Anti-proliferative and cytotoxic effect of Iranian snake (Vipera raddei kurdistanica) venom on human breast cancer cells via reactive oxygen species-mediated apoptosis

Malekara, Ehsan; Pazhouhi, Mona; Rashidi, Iraj; Jalili, Cyrus

doi:10.4103/1735-5362.278717

Cited by 9 publications

(5 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The anti-proliferative properties of venoms extracted from vertebrates and invertebrate species have been widely reported, where the potential application of venoms as anti-cancer agents has received considerable attention during the past decade, especially with snake venoms (for reviews, see [37][38][39][40]). Anticancer activity in various snake venoms has been reported for pancreatic tumor cells [41], human breast cancer (MCF-7), human hepatocellular carcinoma (HepG2) and human prostate carcinoma (DU145) cell lines [42], human leukemic cell lines (U937 and K562) and sarcoma in a Balb C mice model [43], colorectal and breast cancer cell lines [44], human prostate adenocarcinoma (LNCaP), human breast cancer (MCF-7), human colon adenocarcinoma (HT-29) and human osteoblastic osteosarcoma (Saos-2) cells [45], and human breast carcinoma cells (MDA-MB-231 and MCF-7) [46,47].…”

Section: Venoms As a Source Of Potential Anti-cancer Agentsmentioning

confidence: 99%

Bioactive Properties of Venoms Isolated from Whiptail Stingrays and the Search for Molecular Mechanisms and Targets

Doupnik,

Luer,

Walsh

et al. 2024

Preprint

View full text Add to dashboard Cite

The venom-containing barb attached to their ‘whip-like’ tail provides stingrays a defensive mechanism for evading predators such as sharks. From human encounters, dermal stingray envenomation is characterized by intense pain often followed by tissue necrosis occurring over several days to several weeks. The bioactive components in stingray venoms (SRV’s) and their molecular targets and mechanisms that mediate these complex responses are not well understood. Given the utility of venom-derived proteins from other venomous species for biomedical and pharmaceutical applications, we set out to characterize the bioactivity of SRV from three local species that belong to the Dasyatoidea ‘whiptail’ superfamily. Multiple cell-based assays were used to quantify and compare the in vitro effects of these SRV’s on different cell lines. All three SRV’s demonstrated concentration-dependent growth inhibitory effects on three different human cell lines tested. In contrast, a mouse fibrosarcoma cell line was markedly resistant to all three SRV’s, indicating the molecular target(s) for mediating the SRV effects are not expressed on these cells. The multifunctional SRV responses were characterized by an acute disruption of cell adhesion leading to apoptosis. These findings aim to guide future investigations of identified SRV proteins and their molecular targets for potential use in biomedical applications.

show abstract

Section: Venoms As a Source Of Potential Anti-cancer Agentsmentioning

confidence: 99%

Bioactive Properties of Venoms Isolated from Whiptail Stingrays and the Search for Molecular Mechanisms and Targets

Doupnik,

Luer,

Walsh

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…The antiproliferative properties of venoms extracted from vertebrates and invertebrate species have been widely reported, and the potential application of venoms as anticancer agents has received considerable attention during the past decade, especially with snake venoms (for reviews, see [40][41][42][43]). Anticancer activity in various snake venoms has been reported for pancreatic tumor cells [44], human breast cancer (MCF-7), human hepatocellular carcinoma (HepG2), and human prostate carcinoma (DU145) cell lines [45], human leukemic cell lines (U937 and K562), and sarcoma in a Balb C mice model [46], colorectal and breast cancer cell lines [47], human prostate adenocarcinoma (LNCaP), human breast cancer (MCF-7), human colon adenocarcinoma (HT-29), human osteoblastic osteosarcoma (Saos-2) [48], and human breast carcinoma cells (MDA-MB-231 and MCF-7) [49,50].…”

Section: Venoms As a Source Of Potential Anticancer Agentsmentioning

confidence: 99%

Bioactive Properties of Venoms Isolated from Whiptail Stingrays and the Search for Molecular Mechanisms and Targets

