Anti-Thrombosis Activity of Sinapic Acid Isolated from the Lees of Bokbunja Wine

Abstract

From the lees of bokbunja wine (LBW) made from Rubus coreanus Miquel, we have identified six compounds (1: trans-4-hydroxycinnamic acid; 2: trans-4-hydroxy-3-methoxycinnamic acid; 3: 3,4-dihydroxycinnamic acid; 4: 4-hydroxy-3-methoxybenzoic acid; 5: 3,5-dimethoxy-4- hydroxybenzoic acid; and 6: 3,5-dimethoxy-4-hydroxycinnamic acid (sinapic acid)) through silica gel chromatography and UHPLC-MS. The compounds 1-6 showed strong anticoagulation and platelet aggregation inhibitory activities without hemolytic effect against human red blood cells. To date, this is the first report of the in vitro anti-thrombosis activity of sinapic acid. Our results suggest that different cinnamic and benzoic acid derivatives are closely linked to the anti-thrombosis activity of LBW, and sinapic acid could be developed as a promising anti-thrombosis agent.

Phenolic fractions from nine Trifolium species modulate the coagulant properties of blood plasma in vitro without cytotoxicity towards blood cells

“…Other work of those authors revealed the allosteric inhibition of thrombin ( via the exosite II) by chemo‐enzymatic, sulfated dehydropolymers of 4‐hydroxycinnamic acids. Antithrombotic effects of sinapic acid (3,5‐dimethoxy‐4‐hydroxycinnamic acid) have been recently described by Kim et al . It should be noted, however, that the authors investigated the anticoagulant effect of this compound at significantly higher concentrations (1.3–5 mg/ml), when compared to the concentration range of Trifolium extracts, used in our studies.…”

Spatial and Age Related Effects on Phytochemical Compounds of Ethanolic Extract of <em>Gigantochloa scortechinii </em>Rhizome

“…Rubus coreanus and showed strong anti-coagulation and platelet aggregation inhibitory activities without hemolytic effect against human blood cells [59]. Syringic acid isolated from truffle were present in all three study sites and all four rhizome ages except for new sprout at Kenaboi FR (Table 2, and 4).…”

Antithrombotic Potential of Red Allium cepa and Angelica gigas Nakai

“…The samples were initially dissolved in dimethyl sulfoxide (DMSO) and the anti-coagulation activity was then assessed by measuring the clotting times required for activation of thrombin (TT: thrombin time), prothrombin (PT: Prothrombin time) and coagulation factor (aPTT: active partial thromboplastin time). 26 TT, PT and aPTT assays were conducted according to the manufacturer's protocols (Ameulungcoagulameter, Lemgo, Germany). Aspirin (1.5 mg/mL, acetylsalicylic acid, Sigma, St. Louis, MO, USA) was used as a positive control, as it played a role inhibiting thrombin generation and clot formation at high doses (300-500 mg/kg).…”

“…Area under the ROC (receiver operating characteristics) curve (AUC) was calculated according to the platelet aggregation curve. 26 Platelet aggregation was calculated according to the following equation: (AUC of samples/AUC of DMSO) × 100. 28 Aspirin was used as a positive control for anti-platelet aggregation, as it inhibits COX-1 and reduces thromboxane A2 at low concentrations (100 mg/day).…”