Anti-Quorum Sensing Potential of Antioxidant Quercetin and Resveratrol

Erdönmez, Demet; Rad, Abbas Yousefi; Aksöz, Nilüfer

doi:10.1590/1678-4324-2017160756

Cited by 17 publications

(15 citation statements)

References 26 publications

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“…Similar results were reported by He et al [19] that demonstrated the feasibility of attenuating the tolerance of P. aeruginosa to azithromycin by using marine oligosaccharides. Increased H 2 O 2 susceptibility was unexpected at rst, as quercetin is known as important antioxidants [11,12].…”

Section: Discussionmentioning

confidence: 99%

“…It is found commonly in many fruits, vegetables, leaves and grains [6]. Several investigations have reported the anti-microbial, anti-quorum sensing, anti-oxidant, anti-carcinogenic, anti-in ammatory, anti-apoptotic and anti-histamine effects of quercetin [4,6,11,12]. One strategy that can be useful in the treatment of MDR P. aeruginosa infections is the synergy using combinations of natural compounds with antibiotics leading to enhancement of antibacterial activity of antibiotics.…”

Section: Introductionmentioning

confidence: 99%

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Anti-quorum sensing activity of quercetin in combination with imipenem and ciprofloxacin against Pseudomonas aeruginosa PAO1

Taghavi

Zeighami

Heidari

et al. 2021

Preprint

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Background The inhibition of quorum sensing (QS) is considered as a novel strategy for development of antipathogenic agents and management of nosocomial infections caused by Pseudomonas aeruginosa. The present study aimed to investigate the anti-quorum sensing activity of sub-MIC concentrations of quercetin (Q), imipenem (IPM) and ciprofloxacin (CIP) alone and in combination against P. aeruginosa. Methods We determined the MIC and synergistic activity of Q, IPM and CIP against P. aeruginosa PAO1. The effect of sub-MIC (1/4 and 1/16 MIC) concentrations of Q, IPM and CIP alone and in combination on QS related virulence factors and expression of lasI and lasR genes was also determined. Results The addition of Q decreased the MIC of IPM and CIP and there was additive effect from these combinations. Quercetin did not show significant effects on cell growth at concentrations of 1/4 and 1/16 MIC. However, sub-MIC concentrations (1/4 and 1/16 MIC) of Q in combination with IPM or CIP showed the significant reduction in QS dependent phenotypes (P < 0.001). The combination of Q with IPM or CIP at the concentrations of 1/4 MIC showed the greatest reduction in the expression levels of lasI compared with untreated control (P < 0.001). The docking results suggested that quercetin has potential to inhibit the LasR protein. Conclusion Considering the beneficial biological effects of quercetin and its inhibitory effects on QS related virulence traits, the combination of this natural compound with imipenem or ciprofloxacin may be used as an effective approach for managing of multidrug resistant infections of P. aeruginosa.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Anti-quorum sensing activity of quercetin in combination with imipenem and ciprofloxacin against Pseudomonas aeruginosa PAO1

Taghavi

Zeighami

Heidari

et al. 2021

Preprint

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“…Using biofilm formation assay, Ouyang and colleagues [136] reported that quercetin decreases adhesion and biofilm formation in Pseudomonas aeruginosa, as well as swarming motility and expression of biofilm-associated genes. Quercetin also showed significant reduction in QS-dependent phenotypes, including violacein Quercetin has been investigated by several researchers for its anti-QS activities [79,83,[131][132][133][134][135][136]. Results revealed that the actions of quercetin against QS are diverse and multiple, and depend on the bacterial strain tested and the experimental method used.…”

Section: Pseudomonas Aeruginosamentioning

confidence: 99%

Mechanisms, Anti-Quorum-Sensing Actions, and Clinical Trials of Medicinal Plant Bioactive Compounds against Bacteria: A Comprehensive Review

