Anti-proliferative and apoptosis-inducing activities of juglone in LS-174T cells

DNA hypomethylation concomitant with the overproduction of ROS induced by naphthoquinone juglone on tobacco BY-2 suspension cells

“…Similar results have been reported by Babula et al (2009a) who presented an ability of naphthoquinones to inhibit growth of plant cells. However, cytotoxic effects of naphthoquinones have mostly been studied in animal cell lines Xu et al, 2012;Li et al, 2013), cells different from the BY-2 cells, which represent a plant cell model. The cytotoxic effect of naphthoquinones is mediated by their ability to produce ROS.…”

Phytotoxic action of naphthoquinone juglone demonstrated on lettuce seedling roots

“…This fact indicates unambiguous role of ROS in the juglone-induced apoptosis. There are many authors who investigated ability of juglone to induce apoptosis in various (tumour) cell models, such as human prostate cancer LNCaP cells (Xu et al, 2013), LS-174T cells (Li et al, 2013), human leukemia cell HL-60 (Xu et al, 2012), or human gastric cancer SGC-7901 cells (Ji et al, 2011). To detect changes in mitochondrial membrane potential, we used JC-1 probe that shows dual emission (based on the creation of aggregates on monomers in dependence on mitochondrial membrane potential).…”

Preparation and Antiproliferative Activity Evaluation of Juglone-Loaded BSA Nanoparticles

“… 7 JUG is also a potent cytotoxic compound in vitro on various human tumor cell lines. 8 - 16 Nevertheless, JUG has cytotoxic effects on normal healthy cells by different mechanisms. 17 - 20 The side effects, as well as low water solubility, restrict the application of JUG as an antitumor agent rationalizing the studies to rectify these problems.…”