Anti-quenching NIR-II molecular fluorophores for in vivo high-contrast imaging and pH sensing

Wang, Shangfeng; Fan, Yong; Li, Dandan; Sun, Chia‐Chung; Lei, Zuhai; Lu, Lingfei; Wang, Ting; Zhang, Fan

doi:10.1038/s41467-019-09043-x

Cited by 417 publications

(394 citation statements)

References 46 publications

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“…Currently, there are mainly two methods for the designing of NIR‐II fluorophores: conjugation length enhancement and donor‐acceptor (D‐A) engineering . In the former case, increasing conjugation length can redshift both absorption and emission of the polymethine cyanines (such as IR‐26) to the NIR‐II region, whereas the fluorescence attenuates efficiently due to the solvatochromism‐caused quenching .…”

Section: Introductionmentioning

confidence: 99%

Constitutional Isomerization Enables Bright NIR‐II AIEgen for Brain‐Inflammation Imaging

Liu

Chen

et al. 2019

Adv Funct Materials

213

209

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The shortage of high quantum yield (QY) organic fluorophores in the second near‐infrared window (NIR‐II) has become a bottleneck in bioimaging field. Now, a simple strategy is proposed to address this: constitutional isomerization on the basis of the molecular design philosophy of aggregation‐induced emission. With the combination of backbone distortion and rotor twisting, the resultant NIR‐II fluorophore 2TT‐oC6B displays an emission peak at 1030 nm and a QY of 11% in nanoparticles, one of the highest reported so far. Control molecules confirm that the distorted backbone and twisted rotors play equally important roles in determining the fluorescence properties of the NIR‐II fluorophores. To allow for the targeting ability to reach deeply located diseases, neutrophils (NEs) are used to penetrate the brain tissues and accumulate in the inflammation site. Herein, it is shown that NEs carrying 2TT‐oC6B nanoparticles can penetrate the blood‐brain‐barrier and visualize the deeply located inflammation through an intact scalp and skull. Notably, the bright 2TT‐oC6B contributes to a significantly enhanced signal‐to‐background ratio of 30.6 in the brain inflammation site.

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Section: Introductionmentioning

confidence: 99%

Constitutional Isomerization Enables Bright NIR‐II AIEgen for Brain‐Inflammation Imaging

Liu

Chen

et al. 2019

Adv Funct Materials

213

209

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“…In contrast, small‐molecule‐based NIR‐II dyes offer distinct advantages with regard to potential clinical translation. Therefore, the development of organic small‐molecule fluorophores represents an important, newly emerging and dynamic research field in molecular imaging . In particular, organic fluorophores such as CH1055, which is based on a donor–acceptor–donor structure including a benzobisthiadiazole derivative as the acceptor, demonstrate attractive optical properties (NIR‐II emission spectra, good quantum yield, high photostability) and excellent in vivo performance .…”

Section: Introductionmentioning

confidence: 99%

An IR820 Dye–Protein Complex for Second Near‐Infrared Window and Photoacoustic Imaging

Qian

et al. 2019

Advanced Optical Materials

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Recently, much attention has been focused on the development of second near‐infrared window (NIR‐II, 1000–1700 nm) fluorescence imaging because of its reduced scattering, minimal absorption, and negligible autofluorescence. NIR‐II bioimaging allows the visualization of deep anatomical features with an unprecedented degree of clarity. In addition to the construction of a variety of new NIR‐II fluorophores, using a fluorescence tail emission greater than 1000 nm for conventional NIR‐I dyes represents a promising strategy for developing an NIR‐II imaging technique. Herein, the authors report tailoring a supramolecular assembly of human serum albumin (HSA) protein complexed with a sulfonated NIR‐I organic dye (IR820) to produce a brilliant 21‐fold increase in fluorescence for NIR‐II imaging. In vivo NIR‐II imaging with IR820–HSA allows noninvasive and dynamic visualization and monitoring of physiological and pathological conditions of the vascular system, lymphatic drainage system, and tumor‐bearing mice, as well as image‐guided tumor resection with high spatial and temporal resolution. Furthermore, photoacoustic imaging (PAI) of IR820–HSA in mouse tumor models also demonstrate good tumor accumulation. Overall, an IR820–protein complex with high biocompatibility can be easily constructed using a conventional NIR‐I dye that provides a new theranostic tool with high clinical translation potential.

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“…We proceeded to measure NIR‐II emission behavior of IR‐FD, QDs, and an IR‐FD/QDs mixture at >1100, 1100–1300, and >1500 nm windows, respectively, again confirming the nonoverlay of selected probes (Figure 1f). We used an 1% Intralipid solution to simulate tissue to test the penetration depth of the QDs compared with clinically used ICG probe . From the fluorescence images of capillaries filled with QDs or ICG solutions, we observed that the QDs achieved a better penetration depth at 9 mm with signal to background ratio (S/B) of ≈1.26, while ICG at 5 mm had S/B of ≈1.07, providing significant improvement of penetration and contrast with QDs in the NIR‐IIb window (Figure 1g).…”

mentioning

confidence: 97%

Multiplexed NIR‐II Probes for Lymph Node‐Invaded Cancer Detection and Imaging‐Guided Surgery

et al. 2020

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Tumor‐lymph node (LN) metastasis is the dominant prognostic factor for tumor staging and therapeutic decision‐making. However, concurrently visualizing metastasis and performing imaging‐guided lymph node surgery is challenging. Here, a multiplexed‐near‐infrared‐II (NIR‐II) in vivo imaging system using nonoverlapping NIR‐II probes with markedly suppressed photon scattering and zero‐autofluorescence is reported, which enables visualization of the metastatic tumor and the tumor metastatic proximal LNs resection. A bright and tumor‐seeking donor–acceptor–donor (D‐A‐D) dye, IR‐FD, is screened for primary/metastatic tumor imaging in the NIR‐IIa (1100–1300 nm) window. This optimized D‐A‐D dye exhibits greatly improved quantum yield of organic D‐A‐D fluorophores in aqueous solutions (≈6.0%) and good in vivo performance. Ultrabright PbS/CdS core/shell quantum dots (QDs) with dense polymer coating are used to visualize cancer‐invaded sentinel LNs in the NIR‐IIb (>1500 nm) window. Compared to clinically used indocyanine green, the QDs show superior brightness and photostability (no obvious bleaching even after continuous laser irradiation for 5 h); thus, only a picomolar dose is required for sentinel LNs detection. This combination of dual‐NIR‐II image‐guided surgery can be performed under bright light, adding to its convenience and appeal in clinical use.

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Anti-quenching NIR-II molecular fluorophores for in vivo high-contrast imaging and pH sensing

Cited by 417 publications

References 46 publications

Constitutional Isomerization Enables Bright NIR‐II AIEgen for Brain‐Inflammation Imaging

Constitutional Isomerization Enables Bright NIR‐II AIEgen for Brain‐Inflammation Imaging

An IR820 Dye–Protein Complex for Second Near‐Infrared Window and Photoacoustic Imaging

Multiplexed NIR‐II Probes for Lymph Node‐Invaded Cancer Detection and Imaging‐Guided Surgery

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