Anti-proliferative and Apoptotic Effects of morin in human Leukemia cell lines (HUT-78)

Abstract

Cancer is one of the main cause of mortality in the world which appears by the effect of enviromental physico-chemical mutagen and carcinogen agents. Lymphoblastic leukemia is one the prevalence cancer in human. Chemotherapy and radiotherapy improve remission of the disease but some probability relaps is observed between 20-30% patients. Tumorgenesis and tumor progression are strongly associated with abnormal apoptosis. A number of natural antitumor drugs exert their therapeutic effect by inducing or promoting apoptosis [1]. In the last years, many studies have been performed on the anticancer effects of flavonoids. In cancer therapies, natural compounds have been considered as effective inhibitor agents. There is evidence to support the concept that 2`,3,4`,5,7 pentha hydroxyl flavone (morin) has a great potential to develop into novel cancer preventative agent [2]. Aim To investigate the effect of morin on apoptosis of human leukemia cells (Nalm-6 and HUT-78). Material and methods In this study human chronic lymphocytic leukemia (HUT-78) and human leukemia pre B-cells (Nalm-6) were cultured in RPMI 1640[Sigma], supplemented with 10% fetal bovine serum (FBS), penecilinstreptomycin and L-glutamine. The cultures were incubated at 37ºC, 5% CO2 and then inhibitory effect of morin on their proliferation was measured by MTT assay. Cell cycle progression monitored by Sub-G1 apoptosis assay using flow cytometry and Hoechst staining method.

Anti-Mutagenicity and Apoptotic Effects of Teucrium polium L. Essential Oil in HT29 Cell Line

“…To determine the viability of cells under different conditions, the microculture tetrazolium technique (MTT) assay was used (16,17). Since MTT is capable of reducing the MTT, it's based on the reducing MTT to purple Formazan by mitochondrial reductase in alive cells.…”

“…To analyze the cells for sub-G1 apoptosis, we followed the methodology used by Karimi et al (16). Flow cytometric analysis of PI-labeled cells was used to determine the cellular DNA content.…”

Dietary Intervention by Phytochemicals and Their Role in Modulating Coding and Non-Coding Genes in Cancer

“…Morin (3,2′,4′,5,7-pentahydroxyflavone) is a flavone that belongs to the Moraceae plants family and is recognized for its anti-carcinogenic and anti-inflammatory roles in different pathologies, including cancer [ 25 , 59 , 60 ]. Furthermore, it has been demonstrated to act as a chemopreventive agent in oral malignancies [ 25 , 59 ].…”