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Although we have not observed any significant correlation between the HES1 expression and notch activation in ESCC, this study is the first report that elucidated the HES1 underexpression in ESCC and revealed its correlation with the invasiveness of ESCC.

Cancer is one of the main cause of mortality in the world which appears by the effect of enviromental physico-chemical mutagen and carcinogen agents. Lymphoblastic leukemia is one the prevalence cancer in human. Chemotherapy and radiotherapy improve remission of the disease but some probability relaps is observed between 20-30% patients. Tumorgenesis and tumor progression are strongly associated with abnormal apoptosis. A number of natural antitumor drugs exert their therapeutic effect by inducing or promoting apoptosis [1]. In the last years, many studies have been performed on the anticancer effects of flavonoids. In cancer therapies, natural compounds have been considered as effective inhibitor agents. There is evidence to support the concept that 2`,3,4`,5,7 pentha hydroxyl flavone (morin) has a great potential to develop into novel cancer preventative agent [2]. Aim To investigate the effect of morin on apoptosis of human leukemia cells (Nalm-6 and HUT-78). Material and methods In this study human chronic lymphocytic leukemia (HUT-78) and human leukemia pre B-cells (Nalm-6) were cultured in RPMI 1640[Sigma], supplemented with 10% fetal bovine serum (FBS), penecilinstreptomycin and L-glutamine. The cultures were incubated at 37ºC, 5% CO2 and then inhibitory effect of morin on their proliferation was measured by MTT assay. Cell cycle progression monitored by Sub-G1 apoptosis assay using flow cytometry and Hoechst staining method.

Background:Acute promyelocytic leukemia (APL) is an acute leukemia diagnosed by translocation of chromosomes 15 and 17 [T (15,17)] and aggregation of neoplastic promyelocytes which are incapable of being converted into mature cells. Today, many tend to use medicinal herbs in studies and clinical applications for treatment of cancers. Cinnamon with scientific name “cinnamomumzelanicum” is a shrub of Laurales order, lauraceae family with cinnamomum genus. It is a medicinal shrub with anti-proliferation effect on tumor cells. This study was conducted to determine the effects of aqueous cinnamon extract on HL-60 cells as a model for APL.Materials and Methods:In this in vitro experimental study, HL-60 cell line was cultured under the influence of cinnamon extract's concentrations of 0.01, 0.1, 1, and 2 mg/ml in with intervals of 24, 48, and 72 h. Growth inhibition and toxic effects of cinnamon extract were evaluated through tetrazolium salt reduction. The effect of this herb on the cell cycle was studied by flow cytometry. The Hoechst stain was used to detect apoptotic cell nuclei.Results:Cinnamon extract inhibited the growth of HL-60 cells as correlated with concentration and time. After 72 h of treating HL-60 cells with 0.01 mg/l cinnamon extract, the growth of cells was inhibited by 90.1%. Cinnamon extract stopped the cell cycle in G1 phase and the Hoechst staining verified the apoptotic process in those cells.Conclusion:Considering the inhibitory property of cinnamon extract, we recommend it as a single drug or besides other medications for treating promyelocytic leukemia.

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