Anti-staphylococcal activity, cytotoxicity, and chemical composition of hexane extracts from arils and seeds of two Samoan Myristica spp

Houdková, Markéta; Urbanová, Klára; Doskočil, Ivo; Soon, Julian Wong; Foliga, Talie; Nový, Pavel; Kokoška, Ladislav

doi:10.1016/j.sajb.2021.01.009

Cited by 4 publications

(2 citation statements)

References 21 publications

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“…As a positive control, doxorubicin was used. The levels of cytotoxic effects were categorized as cytotoxic (IC 50 2.00 µg/mL), moderately cytotoxic (IC 50 2.00–89.00 µg/mL), and non-toxic (IC 50 > 90.00 µg/mL) according to the Special Programme for Research and Training in Tropical Diseases (WHO—Tropical Diseases) [ 53 ]. The rate of the cytotoxicity (CT%) was estimated by the following expression: CT% = Ac − At/Ac × 100 In which, Ac and At are the absorbance of the control sample and the test sample, respectively.…”

Section: Methodsmentioning

confidence: 99%

Multifunctional Silver Nanoparticles Based on Chitosan: Antibacterial, Antibiofilm, Antifungal, Antioxidant, and Wound-Healing Activities

Shehabeldine

Salem

Ali

et al. 2022

JoF

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The purpose of this study is to create chitosan-stabilized silver nanoparticles (Chi/Ag-NPs) and determine whether they were cytotoxic and also to determine their characteristic antibacterial, antibiofilm, and wound healing activities. Recently, the development of an efficient and environmentally friendly method for synthesizing metal nanoparticles based on polysaccharides has attracted a lot of interest in the field of nanotechnology. Colloidal Chi/Ag-NPs are prepared by chemical reduction of silver ions in the presence of Chi, giving Chi/Ag-NPs. Physiochemical properties are determined by Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), transmission electron microscopy (TEM), dynamic light scattering (DLS), and scanning electron microscopy with energy-dispersive X-ray spectroscopy (SEM-EDX) analyses. TEM pictures indicate that the generated Chi/Ag-NPs are nearly spherical in shape with a thin chitosan covering around the Ag core and had sizes in the range of 9–65 nm. In vitro antibacterial activity was evaluated against Staphylococcus aureus and Pseudomonas aeruginosa by a resazurin-mediated microtiter plate assay. The highest activity was observed with the lowest concentration of Chi/Ag-NPs, which was 12.5 µg/mL for both bacterial strains. Additionally, Chi/Ag-NPs showed promising antifungal features against Candida albicans, Aspergillus fumigatus, Aspergillus terreus, and Aspergillus niger, where inhibition zones were 22, 29, 20, and 17 mm, respectively. Likewise, Chi/Ag-NPs revealed potential antioxidant activity is 92, 90, and 75% at concentrations of 4000, 2000, and 1000 µg/mL, where the IC50 of Chi/Ag-NPs was 261 µg/mL. Wound healing results illustrated that fibroblasts advanced toward the opening to close the scratch wound by roughly 50.5% after a 24-h exposure to Chi/Ag-NPs, greatly accelerating the wound healing process. In conclusion, a nanocomposite based on AgNPs and chitosan was successfully prepared and exhibited antibacterial, antibiofilm, antifungal, antioxidant, and wound healing activities that can be used in the medical field.