Doupnik,

Luer,

Walsh

et al. 2024

Pharmaceuticals

View full text Add to dashboard Cite

The venom-containing barb attached to their ‘whip-like’ tail provides stingrays a defensive mechanism for evading predators such as sharks. From human encounters, dermal stingray envenomation is characterized by intense pain often followed by tissue necrosis occurring over several days to weeks. The bioactive components in stingray venoms (SRVs) and their molecular targets and mechanisms that mediate these complex responses are not well understood. Given the utility of venom-derived proteins from other venomous species for biomedical and pharmaceutical applications, we set out to characterize the bioactivity of SRV extracts from three local species that belong to the Dasyatoidea ‘whiptail’ superfamily. Multiple cell-based assays were used to quantify and compare the in vitro effects of these SRVs on different cell lines. All three SRVs demonstrated concentration-dependent growth-inhibitory effects on three different human cell lines tested. In contrast, a mouse fibrosarcoma cell line was markedly resistant to all three SRVs, indicating the molecular target(s) for mediating the SRV effects are not expressed on these cells. The multifunctional SRV responses were characterized by an acute disruption of cell adhesion leading to apoptosis. These findings aim to guide future investigations of individual SRV proteins and their molecular targets for potential use in biomedical applications.

show abstract

“…Viperidae MDA-MB-231 and MCF-7 Induces cytotoxicity and apoptosis Malekara et al (2020) toxin showed anticancer activities in S-180 sarcoma bearing mice by inhibiting tumour growth (Ghazaryan et al, 2015). C. cerastes venom inhibited the growth of mouse mammary tumour virus-induced mammary tumour cell line (RIII/Sa-MT) (El-Refael & Sarkar, 2009) or WEV + NP decreased activation of Bcl but increased that of caspase-3 of these breast cancer cell lines.…”

Section: Vipera Raddei Kurdistanicamentioning

confidence: 99%

“…Venom of the Iranian snake ( Vipera raddei kurdestanica ) induced significant cytotoxicity and ROS‐mediated apoptosis in human breast cancer cells (MCF‐7 and MDA‐MB‐231) in comparison to the normal human mammary epithelial cell line (MCF‐10a) in a dose‐ and time‐dependent manner (Malekara et al, 2020). In comparison to all tested cell lines, MCF‐7 cells were the most sensitive to snake venom as they had the lowest IC 50 values.…”

Section: Crude Snake Venom Anticancer Activitiesmentioning

confidence: 99%

“…In comparison to all tested cell lines, MCF‐7 cells were the most sensitive to snake venom as they had the lowest IC 50 values. The venom‐induced apoptosis was linked through the mitochondrial pathway, reduced mitochondrial membrane potential, the release of cytochrome C from the mitochondria, increased BAX/BCL‐2 family and alteration of the BCL‐2 protein families (Malekara et al, 2020).…”

Section: Crude Snake Venom Anticancer Activitiesmentioning

confidence: 99%

See 1 more Smart Citation

Snake venom toxins: Potential anticancer therapeutics

Offor,

Piater

2023

J of Applied Toxicology

View full text Add to dashboard Cite

Snake venom contains a cocktail of compounds dominated by proteins and peptides, which make up the toxin. The toxin components of snake venom attack several targets in the human body including the neuromuscular system, kidney and blood coagulation system and cause pathologies. As such, the venom toxins can be managed and used for the treatment of these diseases. In this regard, Captopril used in the treatment of cardiovascular diseases was the first animal venom toxin‐based drug approved by the US Food and Drug Administration and the European Medicines Agency. Cancers cause morbidity and mortality worldwide. Due to side effects associated with the current cancer treatments including chemotherapy, radiotherapy, immunotherapy, hormonal therapy and surgery, there is a need to improve the efficacy of current treatments and/or develop novel drugs from natural sources including animal toxin‐based drugs. There is a long history of earlier and ongoing studies implicating snake venom toxins as potential anticancer therapies. Here, we review the role of crude snake venoms and toxins including phospholipase A2, L‐amino acid oxidase, C‐type lectin and disintegrin as potential anticancer agents tested in cancer cell lines and animal tumour models in comparison to normal cell lines. Some of the anti‐tumour activities of snake venom toxins include induction of cytotoxicity, apoptosis, cell cycle arrest and inhibition of metastasis, angiogenesis and tumour growth. We thus propose the advancement of multidisciplinary approaches to more pre‐clinical and clinical studies for enhanced bioavailability and targeted delivery of snake venom toxin‐based anticancer drugs.

show abstract

Anti-proliferative and cytotoxic effect of Iranian snake (Vipera raddei kurdistanica) venom on human breast cancer cells via reactive oxygen species-mediated apoptosis

Cited by 9 publications

References 21 publications

Bioactive Properties of Venoms Isolated from Whiptail Stingrays and the Search for Molecular Mechanisms and Targets

Bioactive Properties of Venoms Isolated from Whiptail Stingrays and the Search for Molecular Mechanisms and Targets

Bioactive Properties of Venoms Isolated from Whiptail Stingrays and the Search for Molecular Mechanisms and Targets

Snake venom toxins: Potential anticancer therapeutics

Contact Info

Product

Resources

About