Anti-quorum Sensing Effetcs of Ethanolic Crude Extract ofAnethum graveolensL.

Abstract

In the recent years, effort to interfere with the quorum sensing (QS) has taken researchers consideration into the discovery and identification of bioactive compounds secreted by prokaryotes and eukaryotes. In this research, the anti-QS activity of thirty one plant species was detected through the inhibition of the QS related behaviors, violacein pigmentation in Chromobacterium violaceum CV026 and plant tissue maceration in Pectobacterium carotovorum. The results revealed that the arial parts extract of seven plant species interfered quorum sensing phenotypes in bacteria which amoung them, the dill (Anethum graveolens L.) extract effectively inhibited violacein production in CV026 and singificantly reduced tissue maceration in test plants. The result substantiates that the dill extract has decreased (>13 mm) violacein production in biosensor besides possessing slight antimicrobial property (<7.5 mm). Both potato tubers and calla-lily slices inoculated with Pcc-extract showed significant reduction in tissue maceration, while the tubers and slices inoculated with Pcc and Pcc-DMSO were strongly macerated. GC-MS analysis of the A. graveolens extract indicated that the oil was consisted eight phytocompounds of which the Eugenol (49.62 %) as major component. This results suggest that plants have mimic quorum-sensing signals which could be serving as potential sources to disrupt quorum sensing in associated bacteria.

The anti-quorum sensing activity and bioactive substance of a marine derived Streptomyces

“…The results showed that the bacterial growth was not affected in the lower concentration treatment (6.25 mg/mL), and slightly affected in the medium concentration treatment (12.5 mg/mL), which was similar to the control (25 mg/mL 3,4 dibromo-2(5H)-furanone) ( Figure 8). The violacein inhibitory activity of HY026 and compound F1-4 observed in this study was higher than or similar to some other natural substances reported in similar research [20,21], such as eugenol [22] and malyngolide [23]. Besides the remarkable violacein inhibitory activity, F1-4 and actinomycin D standard could also inhibit prodigiosin production in Serratia proteamaculans 657 with the inhibition zone of 13.5 and 14 mm at 25 mg/disc.…”

Quorum sensing inhibitory effect of hexanal on Autoinducer‐2 (AI‐2) and corresponding impacts on biofilm formation and enzyme activity in Erwinia carotovora and Pseudomonas fluorescens isolated from vegetables

“…There are few studies reported that EOs based on QS inhibitory (QSI) property which have developed as food preservatives to enhance food safety, including Murraya koenigii , Cuminium cymium , and Myristica fragrans (nutmeg) (Bai & Vittal, 2014). In addition, EO components such as carvacrol and thymol could regulate the biofilm formation in Pseudomonas fluorescens KM121 and cyclohexasiloxane, eugenol and 1,2‐benzenedicarboxylic acid could inhibit the virulence in Pectobacterium carotovorum (Makhfian et al., 2015; Myszka et al., 2016). However, the mechanism of QSI effect of these EOs and its components has not been studied in depth.…”