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Section: Methodsmentioning

confidence: 99%

Multifunctional Silver Nanoparticles Based on Chitosan: Antibacterial, Antibiofilm, Antifungal, Antioxidant, and Wound-Healing Activities

Shehabeldine

Salem

Ali

et al. 2022

JoF

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“…For MIC, different concentrations of the sample were prepared using prepared solvent, and the minimum concentration zone of inhibition was recorded through plate assay followed by broth microdilution method through the guidelines of the CLSI (Clinical and Laboratory Standards Institute). Every extract sample was dissolved in the prepared solvent and diluted in nutrient broth in a range of (0.015-1) mg/ml [25,26]. We performed the antimicrobial activity against standard streptomycin (active pharmaceutical ingredients) for better results.…”

Section: In Vitro Antimicrobial Studymentioning

confidence: 99%

Qualitative assessment of the flowering buds of Mesua ferrea Linn with special emphasize on HPTLC and universal DNA bar-coding technique and evaluation of its antimicrobial potential

Sutradhar,

Sarkar

2024

Futur J Pharm Sci

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Background The study has been scientifically exploring the powder sample and extracts of flowering buds of Mesua ferrea Linn (FBMF) based on pharmacognostic and phytochemical parameters. The medicinal plant Mesua ferrea Linn was identified by Botanical verification and the universal DNA bar-coding technique. The FBMF powder’s quality was performed by the micromeritics properties like bulk density, tapped density, angle of repose, Hausner ratio, Carr’s index, and optical microscopy method, and physicochemical evaluations were performed by the swelling index, foaming index, loss on drying, extractive values, and ash values. The extract’s preliminary phytochemical screening was accomplished by the alkaloids, flavonoids, tannins, steroids, carbohydrates, and glycoside tests. To ensure the presence of a bioactive compound of each FBMF extract by qualitative HPTLC study against the reference β-sitosterol Rf value of 0.83 was revealed at 254 nm with a developed solvent system toluene, ethyl acetate, and acetic acid (6:2:0.1) v/v. Many pharmacological benefits, including those related to wound healing, nonalcoholic fatty liver disease, analgesic, sedative effects, immunomodulatory, anticancer, antimicrobial, anti-inflammatory, anxiolytic, and atopic dermatitis, have been demonstrated by this phytosterol. An antimicrobial study was carried out by a well diffusion method and, lastly, measured minimum inhibitory concentration and compared to the marketed active pharmaceutical component. Numerous skin infections of more invasive, serious illnesses are caused by potential antimicrobial activity. Results The universal DNA bar-coding of plant Mesua ferrea Linn has shown a high percentage of identity. The micrometrics properties and physicochemical evaluation of the powder sample of FBMF were in an acceptable range. The preliminary phytochemical screening shows that all extracts contain steroids and carbohydrates. Moreover, flavonoids were found in ethyl acetate and ethanol, and a qualitative HPTLC study confirmed that every extract contains β-sitosterol. N-hexane extract of FBMF shows the potential antimicrobial activity with Staphylococcus aureus, and the MIC value was observed at 0.062 mg/ml. Conclusion Our research demonstrated that the plant Mesua ferrea Linn has been successfully authenticated by a DNA bar-coding technique, and all extracts of FBMF contain β-sitosterol. The n-hexane solvent extracts have shown the potential highest antimicrobial effects compared to other extracts. These results support using n-hexane extracts as a traditional medicine for treating several diseases. Graphical Abstract

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Malabaricones from the fruit of Myristica cinnamomea King as potential agents against Acanthamoeba castellanii

Ahmed,

Sivasothy,

Khan

et al. 2023

Acta Tropica

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Anti-staphylococcal activity, cytotoxicity, and chemical composition of hexane extracts from arils and seeds of two Samoan Myristica spp

Cited by 4 publications

References 21 publications

Multifunctional Silver Nanoparticles Based on Chitosan: Antibacterial, Antibiofilm, Antifungal, Antioxidant, and Wound-Healing Activities

Multifunctional Silver Nanoparticles Based on Chitosan: Antibacterial, Antibiofilm, Antifungal, Antioxidant, and Wound-Healing Activities

Qualitative assessment of the flowering buds of Mesua ferrea Linn with special emphasize on HPTLC and universal DNA bar-coding technique and evaluation of its antimicrobial potential

Malabaricones from the fruit of Myristica cinnamomea King as potential agents against Acanthamoeba castellanii

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