et al. 2022

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Bacterial strains have developed an ability to resist antibiotics via numerous mechanisms. Recently, researchers conducted several studies to identify natural bioactive compounds, particularly secondary metabolites of medicinal plants, such as terpenoids, flavonoids, and phenolic acids, as antibacterial agents. These molecules exert several mechanisms of action at different structural, cellular, and molecular levels, which could make them candidates or lead compounds for developing natural antibiotics. Research findings revealed that these bioactive compounds can inhibit the synthesis of DNA and proteins, block oxidative respiration, increase membrane permeability, and decrease membrane integrity. Furthermore, recent investigations showed that some bacterial strains resist these different mechanisms of antibacterial agents. Researchers demonstrated that this resistance to antibiotics is linked to a microbial cell-to-cell communication system called quorum sensing (QS). Consequently, inhibition of QS or quorum quenching is a promising strategy to not only overcome the resistance problems but also to treat infections. In this respect, various bioactive molecules, including terpenoids, flavonoids, and phenolic acids, exhibit numerous anti-QS mechanisms via the inhibition of auto-inducer releases, sequestration of QS-mediated molecules, and deregulation of QS gene expression. However, clinical applications of these molecules have not been fully covered, which limits their use against infectious diseases. Accordingly, the aim of the present work was to discuss the role of the QS system in bacteria and its involvement in virulence and resistance to antibiotics. In addition, the present review summarizes the most recent and relevant literature pertaining to the anti-quorum sensing of secondary metabolites and its relationship to antibacterial activity.

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“…The stem bark of S. acoca contains quercetin, quercetin-3-O-α-lrhamnoside, kaempferol, betasitosterol [31]. Studies have shown that quercetin and its derivates posses significant anti-QS properties against C. violaceum and P. aeruginosa [32][33]. Possibly, the anti-QS activity of SAE may be attributed to its bioactive flavonoid contents.…”

Section: (Iii and Iv)mentioning

confidence: 99%

Evaluation of Anti-quorum Sensing Potential of Saraca asoca (Family Caesalpiniaceae) against Chromobacterium violaceum and Pseudomonas aeruginosa PA01

Paliya

Mathew

Singh

2021

JPRI

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Aim: The present study was performed to evaluate the anti-quorum sensing (QS) potential of traditional medicinal herb Saracaasoca (family Caesalpiniaceae) stem bark extract against Chromobacterium violaceum and Pseudomonas aeruginosa PA01. Study Design: First, the test sample (bark extract) was screened for anti-QS activity. Then systematic in-vitro and biochemical tests were performed to evaluate the effect of the test sample on the QS mediated virulence factors. Place and Duration of Study: All the experimental works were performed in Lab 311, pharmacology division, CSIR-NBRI Lucknow from June 2019 to October 2019. Methodology: The samples of Saraca asoca stem bark were washed, dried and extracted using 70% methanol. The minimum inhibitory concentration (MIC) of the prepared Sarca asoca bark extract was determined using the Alamar blue assay, and the anti-QS activity was screened using standard agar overlay method against CV 12472 at subinhibitory concentrations 100, 200 and 300 µg (< MIC value). SAE effect on biofilms formation was assessed by growing biofilms on glass slides in a static culture of PA01. Anti-virulence effect of SAE on the production of QS-regulated virulence factors such as Pyocyanin, proteases, elastases, rhamnolipid and alginate in Pseudomonas aeruginosa was determined using the supernatant of a 24 hours old broth culture of PA01 supplemented with SAE. Using the agar plate technique, the swimming and swarming motility assays were conducted on 0.3% and 0.5% agar plates respectively. One-way ANOVA was used to analyze the data, presented as mean ± SD (standard deviation) of three independent experiments. Results: Preliminary screening results showed significant QS inhibition against CV 12472 in an agar overlay disk diffusion assay in a concentration-dependent manner. Data from the biofilm assay showed loose, distorted, irregular PA01 biofilm formation at 200 µg (48%) and 300 µg (65%). SAE caused a significant drop in virulence factor production, with maximum reduction in pyocyanin (58%), proteases (67%), elastases (52%), rhamnolipid (53%), and alginate (44%) observed at 300 µg concentration. At SAE sub-lethal concentrations (200 and 300 µg), both the swimming and swarming motility of PA01 were significantly inhibited. Conclusions: The present study demonstrates the broad-spectrum anti-QS potential of SAE, reported for the first time, suggesting that SAE could be considered as an alternative herbal source to develop antimicrobial agents which can be either solitary or synergistically with conventional antimicrobial drugs.

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Anti-Quorum Sensing Potential of Antioxidant Quercetin and Resveratrol

Cited by 17 publications

References 26 publications

Anti-quorum sensing activity of quercetin in combination with imipenem and ciprofloxacin against Pseudomonas aeruginosa PAO1

Anti-quorum sensing activity of quercetin in combination with imipenem and ciprofloxacin against Pseudomonas aeruginosa PAO1

Mechanisms, Anti-Quorum-Sensing Actions, and Clinical Trials of Medicinal Plant Bioactive Compounds against Bacteria: A Comprehensive Review

Evaluation of Anti-quorum Sensing Potential of Saraca asoca (Family Caesalpiniaceae) against Chromobacterium violaceum and Pseudomonas aeruginosa PA01

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