity. The high cytotoxicity of the γ‐globulin and the fibrinogen hydrolysates towards cancer cells may indicate a potential use as anti‐proliferative agents.

Bioactivity of bovine lung hydrolysates prepared using papain, pepsin, and Alcalase

“…Following on from these studies, oxidative DNA damage was induced in cells using H 2 O 2 at a concentration of 80 μmol L −1 for 30 min. Previously published studies using the comet assay have employed similar concentrations of H 2 O 2 with U937 cells (Crowley et al, ; O'Sullivan, Lafarga, Hayes, & O'Brien, ).…”

Bioactive peptides derived from bovine and porcine co-products: A review

“…In addition, several studies have demonstrated the potential of blood proteins for use as technofunctional ingredients and as novel sources of bioactive peptides. For example, O'Sullivan, Lafarga, Hayes and O'Brien () recently suggested the potential of papain (EC 3.4.22.2) hydrolysates of bovine blood proteins as antiproliferative agents using a number of cancer cell lines including U937 lymphoma cells, MCF‐7 breast cancer cells, HepG2 hepatocytes, and Caco‐2 epithelial colorectal adenocarcinoma cells.…”

“…In this study, no significant effects on the antiproliferative properties of the extracts were observed after a simulated gastrointestinal digestion. In addition, Castro, Maria, Bouhallab, and Sgarbieri () suggested the antitumoral potential of an enzymatic hydrolysate of porcine collagen on murine hepatoma cells and O'Sullivan et al () demonstrated the potential of papain hydrolysates of bovine blood proteins as antiproliferative agents using a number of cancer cell lines.…”

Contribution of different molecular weight fractions to anticancer effect of sweet potato protein hydrolysates by six proteases on HT‐29 colon cancer cells

“…Protein hydrolysates from food have shown multiple bioactivities, for example anticancer, antioxidant, mineral binding, antimicrobial, antihypertensive and immunomodulatory effects (Girón‐Calle et al ., ; García Fillería & Tironi, ; Sae‐leaw et al ., ; Li‐Chan, ; O'Sullivan et al ., ). Numerous protein hydrolysates and peptides with anticancer effects on colon cancer cells have been obtained from chickpea (Girón‐Calle et al ., ), soya bean (Rayaprolu et al ., ) and rice (Kannan et al ., ).…”